YOHF_ECOLI
ID YOHF_ECOLI Reviewed; 253 AA.
AC P33368; P33367; P76437; Q2MAU2;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Uncharacterized oxidoreductase YohF;
DE EC=1.-.-.-;
GN Name=yohF; Synonyms=yohE; OrderedLocusNames=b2137, JW2125;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA60499.1; Type=Frameshift; Note=Produces two separate ORFs.; Evidence={ECO:0000305};
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DR EMBL; U00007; AAA60500.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U00007; AAA60499.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U00096; AAC75198.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76614.1; -; Genomic_DNA.
DR PIR; H64981; H64981.
DR RefSeq; NP_416641.1; NC_000913.3.
DR RefSeq; WP_000079538.1; NZ_LN832404.1.
DR PDB; 6OZ7; X-ray; 1.36 A; A/B/C/D/E/F/G/H=2-253.
DR PDBsum; 6OZ7; -.
DR AlphaFoldDB; P33368; -.
DR SMR; P33368; -.
DR BioGRID; 4260454; 17.
DR DIP; DIP-12806N; -.
DR IntAct; P33368; 6.
DR STRING; 511145.b2137; -.
DR jPOST; P33368; -.
DR PaxDb; P33368; -.
DR PRIDE; P33368; -.
DR EnsemblBacteria; AAC75198; AAC75198; b2137.
DR EnsemblBacteria; BAE76614; BAE76614; BAE76614.
DR GeneID; 949126; -.
DR KEGG; ecj:JW2125; -.
DR KEGG; eco:b2137; -.
DR PATRIC; fig|1411691.4.peg.105; -.
DR EchoBASE; EB1955; -.
DR eggNOG; COG1028; Bacteria.
DR HOGENOM; CLU_010194_1_3_6; -.
DR InParanoid; P33368; -.
DR OMA; GICEFKE; -.
DR PhylomeDB; P33368; -.
DR BioCyc; EcoCyc:EG12019-MON; -.
DR PRO; PR:P33368; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Oxidoreductase; Reference proteome.
FT CHAIN 1..253
FT /note="Uncharacterized oxidoreductase YohF"
FT /id="PRO_0000054840"
FT ACT_SITE 153
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 6..30
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 140
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT STRAND 4..9
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 14..24
FT /evidence="ECO:0007829|PDB:6OZ7"
FT STRAND 28..35
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 37..48
FT /evidence="ECO:0007829|PDB:6OZ7"
FT TURN 49..51
FT /evidence="ECO:0007829|PDB:6OZ7"
FT STRAND 54..58
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 61..63
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 66..69
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 70..78
FT /evidence="ECO:0007829|PDB:6OZ7"
FT STRAND 82..86
FT /evidence="ECO:0007829|PDB:6OZ7"
FT TURN 96..98
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 101..111
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 113..129
FT /evidence="ECO:0007829|PDB:6OZ7"
FT STRAND 133..138
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 141..143
FT /evidence="ECO:0007829|PDB:6OZ7"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 151..171
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 172..174
FT /evidence="ECO:0007829|PDB:6OZ7"
FT STRAND 176..183
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 214..224
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 227..229
FT /evidence="ECO:0007829|PDB:6OZ7"
FT STRAND 236..240
FT /evidence="ECO:0007829|PDB:6OZ7"
FT HELIX 243..245
FT /evidence="ECO:0007829|PDB:6OZ7"
FT TURN 248..250
FT /evidence="ECO:0007829|PDB:6OZ7"
SQ SEQUENCE 253 AA; 26951 MW; B030F9EAD7E8F42A CRC64;
MAQVAIITAS DSGIGKECAL LLAQQGFDIG ITWHSDEEGA KDTAREVVSH GVRAEIVQLD
LGNLPEGALA LEKLIQRLGR IDVLVNNAGA MTKAPFLDMA FDEWRKIFTV DVDGAFLCSQ
IAARQMVKQG QGGRIINITS VHEHTPLPDA SAYTAAKHAL GGLTKAMALE LVRHKILVNA
VAPGAIATPM NGMDDSDVKP DAEPSIPLRR FGATHEIASL VVWLCSEGAN YTTGQSLIVD
GGFMLANPQF NPE
