ID   YOEB_SHIFL              Reviewed;          84 AA.
AC   P69349; P56605;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Toxin YoeB;
DE            EC=3.1.-.-;
DE   AltName: Full=Putative endoribonuclease YoeB;
DE   AltName: Full=Putative mRNA interferase Yoeb;
DE   AltName: Full=Toxin RelE2;
GN   Name=yoeB; Synonyms=relE2; OrderedLocusNames=SF2076, S2198.1;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
RN   [3]
RP   POSSIBLE FUNCTION.
RC   STRAIN=301 / Serotype 2a, and ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. It
CC       has been proposed to be an mRNA interferase but also an inhibitor of
CC       translation initiation. Has an in vitro RNase activity and
CC       preferentially cleaves at the 3'-end of purine ribonucleotides. YefM
CC       binds to the promoter region of the yefM-yeoB operon to repress
CC       transcription, YeoB acts as a corepressor (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Forms a complex with antitoxin YefM, in which the toxin is
CC       inactive. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the YoeB family. {ECO:0000305}.
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DR   EMBL; AE005674; AAN43616.1; -; Genomic_DNA.
DR   EMBL; AE014073; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_707909.1; NC_004337.2.
DR   RefSeq; WP_000767829.1; NZ_WPGN01000158.1.
DR   AlphaFoldDB; P69349; -.
DR   SMR; P69349; -.
DR   STRING; 198214.SF2076; -.
DR   EnsemblBacteria; AAN43616; AAN43616; SF2076.
DR   GeneID; 1025103; -.
DR   GeneID; 66674086; -.
DR   KEGG; sfl:SF2076; -.
DR   PATRIC; fig|198214.7.peg.2486; -.
DR   HOGENOM; CLU_169492_2_2_6; -.
DR   OMA; KCRFHYD; -.
DR   OrthoDB; 2036672at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:InterPro.
DR   Gene3D; 3.30.2310.20; -; 1.
DR   InterPro; IPR035093; RelE/ParE_toxin_dom_sf.
DR   InterPro; IPR009614; YoeB_toxin.
DR   PANTHER; PTHR38039; PTHR38039; 1.
DR   Pfam; PF06769; YoeB_toxin; 1.
DR   SUPFAM; SSF143011; SSF143011; 1.
DR   TIGRFAMs; TIGR02116; toxin_Txe_YoeB; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; Repressor;
KW   RNA-binding; Toxin-antitoxin system; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..84
FT                   /note="Toxin YoeB"
FT                   /id="PRO_0000216211"
FT   ACT_SITE        46
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        83
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   84 AA;  10216 MW;  C043381C56DC2939 CRC64;
     MKLIWSEESW DDYLYWQETD KRIVKKINEL IKDTRRTPFE GKGKPEPLKH NLSGFWSRRI
     TEEHRLVYAV TDDSLLIAAC RYHY