ID   YOS1_YEAST              Reviewed;          85 AA.
AC   Q3E834; D3DLY0;
DT   18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Protein transport protein YOS1;
DE   AltName: Full=YIP one suppressor protein 1;
GN   Name=YOS1; OrderedLocusNames=YER074W-A;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169868;
RA   Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA   Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA   Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA   Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA   Botstein D., Davis R.W.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL   Nature 387:78-81(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION, INTERACTION WITH YIF1 AND YIP1, SUBCELLULAR LOCATION, AND
RP   MUTAGENESIS OF ASN-18 AND PRO-69.
RX   PubMed=15659647; DOI=10.1091/mbc.e04-10-0873;
RA   Heidtman M., Chen C.Z., Collins R.N., Barlowe C.;
RT   "Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for
RT   transport between the endoplasmic reticulum and the Golgi complex.";
RL   Mol. Biol. Cell 16:1673-1683(2005).
CC   -!- FUNCTION: Required for protein transport between endoplasmic reticulum
CC       and Golgi apparatus. {ECO:0000269|PubMed:15659647}.
CC   -!- SUBUNIT: Component of the YIP1-YIF1 complex, composed of at least YIF1,
CC       YIP1 and YOS1.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:15659647}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:15659647}. Golgi apparatus membrane
CC       {ECO:0000269|PubMed:15659647}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:15659647}.
CC   -!- SIMILARITY: Belongs to the YOS1 family. {ECO:0000305}.
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DR   EMBL; U18814; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BK006939; DAA07734.1; -; Genomic_DNA.
DR   RefSeq; NP_219496.2; NM_001184512.1.
DR   AlphaFoldDB; Q3E834; -.
DR   SMR; Q3E834; -.
DR   BioGRID; 36818; 4.
DR   STRING; 4932.YER074W-A; -.
DR   iPTMnet; Q3E834; -.
DR   PaxDb; Q3E834; -.
DR   PRIDE; Q3E834; -.
DR   TopDownProteomics; Q3E834; -.
DR   EnsemblFungi; YER074W-A_mRNA; YER074W-A; YER074W-A.
DR   GeneID; 856806; -.
DR   KEGG; sce:YER074W-A; -.
DR   SGD; S000007651; YOS1.
DR   VEuPathDB; FungiDB:YER074W-A; -.
DR   eggNOG; KOG4779; Eukaryota.
DR   GeneTree; ENSGT00510000047648; -.
DR   HOGENOM; CLU_152125_0_0_1; -.
DR   InParanoid; Q3E834; -.
DR   OMA; WASWQNV; -.
DR   BioCyc; YEAST:G3O-30392-MON; -.
DR   PRO; PR:Q3E834; -.
DR   Proteomes; UP000002311; Chromosome V.
DR   RNAct; Q3E834; protein.
DR   GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IDA:SGD.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
DR   GO; GO:0030173; C:integral component of Golgi membrane; IDA:SGD.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IMP:SGD.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR013880; Yos1.
DR   PANTHER; PTHR15858; PTHR15858; 1.
DR   Pfam; PF08571; Yos1; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Membrane;
KW   Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..85
FT                   /note="Protein transport protein YOS1"
FT                   /id="PRO_0000232636"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         18
FT                   /note="N->A: In YOS1-1; impairs interaction with YIP1,
FT                   blocks ER-Golgi protein transport, and causes a severe
FT                   growth defect at 37 degrees Celsius."
FT                   /evidence="ECO:0000269|PubMed:15659647"
FT   MUTAGEN         69
FT                   /note="P->A: Lethal."
FT                   /evidence="ECO:0000269|PubMed:15659647"
SQ   SEQUENCE   85 AA;  9436 MW;  7AE4E85AD2B43787 CRC64;
     MVLFGLGRLF YVILLLINAV AVLSEERFLR RIGLGRSNDE TPVFGQDQNT TKSKVVQLIG
     AVQTLLRIPL IGINILVIVY ELLLG