ID   YNHF_ECOLI              Reviewed;          29 AA.
AC   A5A618;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Uncharacterized protein YnhF;
GN   Name=ynhF; OrderedLocusNames=b4602, JW1649.1;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   SUBCELLULAR LOCATION, AND INDUCTION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=19121005; DOI=10.1111/j.1365-2958.2008.06495.x;
RA   Hemm M.R., Paul B.J., Schneider T.D., Storz G., Rudd K.E.;
RT   "Small membrane proteins found by comparative genomics and ribosome binding
RT   site models.";
RL   Mol. Microbiol. 70:1487-1501(2008).
RN   [4]
RP   INDUCTION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=19734316; DOI=10.1128/jb.00872-09;
RA   Hemm M.R., Paul B.J., Miranda-Rios J., Zhang A., Soltanzad N., Storz G.;
RT   "Small stress response proteins in Escherichia coli: proteins missed by
RT   classical proteomic studies.";
RL   J. Bacteriol. 192:46-58(2010).
RN   [5]
RP   IDENTIFICATION, MASS SPECTROMETRY, AND FORMYLATION AT MET-1.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=21050835; DOI=10.1016/j.ab.2010.10.035;
RA   Guan Z., Wang X., Raetz C.R.;
RT   "Identification of a chloroform-soluble membrane miniprotein in Escherichia
RT   coli and its homolog in Salmonella typhimurium.";
RL   Anal. Biochem. 409:284-289(2011).
RN   [6]
RP   SUBCELLULAR LOCATION, AND TOPOLOGY.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=21778229; DOI=10.1074/jbc.m111.245696;
RA   Fontaine F., Fuchs R.T., Storz G.;
RT   "Membrane localization of small proteins in Escherichia coli.";
RL   J. Biol. Chem. 286:32464-32474(2011).
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305|PubMed:19121005,
CC       ECO:0000305|PubMed:21778229}; Single-pass membrane protein
CC       {ECO:0000269|PubMed:19121005, ECO:0000269|PubMed:21778229}. Note=May be
CC       able to insert into the membrane in both orientations.
CC   -!- INDUCTION: Constitutively expressed (PubMed:19121005), repressed on
CC       shift from glucose minimal to glycerol minimal medium, induced in low
CC       oxygen (PubMed:19121005) (at protein level).
CC       {ECO:0000269|PubMed:19121005, ECO:0000269|PubMed:19734316}.
CC   -!- MASS SPECTROMETRY: Mass=3023.740; Method=Electrospray; Note=Includes N-
CC       formyl-Met.; Evidence={ECO:0000269|PubMed:21050835};
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DR   EMBL; U00096; ABP93447.1; -; Genomic_DNA.
DR   EMBL; AP009048; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; WP_000102278.1; NZ_STEB01000003.1.
DR   RefSeq; YP_001165321.1; NC_000913.3.
DR   PDB; 6RKO; EM; 2.68 A; H=1-29.
DR   PDBsum; 6RKO; -.
DR   AlphaFoldDB; A5A618; -.
DR   SMR; A5A618; -.
DR   STRING; 511145.b4602; -.
DR   TCDB; 3.D.4.3.2; the proton-translocating cytochrome oxidase (cox) superfamily.
DR   jPOST; A5A618; -.
DR   PaxDb; A5A618; -.
DR   EnsemblBacteria; ABP93447; ABP93447; b4602.
DR   GeneID; 5061505; -.
DR   GeneID; 67415638; -.
DR   KEGG; eco:b4602; -.
DR   PATRIC; fig|511145.12.peg.1730; -.
DR   BioCyc; EcoCyc:MON0-2822; -.
DR   BioCyc; MetaCyc:MON0-2822; -.
DR   PRO; PR:A5A618; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0070069; C:cytochrome complex; IDA:EcoCyc.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IDA:EcoCyc.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0071456; P:cellular response to hypoxia; IEP:EcoCyc.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Formylation; Membrane;
KW   Reference proteome; Stress response; Transmembrane; Transmembrane helix.
FT   CHAIN           1..29
FT                   /note="Uncharacterized protein YnhF"
FT                   /id="PRO_0000311789"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         1
FT                   /note="N-formylmethionine"
FT                   /evidence="ECO:0000269|PubMed:21050835"
FT   HELIX           4..27
FT                   /evidence="ECO:0007829|PDB:6RKO"
SQ   SEQUENCE   29 AA;  2998 MW;  B09FFA108DF6BF5B CRC64;
     MSTDLKFSLV TTIIVLGLIV AVGLTAALH