ID   YNAI_ECO57              Reviewed;         343 AA.
AC   P0AEB6; P77253;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Low conductance mechanosensitive channel YnaI;
GN   Name=ynaI; OrderedLocusNames=Z2437, ECs1912;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- SUBUNIT: Homoheptamer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG56469.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB35335.1; -; Genomic_DNA.
DR   PIR; A85751; A85751.
DR   PIR; H90867; H90867.
DR   RefSeq; NP_309939.1; NC_002695.1.
DR   RefSeq; WP_000559900.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AEB6; -.
DR   SMR; P0AEB6; -.
DR   STRING; 155864.EDL933_2345; -.
DR   EnsemblBacteria; AAG56469; AAG56469; Z2437.
DR   EnsemblBacteria; BAB35335; BAB35335; ECs_1912.
DR   GeneID; 66674841; -.
DR   GeneID; 912403; -.
DR   KEGG; ece:Z2437; -.
DR   KEGG; ecs:ECs_1912; -.
DR   PATRIC; fig|386585.9.peg.2018; -.
DR   eggNOG; COG0668; Bacteria.
DR   HOGENOM; CLU_037945_0_0_6; -.
DR   OMA; ITQRVEN; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   Gene3D; 2.30.30.60; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR006685; MscS_channel.
DR   InterPro; IPR011066; MscS_channel_C.
DR   InterPro; IPR006686; MscS_channel_CS.
DR   InterPro; IPR011014; MscS_channel_TM-2.
DR   InterPro; IPR023408; MscS_dom_sf.
DR   InterPro; IPR045042; YnaI-like.
DR   PANTHER; PTHR43634; PTHR43634; 1.
DR   Pfam; PF00924; MS_channel; 1.
DR   SUPFAM; SSF50182; SSF50182; 1.
DR   SUPFAM; SSF82689; SSF82689; 1.
DR   SUPFAM; SSF82861; SSF82861; 1.
DR   PROSITE; PS01246; UPF0003; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion channel; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..343
FT                   /note="Low conductance mechanosensitive channel YnaI"
FT                   /id="PRO_0000110243"
FT   TOPO_DOM        1..9
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        31..40
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        62..77
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        99..125
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        147
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..343
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   343 AA;  38755 MW;  469F0B7909480D13 CRC64;
     MIAELFTNNA LNLVIIFGSC AALILMSFWF RRGNRKRKGF LFHAVQFLIY TIIISAVGSI
     INYVIENYKL KFITPGVIDF ICTSLIAVIL TIKLFLLINQ FEKQQIKKGR DITSARIMSR
     IIKITIIVVL VLLYGEHFGM SLSGLLTFGG IGGLAVGMAG KDILSNFFSG IMLYFDRPFS
     IGDWIRSPDR NIEGTVAEIG WRITKITTFD NRPLYVPNSL FSSISVENPG RMTNRRITTT
     IGLRYEDAAK VGVIVEAVRE MLKNHPAIDQ RQTLLVYFNQ FADSSLNIMV YCFTKTTVWA
     EWLAAQQDVY LKIIDIVQSH GADFAFPSQT LYMDNITPPE QGR