ID   YNFA_ECOLI              Reviewed;         108 AA.
AC   P76169; Q2MB80;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=UPF0060 membrane protein YnfA {ECO:0000255|HAMAP-Rule:MF_00010};
GN   Name=ynfA {ECO:0000255|HAMAP-Rule:MF_00010};
GN   OrderedLocusNames=b1582, JW1574;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   SUBCELLULAR LOCATION, AND TOPOLOGY.
RC   STRAIN=K12 / JM109 / ATCC 53323;
RX   PubMed=11867724; DOI=10.1073/pnas.052018199;
RA   Drew D., Sjoestrand D., Nilsson J., Urbig T., Chin C.-N., de Gier J.-W.,
RA   von Heijne G.;
RT   "Rapid topology mapping of Escherichia coli inner-membrane proteins by
RT   prediction and PhoA/GFP fusion analysis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:2690-2695(2002).
RN   [4]
RP   SUBCELLULAR LOCATION, AND TOPOLOGY.
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00010, ECO:0000269|PubMed:11867724,
CC       ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_00010, ECO:0000269|PubMed:11867724,
CC       ECO:0000269|PubMed:15919996}.
CC   -!- SIMILARITY: Belongs to the UPF0060 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00010}.
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DR   EMBL; U00096; AAC74654.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76476.1; -; Genomic_DNA.
DR   PIR; H64913; H64913.
DR   RefSeq; NP_416099.1; NC_000913.3.
DR   RefSeq; WP_000598292.1; NZ_STEB01000003.1.
DR   AlphaFoldDB; P76169; -.
DR   BioGRID; 4260820; 20.
DR   STRING; 511145.b1582; -.
DR   TCDB; 2.A.7.26.1; the drug/metabolite transporter (dmt) superfamily.
DR   PaxDb; P76169; -.
DR   PRIDE; P76169; -.
DR   DNASU; 946125; -.
DR   EnsemblBacteria; AAC74654; AAC74654; b1582.
DR   EnsemblBacteria; BAE76476; BAE76476; BAE76476.
DR   GeneID; 66674528; -.
DR   GeneID; 946125; -.
DR   KEGG; ecj:JW1574; -.
DR   KEGG; eco:b1582; -.
DR   PATRIC; fig|1411691.4.peg.680; -.
DR   EchoBASE; EB3600; -.
DR   eggNOG; COG1742; Bacteria.
DR   HOGENOM; CLU_117653_2_1_6; -.
DR   InParanoid; P76169; -.
DR   OMA; WLWAVEG; -.
DR   PhylomeDB; P76169; -.
DR   BioCyc; EcoCyc:G6840-MON; -.
DR   PRO; PR:P76169; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoliWiki.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   HAMAP; MF_00010; UPF0060; 1.
DR   InterPro; IPR003844; UPF0060.
DR   PANTHER; PTHR36116; PTHR36116; 1.
DR   Pfam; PF02694; UPF0060; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..108
FT                   /note="UPF0060 membrane protein YnfA"
FT                   /id="PRO_0000162329"
FT   TOPO_DOM        1..5
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00010"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00010"
FT   TOPO_DOM        27..30
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00010"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00010"
FT   TOPO_DOM        52..60
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00010"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00010"
FT   TOPO_DOM        82..84
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00010"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00010"
FT   TOPO_DOM        106..108
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00010"
SQ   SEQUENCE   108 AA;  11920 MW;  16319B7E35F2D1AF CRC64;
     MIKTTLLFFA TALCEIIGCF LPWLWLKRNA SIWLLLPAGI SLALFVWLLT LHPAASGRVY
     AAYGGVYVCT ALMWLRVVDG VKLTLYDWTG ALIALCGMLI IVAGWGRT