ID   YNL5_YEAS7              Reviewed;         644 AA.
AC   A6ZRW8; B0KZS0;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Uncharacterized vacuolar membrane protein SCY_4679;
GN   ORFNames=SCY_4679;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 352-644.
RX   PubMed=18780730; DOI=10.1534/genetics.108.092932;
RA   Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S.,
RA   Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H.,
RA   Steinmetz L.M.;
RT   "Sequential elimination of major-effect contributors identifies additional
RT   quantitative trait loci conditioning high-temperature growth in yeast.";
RL   Genetics 180:1661-1670(2008).
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFW02000067; EDN62700.1; -; Genomic_DNA.
DR   EMBL; EF125227; ABN58641.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZRW8; -.
DR   SMR; A6ZRW8; -.
DR   ESTHER; yeast-ynl5; 6_AlphaBeta_hydrolase.
DR   PRIDE; A6ZRW8; -.
DR   EnsemblFungi; EDN62700; EDN62700; SCY_4679.
DR   HOGENOM; CLU_028296_0_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR019431; DUF2417.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   Pfam; PF10329; DUF2417; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Membrane; Phosphoprotein; Transmembrane; Transmembrane helix; Vacuole.
FT   CHAIN           1..644
FT                   /note="Uncharacterized vacuolar membrane protein SCY_4679"
FT                   /id="PRO_0000378317"
FT   TOPO_DOM        1..90
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        112..122
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        144..147
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..174
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        196..271
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        272..292
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        293..644
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000250"
FT   DOMAIN          348..619
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   REGION          1..39
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          225..251
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          469..492
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        7..24
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        470..492
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         22
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P53925"
FT   MOD_RES         56
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P53925"
FT   MOD_RES         63
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P53925"
FT   MOD_RES         244
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P53925"
SQ   SEQUENCE   644 AA;  74138 MW;  F45BF40CEC666838 CRC64;
     MKANGLDNDP ARTRMERTDI DSEHPEAQPL LNNNHRTLGA GSANGPAVNE GRDIESDGFI
     KDSLFQIRKG YRIFIHNSKW ILNILILINT IWLVTTLISD FFFNINILFG FSNRYASFND
     LTLIFISIIA NSFNLWFNKL GLYSALDYSL NVTLCVLTLF NLALTYLIKY TRQRIGFVGT
     FTYLWTSFSF FIGAILDWYL LFYNNSINEP LEERRIDDAN ISTFNENHTN STENRDRSQY
     GSGSPTPTHR SQLVQNKHTL TEWVSIGFRN TIKFLILIFF ALFTLNTLLT TLDTYRLTHK
     LPITVQSPSY EAFHYVDAAK TYQLHITCYG DVFDQENNTD LSENKKQPII LFEHGGYDTG
     YLSATWIEEL YHLDKIQRYC LYDRPGYGLS DSPPAPISIA MVAESLRYAL IKDAKIKGPF
     TTVGYDLGGL FTRVFTAKNV DIVDSMMLVE SWHEELLLKN YIQRLLPPGR GDGDDGDDGN
     GNDGDGRNHD KTWLPSEIER HNEFRLWWKG IWSSLGWRLQ TSWLLAHHGS KERIYGRDMK
     YQGRFLRSKF LESVTSSILS YRDVTNNAES LQNVKTSIVS SKEMVKKSAL WGDWQRDLTK
     ISHKTQEWKI VEGGHEIYKY GLGKQQTQEV LLRLIGELGK LTED