ID   YP23_SCHPO              Reviewed;         547 AA.
AC   Q8TFF9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Putative amidase PB8B6.03;
DE            EC=3.5.1.4;
GN   ORFNames=SPBPB8B6.03;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; CU329671; CAD27909.1; -; Genomic_DNA.
DR   RefSeq; NP_001018764.1; NM_001020939.2.
DR   AlphaFoldDB; Q8TFF9; -.
DR   SMR; Q8TFF9; -.
DR   STRING; 4896.SPBPB8B6.03.1; -.
DR   PaxDb; Q8TFF9; -.
DR   PRIDE; Q8TFF9; -.
DR   EnsemblFungi; SPBPB8B6.03.1; SPBPB8B6.03.1:pep; SPBPB8B6.03.
DR   GeneID; 3361182; -.
DR   KEGG; spo:SPBPB8B6.03; -.
DR   PomBase; SPBPB8B6.03; -.
DR   VEuPathDB; FungiDB:SPBPB8B6.03; -.
DR   eggNOG; KOG1212; Eukaryota.
DR   HOGENOM; CLU_009600_9_2_1; -.
DR   InParanoid; Q8TFF9; -.
DR   OMA; SMMATET; -.
DR   PhylomeDB; Q8TFF9; -.
DR   PRO; PR:Q8TFF9; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0017064; F:fatty acid amide hydrolase activity; ISO:PomBase.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009062; P:fatty acid catabolic process; ISO:PomBase.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; Reference proteome.
FT   CHAIN           1..547
FT                   /note="Putative amidase PB8B6.03"
FT                   /id="PRO_0000316203"
FT   ACT_SITE        134
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        209
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        233
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   547 AA;  60506 MW;  C17BC75A9839B3E3 CRC64;
     MDTPTWEVVA ALKRNQVLNS IPKEWRKPNI RKEMISSGYV NTYEYLNLIL PPEENAITNL
     SMLELATNIA KGNYTSYNVT KAFCHRAALA HQILNCCIEI FFDEALKKAK ELDDVFQKTS
     KVVGPFHGIP ISLKDQVDLP GKDSSIGYVS LVGKPKTEIA LLAKILQDKG AIFYVKTTVP
     MAMMAPQTVS NLHGYTYNAL NINLSSGGSS GGEGALLGSG ASCCGIGTDI GGSIRIPSCF
     QGLYALKPST GRISYLNVTN SYSGQELIPS VIGPMARSLK DIEFFTETVI ASEAWKIDSK
     LLPIPWKNQS HLKSKKLIFG VLKTDGIVKP HPPIIRALNE VVAVLEKSGY EVIEISIPFQ
     KDMLDTVVKV FSADARFEIN NESQKTGEPV VSVVKRFVSD KIFKKPITVN EWWDLGNQVY
     KIRQMFLELW NNTAIQTLSG NSIDAIIAPI WASTSFLPES KADHLYTSLF NICDCPCVVF
     PFTKVDANID LSDVSYKPLN EEDKENNDMY DPVLFDNMPV CLQVVTKKLE EEKCLAAASS
     IMDCLTN