ID   YOEB_ECOL6              Reviewed;          84 AA.
AC   Q8FG53;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Toxin YoeB;
DE            EC=3.1.-.-;
DE   AltName: Full=Putative endoribonuclease YoeB;
DE   AltName: Full=Putative mRNA interferase Yoeb;
GN   Name=yoeB; OrderedLocusNames=c2544;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=23055930; DOI=10.1371/journal.ppat.1002954;
RA   Norton J.P., Mulvey M.A.;
RT   "Toxin-antitoxin systems are important for niche-specific colonization and
RT   stress resistance of uropathogenic Escherichia coli.";
RL   PLoS Pathog. 8:E1002954-E1002954(2012).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. It
CC       has been proposed to be an mRNA interferase but also an inhibitor of
CC       translation initiation. Has an in vitro RNase activity and
CC       preferentially cleaves at the 3'-end of purine ribonucleotides. YefM
CC       binds to the promoter region of the yefM-yeoB operon to repress
CC       transcription, YeoB acts as a corepressor (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Forms a complex with antitoxin YefM, in which the toxin is
CC       inactive. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the tomB-hha operon causes reduced
CC       infection of the mouse bladder but not of the kidney.
CC       {ECO:0000269|PubMed:23055930}.
CC   -!- SIMILARITY: Belongs to the YoeB family. {ECO:0000305}.
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DR   EMBL; AE014075; AAN80998.1; -; Genomic_DNA.
DR   RefSeq; WP_000767822.1; NC_004431.1.
DR   AlphaFoldDB; Q8FG53; -.
DR   SMR; Q8FG53; -.
DR   STRING; 199310.c2544; -.
DR   EnsemblBacteria; AAN80998; AAN80998; c2544.
DR   KEGG; ecc:c2544; -.
DR   eggNOG; COG4115; Bacteria.
DR   HOGENOM; CLU_169492_2_2_6; -.
DR   OMA; KCRFHYD; -.
DR   BioCyc; ECOL199310:C2544-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:InterPro.
DR   Gene3D; 3.30.2310.20; -; 1.
DR   InterPro; IPR035093; RelE/ParE_toxin_dom_sf.
DR   InterPro; IPR009614; YoeB_toxin.
DR   PANTHER; PTHR38039; PTHR38039; 1.
DR   Pfam; PF06769; YoeB_toxin; 1.
DR   SUPFAM; SSF143011; SSF143011; 1.
DR   TIGRFAMs; TIGR02116; toxin_Txe_YoeB; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Repressor; RNA-binding;
KW   Toxin-antitoxin system; Transcription; Transcription regulation.
FT   CHAIN           1..84
FT                   /note="Toxin YoeB"
FT                   /id="PRO_0000216210"
FT   ACT_SITE        46
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        83
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   84 AA;  10216 MW;  30463D1C56DC2FE4 CRC64;
     MKLIWSEESW DDYLYWQETD KRIVKKINEI IKDTRRTPFE GKGKPEPLKH NLSGFWSRRI
     TEEHRLVYAV TDDSLLIAAC RYHY