YP247_YEAST
ID YP247_YEAST Reviewed; 523 AA.
AC Q12523; D6W3C4;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=WD repeat-containing protein YPL247C;
GN OrderedLocusNames=YPL247C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47 AND SER-65, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 799 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the WD repeat WDR68 family. {ECO:0000305}.
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DR EMBL; Z67751; CAA91597.1; -; Genomic_DNA.
DR EMBL; Z73603; CAA97968.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11190.1; -; Genomic_DNA.
DR PIR; S61017; S61017.
DR RefSeq; NP_015077.1; NM_001184061.1.
DR AlphaFoldDB; Q12523; -.
DR SMR; Q12523; -.
DR BioGRID; 35916; 43.
DR DIP; DIP-6366N; -.
DR IntAct; Q12523; 6.
DR MINT; Q12523; -.
DR STRING; 4932.YPL247C; -.
DR iPTMnet; Q12523; -.
DR MaxQB; Q12523; -.
DR PaxDb; Q12523; -.
DR PRIDE; Q12523; -.
DR EnsemblFungi; YPL247C_mRNA; YPL247C; YPL247C.
DR GeneID; 855829; -.
DR KEGG; sce:YPL247C; -.
DR SGD; S000006168; YPL247C.
DR VEuPathDB; FungiDB:YPL247C; -.
DR eggNOG; KOG0290; Eukaryota.
DR GeneTree; ENSGT00390000006939; -.
DR HOGENOM; CLU_013694_4_0_1; -.
DR InParanoid; Q12523; -.
DR OMA; INQIQWG; -.
DR BioCyc; YEAST:G3O-34133-MON; -.
DR PRO; PR:Q12523; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q12523; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR045159; DCAF7-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19919; PTHR19919; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..523
FT /note="WD repeat-containing protein YPL247C"
FT /id="PRO_0000242490"
FT REPEAT 173..213
FT /note="WD 1"
FT REPEAT 241..281
FT /note="WD 2"
FT REPEAT 285..325
FT /note="WD 3"
FT REPEAT 392..432
FT /note="WD 4"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 436..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 47
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 65
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
SQ SEQUENCE 523 AA; 57324 MW; 02CE191AB77E712C CRC64;
MDPFHNGNKR SSISFGSSQR QPYNKNNYLS GTNGPSSAAQ DQGRGPSPFG MSGNTTNGGN
SKRNSGCDLS ATYYASRSPM YSPLDFSPPV FSPNHSQLQQ ARGYAANIPV VSNLMNPSMA
SVCEYQSHYP LFGLDWSADD YVCLGSYKED SRNKLQVLHS NDLLSWESVV DADVVYPVSK
IQWVPSQLYP RKLATCSDSL RIWSVSPEER QFQEQINLSL CKYNRQHPAN PAAADDMKVI
GTFPPITSFD WNTVDTNLII SSSIDTTCIV WDLQSSHYVK TQLIAHDSEV FDVRFLTKST
QLFASCGGDG SVRVFDLRSL AHSTIIYEPP SSSVSGATAG TITPSSKGSD ALLRLEPSPY
DPNVLATFAA DSNKIIILDM RNPESPILNL QGHGSSVNGI KWHPTKRNVL LSCGDDCQVL
YWDLNSSFME INAAGSKSPS IHGTSLEDPD GDTEMTDGGA GSGLNEDPLS LNNNSKQVCK
TLETPNMMYA NKTQEINNIA WRPQRGDWFG CVSGKKFQNV RVL
