YNS1_YEAST
ID YNS1_YEAST Reviewed; 407 AA.
AC P53878; D6W105;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Uncharacterized oxidoreductase YNL181W;
DE EC=1.-.-.-;
GN OrderedLocusNames=YNL181W; ORFNames=N1640;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 262.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Single-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 13900 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; Z71457; CAA96073.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10371.2; -; Genomic_DNA.
DR PIR; S63136; S63136.
DR RefSeq; NP_014218.2; NM_001183019.2.
DR AlphaFoldDB; P53878; -.
DR SMR; P53878; -.
DR BioGRID; 35651; 1043.
DR DIP; DIP-6413N; -.
DR IntAct; P53878; 2.
DR STRING; 4932.YNL181W; -.
DR iPTMnet; P53878; -.
DR MaxQB; P53878; -.
DR PaxDb; P53878; -.
DR PRIDE; P53878; -.
DR EnsemblFungi; YNL181W_mRNA; YNL181W; YNL181W.
DR GeneID; 855540; -.
DR KEGG; sce:YNL181W; -.
DR SGD; S000005125; YNL181W.
DR VEuPathDB; FungiDB:YNL181W; -.
DR eggNOG; KOG1208; Eukaryota.
DR HOGENOM; CLU_010194_44_1_1; -.
DR InParanoid; P53878; -.
DR OMA; NIEDPLW; -.
DR BioCyc; YEAST:G3O-33192-MON; -.
DR Reactome; R-SCE-5365859; RA biosynthesis pathway.
DR PRO; PR:P53878; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53878; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; HDA:SGD.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; ISS:SGD.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endoplasmic reticulum; Membrane; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..407
FT /note="Uncharacterized oxidoreductase YNL181W"
FT /id="PRO_0000203405"
FT TOPO_DOM 1..290
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..407
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 383..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 361..390
FT /evidence="ECO:0000255"
FT BINDING 57..79
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT CONFLICT 262
FT /note="A -> P (in Ref. 1; CAA96073)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 407 AA; 46503 MW; E57B677EB79BC2C9 CRC64;
MPLNIIGTAL LDGTDKIPYY QTIKKVAPYV LATGAIKYWS RGPSNTWERK LHGKVYLVTG
ATSQGMGTSV AYKMAELGAQ LIILTREVDE WVTEWCEELR EKTKNELIFV EKCDLSNLWE
IRKFATSWLD NSPPRRLDGV IVMSGDMEPW GIPKISLPQR RSSKDGLELQ IATNYVAIFH
LLNLLQPSFK AQPPDRDVRI ILATCWLQVV GDINIEDPLW QNAKYKSALK FFASSKLQLG
LSMMELQRRL TEDIKNQKTN GAERTGKNVT ITMVQPGTMR SNSLRRVISN GSVVLLIILY
CILLYPILWL FTKSGRRGDQ SFLYALMTPE LEEVNLKDTK VKYISDCSIV KFARKEFDDE
ELQKKLFDNT ERDILQLEKK VAAKRNANKT GNQNSKKKSQ NKSRKDD
