YOAJ_BACSU
ID YOAJ_BACSU Reviewed; 232 AA.
AC O34918; Q796F6;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Expansin-YoaJ;
DE AltName: Full=EXLX1;
DE Flags: Precursor;
GN Name=yoaJ; OrderedLocusNames=BSU18630;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequence analysis of the Bacillus subtilis chromosome region between the
RT terC and odhAB loci cloned in a yeast artificial chromosome.";
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 26-232, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=18971341; DOI=10.1073/pnas.0809382105;
RA Kerff F., Amoroso A., Herman R., Sauvage E., Petrella S., Filee P.,
RA Charlier P., Joris B., Tabuchi A., Nikolaidis N., Cosgrove D.J.;
RT "Crystal structure and activity of Bacillus subtilis YoaJ (EXLX1), a
RT bacterial expansin that promotes root colonization.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:16876-16881(2008).
CC -!- FUNCTION: May promote colonization of plant roots. May cause loosening
CC and extension of plant cell walls by disrupting non-covalent bonding
CC between cellulose microfibrils and matrix glucans. Has very low
CC expansin activity (in vitro). No enzymatic activity has been found.
CC Binds to peptidoglycan and to plant cell walls.
CC {ECO:0000269|PubMed:18971341}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000269|PubMed:18971341}.
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DR EMBL; AF027868; AAB84448.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13755.1; -; Genomic_DNA.
DR PIR; D69896; D69896.
DR RefSeq; NP_389744.1; NC_000964.3.
DR RefSeq; WP_003231419.1; NZ_JNCM01000036.1.
DR PDB; 2BH0; X-ray; 2.50 A; A=26-232.
DR PDB; 3D30; X-ray; 1.90 A; A=26-232.
DR PDB; 4FER; X-ray; 2.10 A; A/B=26-232.
DR PDB; 4FFT; X-ray; 2.10 A; A/B=26-232.
DR PDB; 4FG2; X-ray; 2.10 A; A/B=26-232.
DR PDB; 4FG4; X-ray; 2.70 A; A/B=26-232.
DR PDBsum; 2BH0; -.
DR PDBsum; 3D30; -.
DR PDBsum; 4FER; -.
DR PDBsum; 4FFT; -.
DR PDBsum; 4FG2; -.
DR PDBsum; 4FG4; -.
DR AlphaFoldDB; O34918; -.
DR SMR; O34918; -.
DR STRING; 224308.BSU18630; -.
DR CAZy; CBM63; Carbohydrate-Binding Module Family 63.
DR PaxDb; O34918; -.
DR PRIDE; O34918; -.
DR EnsemblBacteria; CAB13755; CAB13755; BSU_18630.
DR GeneID; 940108; -.
DR KEGG; bsu:BSU18630; -.
DR PATRIC; fig|224308.179.peg.2031; -.
DR eggNOG; COG4305; Bacteria.
DR InParanoid; O34918; -.
DR OMA; RNHKYPV; -.
DR BioCyc; BSUB:BSU18630-MON; -.
DR EvolutionaryTrace; O34918; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR Gene3D; 2.40.40.10; -; 1.
DR Gene3D; 2.60.40.760; -; 1.
DR InterPro; IPR036749; Expansin_CBD_sf.
DR InterPro; IPR009009; RlpA-like_DPBB.
DR InterPro; IPR036908; RlpA-like_sf.
DR Pfam; PF03330; DPBB_1; 1.
DR SUPFAM; SSF49590; SSF49590; 1.
DR SUPFAM; SSF50685; SSF50685; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..232
FT /note="Expansin-YoaJ"
FT /id="PRO_0000359936"
FT DOMAIN 58..127
FT /note="Expansin-like EG45"
FT STRAND 31..37
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 58..61
FT /evidence="ECO:0007829|PDB:3D30"
FT HELIX 63..66
FT /evidence="ECO:0007829|PDB:3D30"
FT HELIX 68..70
FT /evidence="ECO:0007829|PDB:3D30"
FT TURN 72..77
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 79..84
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 87..96
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 105..107
FT /evidence="ECO:0007829|PDB:3D30"
FT HELIX 109..115
FT /evidence="ECO:0007829|PDB:3D30"
FT HELIX 118..120
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 121..130
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 139..142
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 147..157
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 162..169
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 172..178
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 184..187
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 191..200
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 205..210
FT /evidence="ECO:0007829|PDB:3D30"
FT STRAND 222..225
FT /evidence="ECO:0007829|PDB:3D30"
SQ SEQUENCE 232 AA; 25638 MW; 4182A234D6224D46 CRC64;
MKKIMSAFVG MVLLTIFCFS PQASAAYDDL HEGYATYTGS GYSGGAFLLD PIPSDMEITA
INPADLNYGG VKAALAGSYL EVEGPKGKTT VYVTDLYPEG ARGALDLSPN AFRKIGNMKD
GKINIKWRVV KAPITGNFTY RIKEGSSRWW AAIQVRNHKY PVMKMEYEKD GKWINMEKMD
YNHFVSTNLG TGSLKVRMTD IRGKVVKDTI PKLPESGTSK AYTVPGHVQF PE
