YOX1_YEAST
ID YOX1_YEAST Reviewed; 385 AA.
AC P34161; D6VZE7;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Homeobox protein YOX1;
GN Name=YOX1; OrderedLocusNames=YML027W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-296.
RX PubMed=8096171; DOI=10.1007/bf00387732;
RA Kaufmann E.;
RT "In vitro binding to the leucine tRNA gene identifies a novel yeast
RT homeobox gene.";
RL Chromosoma 102:174-179(1993).
RN [5]
RP FUNCTION, DNA-BINDING, DEVELOPMENTAL STAGE, AND INTERACTION WITH MCM1.
RX PubMed=12464633; DOI=10.1101/gad.1034302;
RA Pramila T., Miles S., GuhaThakurta D., Jemiolo D., Breeden L.L.;
RT "Conserved homeodomain proteins interact with MADS box protein Mcm1 to
RT restrict ECB-dependent transcription to the M/G1 phase of the cell cycle.";
RL Genes Dev. 16:3034-3045(2002).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Transcriptional repressor required to restrict transcription
CC of ECB-dependent genes to the G1/M phase by repressing their
CC transcription during the interval from late G1 to M phases. Genes that
CC contain a ECB (early cell box) element in their transcription
CC regulatory region are transcribed only during G1/M phases. In vitro, is
CC capable of binding to the DNA of the leucine tRNA gene.
CC {ECO:0000269|PubMed:12464633}.
CC -!- SUBUNIT: Interacts with MCM1. {ECO:0000269|PubMed:12464633}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Expressed from late G1 phase.
CC {ECO:0000269|PubMed:12464633}.
CC -!- INDUCTION: Transcriptionally activated by SWI4-SWI6 heterodimer.
CC -!- MISCELLANEOUS: Present with 861 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z46659; CAA86628.1; -; Genomic_DNA.
DR EMBL; AY693100; AAT93119.1; -; Genomic_DNA.
DR EMBL; X62392; CAA44264.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09871.1; -; Genomic_DNA.
DR PIR; S49752; S49752.
DR RefSeq; NP_013685.1; NM_001182385.1.
DR AlphaFoldDB; P34161; -.
DR SMR; P34161; -.
DR BioGRID; 35142; 82.
DR DIP; DIP-4566N; -.
DR IntAct; P34161; 8.
DR MINT; P34161; -.
DR STRING; 4932.YML027W; -.
DR iPTMnet; P34161; -.
DR MaxQB; P34161; -.
DR PaxDb; P34161; -.
DR PRIDE; P34161; -.
DR EnsemblFungi; YML027W_mRNA; YML027W; YML027W.
DR GeneID; 854981; -.
DR KEGG; sce:YML027W; -.
DR SGD; S000004489; YOX1.
DR VEuPathDB; FungiDB:YML027W; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000176720; -.
DR HOGENOM; CLU_043444_0_0_1; -.
DR InParanoid; P34161; -.
DR OMA; FEICPTP; -.
DR BioCyc; YEAST:G3O-32629-MON; -.
DR PRO; PR:P34161; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P34161; protein.
DR GO; GO:0000785; C:chromatin; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; HDA:SGD.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0071930; P:negative regulation of transcription involved in G1/S transition of mitotic cell cycle; IMP:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; DNA-binding; Homeobox; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..385
FT /note="Homeobox protein YOX1"
FT /id="PRO_0000049387"
FT DNA_BIND 176..235
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 87..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 235..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..385
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..37
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..250
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..353
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 291..296
FT /note="PRPHHS -> QGLIIP (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 385 AA; 42739 MW; F21A295CDD97A282 CRC64;
MSQETKMLPS LSSLLSGTEI SSSPVSPSFT NPRTSFHLDD RGTIKLPPLN TSINRPRSVE
SALRHTVTSL HENSSAYGDD MLKHTQSDSA LSSQLNSSQE TVDESHENLL LTPLNSKKRD
YSVSSKKNDI LTPLSAAKSI IIPSASKEKR RAFAFITHSQ ETFPKKEPKI DNAPLARRKR
RRTSSQELSI LQAEFEKCPA PSKEKRIELA ESCHMTEKAV QIWFQNKRQA VKRQRIATSK
STTIIQTVSP PSPPLDVHAT PLASRVKADI LRDGSSCSRS SSSSPLENTP PRPHHSLNRR
SSTPSIKRSQ ALTFHLNPQK KTLTPVKTSP NSRVNKLINS IDHSPSKAKR PVSNPSGSPK
RKRKFGFKIV DQQPLKDLDP NAFRG
