YOX1_SCHPO
ID YOX1_SCHPO Reviewed; 201 AA.
AC P40923;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=MBF complex negative regulatory component yox1;
GN Name=yox1; ORFNames=SPBC21B10.13c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8005432; DOI=10.1093/genetics/136.3.769;
RA Lin Y., Smith G.R.;
RT "Transient, meiosis-induced expression of the rec6 and rec12 genes of
RT Schizosaccharomyces pombe.";
RL Genetics 136:769-779(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP INDUCTION, IDENTIFICATION IN THE MBF COMPLEX, SUBCELLULAR LOCATION,
RP DNA-BINDING, AND FUNCTION.
RX PubMed=19714215; DOI=10.1371/journal.pgen.1000626;
RA Aligianni S., Lackner D.H., Klier S., Rustici G., Wilhelm B.T.,
RA Marguerat S., Codlin S., Brazma A., de Bruin R.A., Bahler J.;
RT "The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-
RT dependent cell-cycle transcription to G1-S via negative feedback.";
RL PLoS Genet. 5:E1000626-E1000626(2009).
RN [5]
RP FUNCTION, INDUCTION, AND PHOSPHORYLATION.
RX PubMed=21304269; DOI=10.4161/cc.10.4.14824;
RA Purtill F.S., Whitehall S.K., Williams E.S., McInerny C.J., Sharrocks A.D.,
RA Morgan B.A.;
RT "A homeodomain transcription factor regulates the DNA replication
RT checkpoint in yeast.";
RL Cell Cycle 10:664-670(2011).
CC -!- FUNCTION: Negative regulatory component of the MBF transcription factor
CC complex involved in cell-cycle G1/S phase-specific gene expression and
CC more particularly DNA replication checkpoint-dependent gene expression.
CC {ECO:0000269|PubMed:19714215, ECO:0000269|PubMed:21304269}.
CC -!- SUBUNIT: Component of the MBF transcription factor complex.
CC {ECO:0000269|PubMed:19714215}.
CC -!- INTERACTION:
CC P40923; P01129: cdc10; NbExp=4; IntAct=EBI-8530485, EBI-1009350;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:16823372, ECO:0000269|PubMed:19714215}.
CC -!- INDUCTION: Expression is induced during S phase and depends on the MBF
CC transcription factor complex. {ECO:0000269|PubMed:19714215,
CC ECO:0000269|PubMed:21304269}.
CC -!- PTM: Phosphorylated in response to hydroxyurea. Phosphorylation
CC inhibits the repressor activity and is dependent on rad3. However, the
CC regulation of yox1 by rad3 is probably indirect.
CC {ECO:0000269|PubMed:21304269}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L14773; AAA53085.1; -; Genomic_DNA.
DR EMBL; CU329671; CAB57917.1; -; Genomic_DNA.
DR PIR; S50010; S50010.
DR RefSeq; NP_595674.1; NM_001021569.2.
DR AlphaFoldDB; P40923; -.
DR SMR; P40923; -.
DR BioGRID; 276839; 83.
DR IntAct; P40923; 1.
DR MINT; P40923; -.
DR STRING; 4896.SPBC21B10.13c.1; -.
DR MaxQB; P40923; -.
DR PaxDb; P40923; -.
DR PRIDE; P40923; -.
DR EnsemblFungi; SPBC21B10.13c.1; SPBC21B10.13c.1:pep; SPBC21B10.13c.
DR GeneID; 2540309; -.
DR KEGG; spo:SPBC21B10.13c; -.
DR PomBase; SPBC21B10.13c; yox1.
DR VEuPathDB; FungiDB:SPBC21B10.13c; -.
DR eggNOG; KOG0490; Eukaryota.
DR HOGENOM; CLU_1343950_0_0_1; -.
DR InParanoid; P40923; -.
DR OMA; LCESQNG; -.
DR PRO; PR:P40923; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IPI:PomBase.
DR GO; GO:0003677; F:DNA binding; ISM:PomBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; EXP:PomBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:PomBase.
DR GO; GO:0071930; P:negative regulation of transcription involved in G1/S transition of mitotic cell cycle; IMP:PomBase.
DR GO; GO:0071931; P:positive regulation of transcription involved in G1/S transition of mitotic cell cycle; IMP:PomBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; DNA-binding; Homeobox; Nucleus; Phosphoprotein;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..201
FT /note="MBF complex negative regulatory component yox1"
FT /id="PRO_0000049411"
FT DNA_BIND 33..92
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..42
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 201 AA; 23157 MW; A817F550A88F2872 CRC64;
MSLSDSPSKS GNTGKDLISN NEAKNHEDEE THQKKRRRRT TDAEATLLEQ YFLKTPKPSL
IERQELSKKL KSSMTPRELQ IWFQNKRQSL RRSNCLSRNR LEGTGENSLL RRKSTLTLCE
TSTGQAELFF QSWPLHSQSV VGEMIHHEQD DYNKENKQQK VVDTTKDISR GSNGNEDSAA
HQELEECARS LVELQQQCND H
