YOBK_BACSU
ID YOBK_BACSU Reviewed; 152 AA.
AC O34596;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Immunity protein YobK;
GN Name=yobK; OrderedLocusNames=BSU18990;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequence analysis of the Bacillus subtilis chromosome region between the
RT terC and odhAB loci cloned in a yeast artificial chromosome.";
RL Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION AS AN IMMUNITY PROTEIN, INTERACTION WITH YOBL, AND EXPRESSION IN
RP E.COLI.
RC STRAIN=168;
RX PubMed=22200572; DOI=10.1016/j.febslet.2011.12.020;
RA Holberger L.E., Garza-Sanchez F., Lamoureux J., Low D.A., Hayes C.S.;
RT "A novel family of toxin/antitoxin proteins in Bacillus species.";
RL FEBS Lett. 586:132-136(2012).
RN [4]
RP FUNCTION AS AN IMMUNITY PROTEIN, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=34280190; DOI=10.1371/journal.pgen.1009682;
RA Kobayashi K.;
RT "Diverse LXG toxin and antitoxin systems specifically mediate intraspecies
RT competition in Bacillus subtilis biofilms.";
RL PLoS Genet. 17:e1009682-e1009682(2021).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS).
RG Joint center for structural genomics (JCSG);
RT "Crystal structure of uncharacterized protein (NP_389780.1) from Bacillus
RT subtilis at 1.30 A resolution.";
RL Submitted (MAY-2007) to the PDB data bank.
CC -!- FUNCTION: Immunity component of one of 6 LXG toxin-immunity modules in
CC this strain. They promote kin selection, mediate competition in
CC biofilms, and drive spatial segregation of different strains,
CC indicating that LXG toxins may help avoid warfare between strains in
CC biofilms. Mediates intercellular competition during biofilm formation;
CC disruption of the operon disadvantages the bacteria, but overexpression
CC of the cognate immunity protein restores growth in competition with
CC wild-type. In situ neutralizes the toxic effect of cognate toxin YobL
CC (PubMed:34280190). Neutralizes the toxic activity of cognate toxin YobL
CC upon expression in E.coli. Does not have immunity protein activity on
CC other LXG toxins (PubMed:22200572). {ECO:0000269|PubMed:22200572,
CC ECO:0000269|PubMed:34280190}.
CC -!- SUBUNIT: Interacts with cognate toxin YobL but not with non-cognate
CC putative toxin YeeF. The interaction inhibits the toxic activity of
CC YobL. {ECO:0000269|PubMed:22200572}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expressed on rich and minimal solid media likely in early
CC stationary phase; not dependent on DegSU. Not expressed in liquid LB,
CC but only under conditions that promote biofilm formation.
CC {ECO:0000269|PubMed:34280190}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the yobL-yobK operon has no visible
CC growth phenotype, however it is out-competed by wild-type cells.
CC {ECO:0000269|PubMed:34280190}.
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DR EMBL; AF027868; AAB84463.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13791.1; -; Genomic_DNA.
DR PIR; B69899; B69899.
DR RefSeq; NP_389780.1; NC_000964.3.
DR RefSeq; WP_003231326.1; NZ_JNCM01000036.1.
DR PDB; 2PRV; X-ray; 1.30 A; A/B=1-152.
DR PDBsum; 2PRV; -.
DR AlphaFoldDB; O34596; -.
DR SMR; O34596; -.
DR IntAct; O34596; 1.
DR MINT; O34596; -.
DR STRING; 224308.BSU18990; -.
DR jPOST; O34596; -.
DR PaxDb; O34596; -.
DR PRIDE; O34596; -.
DR EnsemblBacteria; CAB13791; CAB13791; BSU_18990.
DR GeneID; 939629; -.
DR KEGG; bsu:BSU18990; -.
DR PATRIC; fig|224308.179.peg.2077; -.
DR eggNOG; ENOG5032WNB; Bacteria.
DR OMA; YGSGGIC; -.
DR BioCyc; BSUB:BSU18990-MON; -.
DR EvolutionaryTrace; O34596; -.
DR Proteomes; UP000001570; Chromosome.
DR Gene3D; 3.40.1580.10; -; 1.
DR InterPro; IPR018958; Knr4/Smi1-like_dom.
DR InterPro; IPR037883; Knr4/Smi1-like_sf.
DR SMART; SM00860; SMI1_KNR4; 1.
DR SUPFAM; SSF160631; SSF160631; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome.
FT CHAIN 1..152
FT /note="Immunity protein YobK"
FT /id="PRO_0000049654"
FT HELIX 1..13
FT /evidence="ECO:0007829|PDB:2PRV"
FT HELIX 14..16
FT /evidence="ECO:0007829|PDB:2PRV"
FT HELIX 25..35
FT /evidence="ECO:0007829|PDB:2PRV"
FT HELIX 41..50
FT /evidence="ECO:0007829|PDB:2PRV"
FT STRAND 52..55
FT /evidence="ECO:0007829|PDB:2PRV"
FT STRAND 58..60
FT /evidence="ECO:0007829|PDB:2PRV"
FT STRAND 65..67
FT /evidence="ECO:0007829|PDB:2PRV"
FT HELIX 69..80
FT /evidence="ECO:0007829|PDB:2PRV"
FT STRAND 87..92
FT /evidence="ECO:0007829|PDB:2PRV"
FT STRAND 94..101
FT /evidence="ECO:0007829|PDB:2PRV"
FT STRAND 112..116
FT /evidence="ECO:0007829|PDB:2PRV"
FT TURN 117..119
FT /evidence="ECO:0007829|PDB:2PRV"
FT STRAND 120..128
FT /evidence="ECO:0007829|PDB:2PRV"
FT HELIX 129..144
FT /evidence="ECO:0007829|PDB:2PRV"
SQ SEQUENCE 152 AA; 17715 MW; BE14F20F8125A9C3 CRC64;
MIYSKVENFI NENKQNAIFT EGASHENIGR IEENLQCDLP NSYKWFLEKY GAGGLFGVLV
LGYNFDHASV VNRTNEYKEH YGLTDGLVVI EDVDYFAYCL DTNKMKDGEC PVVEWDRVIG
YQDTVADSFI EFFYNKIQEA KDDWDEDEDW DD
