YNM2_YEAST
ID YNM2_YEAST Reviewed; 115 AA.
AC P53921; B0KZR4; B0KZU1; B0KZY6; D6W161;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=54S ribosomal protein bL35m {ECO:0000303|PubMed:24675956};
DE AltName: Full=Mitochondrial large ribosomal subunit protein bL35m {ECO:0000303|PubMed:24675956};
DE Flags: Precursor;
GN OrderedLocusNames=YNL122C; ORFNames=N1901;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS GLN-11 AND PRO-31.
RC STRAIN=ATCC 200060 / W303, S103, SK1, V1-09, YJM 1129, YJM 269, YJM 270,
RC YJM 320, YJM 326, YJM 339, YJM 627, and YJM230;
RX PubMed=18780730; DOI=10.1534/genetics.108.092932;
RA Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S.,
RA Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H.,
RA Steinmetz L.M.;
RT "Sequential elimination of major-effect contributors identifies additional
RT quantitative trait loci conditioning high-temperature growth in yeast.";
RL Genetics 180:1661-1670(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9090055;
RX DOI=10.1002/(sici)1097-0061(19970315)13:3<261::aid-yea64>3.0.co;2-l;
RA de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D.,
RA Pallavicini A., Lanfranchi G., Valle G.;
RT "The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces
RT cerevisiae reveals an unusually high number of overlapping open reading
RT frames.";
RL Yeast 13:261-266(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=17054397; DOI=10.1371/journal.pgen.0020170;
RA Perocchi F., Jensen L.J., Gagneur J., Ahting U., von Mering C., Bork P.,
RA Prokisch H., Steinmetz L.M.;
RT "Assessing systems properties of yeast mitochondria through an interaction
RT map of the organelle.";
RL PLoS Genet. 2:E170-E170(2006).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=25609543; DOI=10.1038/ncomms7019;
RA Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.;
RT "Organization of the mitochondrial translation machinery studied in situ by
RT cryoelectron tomography.";
RL Nat. Commun. 6:6019-6019(2015).
RN [9]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.20 ANGSTROMS), AND SUBUNIT.
RX PubMed=24675956; DOI=10.1126/science.1249410;
RA Amunts A., Brown A., Bai X.C., Llacer J.L., Hussain T., Emsley P., Long F.,
RA Murshudov G., Scheres S.H., Ramakrishnan V.;
RT "Structure of the yeast mitochondrial large ribosomal subunit.";
RL Science 343:1485-1489(2014).
CC -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC mitochondrial genome-encoded proteins, including at least some of the
CC essential transmembrane subunits of the mitochondrial respiratory
CC chain. The mitoribosomes are attached to the mitochondrial inner
CC membrane and translation products are cotranslationally integrated into
CC the membrane. {ECO:0000305|PubMed:24675956,
CC ECO:0000305|PubMed:25609543}.
CC -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit (mt-
CC LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S)
CC and a large (54S) subunit. The 37S small subunit contains a 15S
CC ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large
CC subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.
CC {ECO:0000269|PubMed:24675956}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:17054397}. Note=Mitoribosomes are tethered to the
CC mitochondrial inner membrane and spatially aligned with the membrane
CC insertion machinery through two distinct membrane contact sites, formed
CC by the 21S rRNA expansion segment 96-ES1 and the inner membrane protein
CC MBA1. {ECO:0000269|PubMed:25609543}.
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL35 family.
CC {ECO:0000305}.
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DR EMBL; EF125216; ABN58536.1; -; Genomic_DNA.
DR EMBL; EF125217; ABN58545.1; -; Genomic_DNA.
DR EMBL; EF125218; ABN58554.1; -; Genomic_DNA.
DR EMBL; EF125219; ABN58563.1; -; Genomic_DNA.
DR EMBL; EF125220; ABN58572.1; -; Genomic_DNA.
DR EMBL; EF125221; ABN58581.1; -; Genomic_DNA.
DR EMBL; EF125222; ABN58590.1; -; Genomic_DNA.
DR EMBL; EF125223; ABN58599.1; -; Genomic_DNA.
DR EMBL; EF125224; ABN58608.1; -; Genomic_DNA.
DR EMBL; EF125225; ABN58617.1; -; Genomic_DNA.
DR EMBL; EF125226; ABN58626.1; -; Genomic_DNA.
DR EMBL; EF125228; ABN58644.1; -; Genomic_DNA.
DR EMBL; Z69382; CAA93385.1; -; Genomic_DNA.
DR EMBL; Z71398; CAA96003.1; -; Genomic_DNA.
DR EMBL; AY692589; AAT92608.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10427.1; -; Genomic_DNA.
DR PIR; S63063; S63063.
DR RefSeq; NP_014277.1; NM_001182960.1.
DR PDB; 3J6B; EM; 3.20 A; Z=1-115.
DR PDB; 5MRC; EM; 3.25 A; Z=54-115.
DR PDB; 5MRE; EM; 3.75 A; Z=54-115.
DR PDB; 5MRF; EM; 4.97 A; Z=54-115.
DR PDBsum; 3J6B; -.
DR PDBsum; 5MRC; -.
DR PDBsum; 5MRE; -.
DR PDBsum; 5MRF; -.
DR AlphaFoldDB; P53921; -.
DR SMR; P53921; -.
DR BioGRID; 35705; 93.
DR ComplexPortal; CPX-1602; 54S mitochondrial large ribosomal subunit.
DR DIP; DIP-2009N; -.
DR IntAct; P53921; 3.
DR MINT; P53921; -.
DR STRING; 4932.YNL122C; -.
DR PaxDb; P53921; -.
DR PRIDE; P53921; -.
DR EnsemblFungi; YNL122C_mRNA; YNL122C; YNL122C.
DR GeneID; 855601; -.
DR KEGG; sce:YNL122C; -.
DR SGD; S000005066; YNL122C.
DR VEuPathDB; FungiDB:YNL122C; -.
DR eggNOG; ENOG502S7SR; Eukaryota.
DR HOGENOM; CLU_166987_1_0_1; -.
DR InParanoid; P53921; -.
DR BioCyc; YEAST:G3O-33143-MON; -.
DR PRO; PR:P53921; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53921; protein.
DR GO; GO:0015934; C:large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; IC:ComplexPortal.
DR GO; GO:0005762; C:mitochondrial large ribosomal subunit; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR GO; GO:0032543; P:mitochondrial translation; IC:SGD.
DR Gene3D; 4.10.410.60; -; 1.
DR InterPro; IPR021137; Ribosomal_L35.
DR InterPro; IPR037229; Ribosomal_L35_sf.
DR Pfam; PF01632; Ribosomal_L35p; 1.
DR SUPFAM; SSF143034; SSF143034; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Mitochondrion; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..115
FT /note="54S ribosomal protein bL35m"
FT /id="PRO_0000203430"
FT VARIANT 11
FT /note="R -> Q (in strain: V1-09 and YJM339)"
FT /evidence="ECO:0000269|PubMed:18780730"
FT VARIANT 31
FT /note="S -> P (in strain: YJM269, YJM270, YJM326 and
FT YJM1129)"
FT /evidence="ECO:0000269|PubMed:18780730"
SQ SEQUENCE 115 AA; 13244 MW; B6C9DC84CE1EB016 CRC64;
MKISLHNKRQ RGDQNQNMSV FNVLKPLLKG SNSFKVKLNG FLFNNVSTIT IRTLMKTHKG
TAKRWRRTGN TFKRGIAGRK HGNIGWSHRS LKALTGRKIA HPAYSKHLKR LLPYH
