ID   YOKJ_BACSU              Reviewed;         165 AA.
AC   O31997;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Immunity protein YokJ;
DE   AltName: Full=SPbeta prophage-derived uncharacterized YokJ;
GN   Name=yokJ; OrderedLocusNames=BSU21570;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   FUNCTION AS AN IMMUNITY PROTEIN, AND EXPRESSION IN E.COLI.
RC   STRAIN=168;
RX   PubMed=22200572; DOI=10.1016/j.febslet.2011.12.020;
RA   Holberger L.E., Garza-Sanchez F., Lamoureux J., Low D.A., Hayes C.S.;
RT   "A novel family of toxin/antitoxin proteins in Bacillus species.";
RL   FEBS Lett. 586:132-136(2012).
RN   [3]
RP   FUNCTION AS AN IMMUNITY PROTEIN, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=34280190; DOI=10.1371/journal.pgen.1009682;
RA   Kobayashi K.;
RT   "Diverse LXG toxin and antitoxin systems specifically mediate intraspecies
RT   competition in Bacillus subtilis biofilms.";
RL   PLoS Genet. 17:e1009682-e1009682(2021).
CC   -!- FUNCTION: Immunity component of one of 6 LXG toxin-immunity modules in
CC       this strain. They promote kin selection, mediate competition in
CC       biofilms, and drive spatial segregation of different strains,
CC       indicating that LXG toxins may help avoid warfare between strains in
CC       biofilms. Mediates intercellular competition during biofilm formation;
CC       disruption of the operon disadvantages the bacteria, but overexpression
CC       of the cognate immunity protein restores growth in competition with
CC       wild-type. In situ neutralizes the toxic effect of cognate toxin YokI
CC       (PubMed:34280190). Neutralizes the ability to inhibit growth of cognate
CC       toxin YokI upon expression in E.coli. Does not have immunity protein
CC       activity on other LXG toxins (PubMed:22200572).
CC       {ECO:0000269|PubMed:22200572, ECO:0000269|PubMed:34280190}.
CC   -!- SUBUNIT: Probably interacts with cognate toxin YokI but not with other
CC       non-cognate toxins. The interaction inhibits the toxic activity of YokI
CC       (Probable). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Expressed on rich and minimal solid media likely in early
CC       stationary phase; not dependent on DegSU. Not expressed in liquid LB,
CC       but only under conditions that promote biofilm formation.
CC       {ECO:0000269|PubMed:34280190}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the yokI-yokJ operon has no visible
CC       growth phenotype, however it is out-competed by wild-type cells.
CC       {ECO:0000269|PubMed:34280190}.
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DR   EMBL; AL009126; CAB14075.1; -; Genomic_DNA.
DR   RefSeq; NP_390040.1; NC_000964.3.
DR   RefSeq; WP_003246188.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; O31997; -.
DR   STRING; 224308.BSU21570; -.
DR   jPOST; O31997; -.
DR   PaxDb; O31997; -.
DR   PRIDE; O31997; -.
DR   EnsemblBacteria; CAB14075; CAB14075; BSU_21570.
DR   GeneID; 939114; -.
DR   KEGG; bsu:BSU21570; -.
DR   PATRIC; fig|224308.179.peg.2355; -.
DR   eggNOG; ENOG502ZAX0; Bacteria.
DR   OMA; YDSFFDR; -.
DR   BioCyc; BSUB:BSU21570-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   Gene3D; 3.40.1580.10; -; 1.
DR   InterPro; IPR018958; Knr4/Smi1-like_dom.
DR   InterPro; IPR037883; Knr4/Smi1-like_sf.
DR   Pfam; PF09346; SMI1_KNR4; 1.
DR   SMART; SM00860; SMI1_KNR4; 1.
DR   SUPFAM; SSF160631; SSF160631; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..165
FT                   /note="Immunity protein YokJ"
FT                   /id="PRO_0000360584"
SQ   SEQUENCE   165 AA;  18907 MW;  8A2A06C6C24F21B1 CRC64;
     MSIDMLIKKI ASTSDCRLFE ADGLPVIDEK HQLPKDISEF YEQCGGAVLY ENADYPIYIV
     RPAEFELANP IIVGELCEED ISSEWYIVCT DGKGEYLTID LNDQRKGKCY DSFFDRHGIV
     GETQVIASSF TDLIQRLLEN KGKHWYWLRD DYVSLGDAYD GIEIE