CBPC6_MOUSE
ID CBPC6_MOUSE Reviewed; 540 AA.
AC Q09LZ8; A2A979; A6PWC5; A6PWC6; Q09LZ9;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Cytosolic carboxypeptidase 6 {ECO:0000303|PubMed:21074048};
DE EC=3.4.17.- {ECO:0000269|PubMed:21074048, ECO:0000269|PubMed:25103237, ECO:0000269|PubMed:26829768, ECO:0000269|PubMed:29593216};
DE EC=3.4.17.24 {ECO:0000269|PubMed:21074048};
DE AltName: Full=ATP/GTP-binding protein-like 4;
DE AltName: Full=Protein deglutamylase CCP6 {ECO:0000305};
GN Name=Agbl4 {ECO:0000312|MGI:MGI:1918244};
GN Synonyms=Ccp6 {ECO:0000303|PubMed:17244818};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, AND
RP SUBCELLULAR LOCATION.
RC STRAIN=C57BLKS/J;
RX PubMed=17244818; DOI=10.1096/fj.06-7329com;
RA Kalinina E., Biswas R., Berezniuk I., Hermoso A., Aviles F.X.,
RA Fricker L.D.;
RT "A novel subfamily of mouse cytosolic carboxypeptidases.";
RL FASEB J. 21:836-850(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH MYLK, AND MUTAGENESIS OF
RP HIS-230 AND GLU-233.
RX PubMed=21074048; DOI=10.1016/j.cell.2010.10.014;
RA Rogowski K., van Dijk J., Magiera M.M., Bosc C., Deloulme J.C., Bosson A.,
RA Peris L., Gold N.D., Lacroix B., Grau M.B., Bec N., Larroque C.,
RA Desagher S., Holzer M., Andrieux A., Moutin M.J., Janke C.;
RT "A family of protein-deglutamylating enzymes associated with
RT neurodegeneration.";
RL Cell 143:564-578(2010).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, AND TISSUE SPECIFICITY.
RX PubMed=25103237; DOI=10.1091/mbc.e14-06-1072;
RA Tort O., Tanco S., Rocha C., Bieche I., Seixas C., Bosc C., Andrieux A.,
RA Moutin M.J., Aviles F.X., Lorenzo J., Janke C.;
RT "The cytosolic carboxypeptidases CCP2 and CCP3 catalyze posttranslational
RT removal of acidic amino acids.";
RL Mol. Biol. Cell 25:3017-3027(2014).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP HIS-230 AND GLU-233.
RX PubMed=26829768; DOI=10.1038/ni.3356;
RA Xia P., Ye B., Wang S., Zhu X., Du Y., Xiong Z., Tian Y., Fan Z.;
RT "Glutamylation of the DNA sensor cGAS regulates its binding and synthase
RT activity in antiviral immunity.";
RL Nat. Immunol. 17:369-378(2016).
RN [6]
RP FUNCTION, CATALYTIC ACTIVITY, AND DISRUPTION PHENOTYPE.
RX PubMed=29593216; DOI=10.1038/s41467-018-03008-2;
RA Ye B., Liu B., Hao L., Zhu X., Yang L., Wang S., Xia P., Du Y., Meng S.,
RA Huang G., Qin X., Wang Y., Yan X., Li C., Hao J., Zhu P., He L., Tian Y.,
RA Fan Z.;
RT "Klf4 glutamylation is required for cell reprogramming and early embryonic
RT development in mice.";
RL Nat. Commun. 9:1261-1261(2018).
CC -!- FUNCTION: Metallocarboxypeptidase that mediates protein deglutamylation
CC of tubulin and non-tubulin target proteins (PubMed:21074048,
CC PubMed:25103237, PubMed:26829768, PubMed:29593216). Catalyzes the
CC removal of polyglutamate side chains present on the gamma-carboxyl
CC group of glutamate residues within the C-terminal tail of tubulin
CC protein (PubMed:21074048, PubMed:25103237). Specifically cleaves
CC tubulin long-side-chains, while it is not able to remove the branching
CC point glutamate (PubMed:25103237). Also catalyzes the removal of
CC polyglutamate residues from the carboxy-terminus of non-tubulin
CC proteins such as MYLK (PubMed:21074048). Mediates the deglutamylation
CC of nucleotidyltransferase CGAS, leading to CGAS antiviral defense
CC response activation (PubMed:26829768). Involved in KLF4 deglutamylation
CC which promotes KLF4 proteasome-mediated degradation, thereby negatively
CC regulating cell pluripotency maintenance and embryogenesis
CC (PubMed:29593216). {ECO:0000269|PubMed:21074048,
CC ECO:0000269|PubMed:25103237, ECO:0000269|PubMed:26829768,
CC ECO:0000269|PubMed:29593216}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O
CC = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-
CC glutamate; Xref=Rhea:RHEA:60004, Rhea:RHEA-COMP:15519, Rhea:RHEA-
CC COMP:15675, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:143623;
CC Evidence={ECO:0000269|PubMed:21074048, ECO:0000269|PubMed:25103237,
CC ECO:0000269|PubMed:26829768, ECO:0000269|PubMed:29593216};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60005;
CC Evidence={ECO:0000305|PubMed:21074048, ECO:0000305|PubMed:25103237,
CC ECO:0000305|PubMed:26829768, ECO:0000305|PubMed:29593216};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] +
CC H2O = C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + L-
CC glutamate; Xref=Rhea:RHEA:63792, Rhea:RHEA-COMP:16435, Rhea:RHEA-
CC COMP:16436, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:149555,
CC ChEBI:CHEBI:149556; EC=3.4.17.24;
CC Evidence={ECO:0000269|PubMed:21074048};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63793;
CC Evidence={ECO:0000305|PubMed:21074048};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:P00730};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:P00730};
CC -!- SUBUNIT: Interacts with MYLK. {ECO:0000269|PubMed:21074048}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:17244818}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,
CC centriole {ECO:0000250|UniProtKB:Q5VU57}. Golgi apparatus
CC {ECO:0000250|UniProtKB:Q5VU57}. Cytoplasm, cytoskeleton, cilium basal
CC body {ECO:0000250|UniProtKB:Q5VU57}. Note=Colocalizes with gamma-
CC tubulin in the centrioles at interphase and dividing cells and with
CC glutamylated tubulin in basal bodies of ciliated cells.
CC {ECO:0000250|UniProtKB:Q5VU57}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=2;
CC IsoId=Q09LZ8-1; Sequence=Displayed;
CC Name=2; Synonyms=1;
CC IsoId=Q09LZ8-2; Sequence=VSP_024671, VSP_024672;
CC -!- TISSUE SPECIFICITY: Widely expressed. Expressed abundantly in testis,
CC pituitary and brain and to a lower extent in eye, stomach, adrenal and
CC kidney. In brain, expressed at low level in cerebellum as compared to
CC cortex. {ECO:0000269|PubMed:17244818, ECO:0000269|PubMed:21074048,
CC ECO:0000269|PubMed:25103237}.
CC -!- DISRUPTION PHENOTYPE: Mice are more vulnerable to DNA virus infection
CC due to impaired immune response. Knockout mice promote somatic cell
CC reprogramming and higher litter size at birth (PubMed:29593216).
CC Knockout blastocytes show enhanced development (PubMed:29593216).
CC {ECO:0000269|PubMed:26829768, ECO:0000269|PubMed:29593216}.
CC -!- SIMILARITY: Belongs to the peptidase M14 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAM13964.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAM16884.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAM16953.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAM17710.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAM17858.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAM19669.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAM21591.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAM21666.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ867039; ABI51958.1; -; mRNA.
DR EMBL; DQ867040; ABI51959.1; -; mRNA.
DR EMBL; AL627347; CAM13964.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL627183; CAM13964.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAM13964.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAM13964.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAM13964.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAM13964.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAM13964.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAM13964.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAM16884.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL627183; CAM16884.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAM16884.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAM16884.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAM16884.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAM16884.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAM16884.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAM16884.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAM16953.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL627183; CAM16953.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAM16953.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAM16953.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAM16953.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAM16953.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAM16953.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAM16953.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAM17710.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL627183; CAM17710.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAM17710.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAM17710.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAM17710.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAM17710.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAM17710.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAM17710.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAM17858.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL627183; CAM17858.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAM17858.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAM17858.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAM17858.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAM17858.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAM17858.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAM17858.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAM19669.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL627183; CAM19669.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAM19669.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAM19669.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAM19669.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAM19669.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAM19669.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAM19669.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAM21591.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL627183; CAM21591.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAM21591.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAM21591.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAM21591.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAM21591.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAM21591.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAM21591.1; JOINED; Genomic_DNA.
DR EMBL; AL627183; CAM21666.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL627347; CAM21666.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAM21666.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAM21666.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAM21666.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAM21666.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAM21666.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAM21666.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO77735.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO77735.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO77735.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO77735.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO77735.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO77735.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO77735.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO77735.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO77918.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO77918.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO77918.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO77918.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO77918.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO77918.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO77918.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO77918.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO77920.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO77920.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO77920.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO77920.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO77920.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO77920.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO77920.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO77920.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO78078.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO78078.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO78078.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO78078.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO78078.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO78078.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO78078.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO78078.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO78121.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO78121.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO78121.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO78121.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO78121.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO78121.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO78121.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO78121.1; JOINED; Genomic_DNA.
DR EMBL; AL627183; CAP19222.1; -; Genomic_DNA.
DR EMBL; AL627347; CAP19222.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAP19222.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAP19222.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAP19222.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAP19222.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAP19222.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAP19222.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAQ11261.1; -; Genomic_DNA.
DR EMBL; AL627183; CAQ11261.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAQ11261.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAQ11261.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAQ11261.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAQ11261.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAQ11261.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAQ11261.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAQ13051.1; -; Genomic_DNA.
DR EMBL; AL627183; CAQ13051.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAQ13051.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAQ13051.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAQ13051.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAQ13051.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAQ13051.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAQ13051.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO77734.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO77734.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO77734.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO77734.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO77734.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO77734.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO77734.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO77734.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO77917.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO77917.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO77917.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO77917.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO77917.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO77917.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO77917.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO77917.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO77919.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO77919.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO77919.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO77919.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO77919.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO77919.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO77919.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO77919.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO78077.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO78077.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO78077.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO78077.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO78077.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO78077.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO78077.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO78077.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAO78120.1; -; Genomic_DNA.
DR EMBL; AL627183; CAO78120.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAO78120.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAO78120.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAO78120.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAO78120.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAO78120.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAO78120.1; JOINED; Genomic_DNA.
DR EMBL; AL627183; CAP19221.1; -; Genomic_DNA.
DR EMBL; AL627347; CAP19221.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAP19221.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAP19221.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAP19221.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAP19221.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAP19221.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAP19221.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAQ11260.1; -; Genomic_DNA.
DR EMBL; AL627183; CAQ11260.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAQ11260.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAQ11260.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAQ11260.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAQ11260.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAQ11260.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAQ11260.1; JOINED; Genomic_DNA.
DR EMBL; AL669965; CAQ13050.1; -; Genomic_DNA.
DR EMBL; AL627183; CAQ13050.1; JOINED; Genomic_DNA.
DR EMBL; AL627347; CAQ13050.1; JOINED; Genomic_DNA.
DR EMBL; AL627425; CAQ13050.1; JOINED; Genomic_DNA.
DR EMBL; AL662829; CAQ13050.1; JOINED; Genomic_DNA.
DR EMBL; AL669940; CAQ13050.1; JOINED; Genomic_DNA.
DR EMBL; AL669959; CAQ13050.1; JOINED; Genomic_DNA.
DR EMBL; AL671895; CAQ13050.1; JOINED; Genomic_DNA.
DR CCDS; CCDS51263.1; -. [Q09LZ8-2]
DR CCDS; CCDS51264.1; -. [Q09LZ8-1]
DR RefSeq; NP_001041654.2; NM_001048189.4. [Q09LZ8-2]
DR RefSeq; NP_084507.1; NM_030231.3. [Q09LZ8-1]
DR AlphaFoldDB; Q09LZ8; -.
DR SMR; Q09LZ8; -.
DR STRING; 10090.ENSMUSP00000095533; -.
DR MEROPS; M14.A32; -.
DR iPTMnet; Q09LZ8; -.
DR PhosphoSitePlus; Q09LZ8; -.
DR PaxDb; Q09LZ8; -.
DR PRIDE; Q09LZ8; -.
DR ProteomicsDB; 283704; -. [Q09LZ8-1]
DR ProteomicsDB; 283705; -. [Q09LZ8-2]
DR Antibodypedia; 52065; 65 antibodies from 19 providers.
DR Ensembl; ENSMUST00000080744; ENSMUSP00000079568; ENSMUSG00000061298. [Q09LZ8-2]
DR Ensembl; ENSMUST00000097920; ENSMUSP00000095533; ENSMUSG00000061298. [Q09LZ8-1]
DR GeneID; 78933; -.
DR KEGG; mmu:78933; -.
DR UCSC; uc008udc.2; mouse. [Q09LZ8-1]
DR UCSC; uc008udd.2; mouse. [Q09LZ8-2]
DR CTD; 84871; -.
DR MGI; MGI:1918244; Agbl4.
DR VEuPathDB; HostDB:ENSMUSG00000061298; -.
DR eggNOG; KOG3641; Eukaryota.
DR GeneTree; ENSGT00940000155042; -.
DR HOGENOM; CLU_007523_6_1_1; -.
DR InParanoid; Q09LZ8; -.
DR OrthoDB; 481670at2759; -.
DR PhylomeDB; Q09LZ8; -.
DR TreeFam; TF333009; -.
DR BioGRID-ORCS; 78933; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Agbl4; mouse.
DR PRO; PR:Q09LZ8; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q09LZ8; protein.
DR Bgee; ENSMUSG00000061298; Expressed in spermatid and 64 other tissues.
DR ExpressionAtlas; Q09LZ8; baseline and differential.
DR Genevisible; Q09LZ8; MM.
DR GO; GO:1904115; C:axon cytoplasm; IEA:GOC.
DR GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0004181; F:metallocarboxypeptidase activity; IDA:UniProtKB.
DR GO; GO:0015631; F:tubulin binding; IDA:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0098957; P:anterograde axonal transport of mitochondrion; IGI:MGI.
DR GO; GO:0098930; P:axonal transport; IGI:MGI.
DR GO; GO:0035609; P:C-terminal protein deglutamylation; IDA:UniProtKB.
DR GO; GO:0021954; P:central nervous system neuron development; IGI:MGI.
DR GO; GO:0051607; P:defense response to virus; IMP:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:MGI.
DR GO; GO:2000060; P:positive regulation of ubiquitin-dependent protein catabolic process; IMP:MGI.
DR GO; GO:0035608; P:protein deglutamylation; IMP:UniProtKB.
DR GO; GO:0035610; P:protein side chain deglutamylation; IDA:UniProtKB.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0120222; P:regulation of blastocyst development; IMP:MGI.
DR GO; GO:0098958; P:retrograde axonal transport of mitochondrion; IGI:MGI.
DR InterPro; IPR040626; Pepdidase_M14_N.
DR InterPro; IPR000834; Peptidase_M14.
DR Pfam; PF18027; Pepdidase_M14_N; 1.
DR Pfam; PF00246; Peptidase_M14; 1.
DR SMART; SM00631; Zn_pept; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Carboxypeptidase; Cell projection; Cytoplasm;
KW Cytoskeleton; Golgi apparatus; Hydrolase; Metal-binding; Metalloprotease;
KW Protease; Reference proteome; Zinc.
FT CHAIN 1..540
FT /note="Cytosolic carboxypeptidase 6"
FT /id="PRO_0000284836"
FT ACT_SITE 280
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P00730"
FT BINDING 230
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305|PubMed:21074048"
FT BINDING 233
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305|PubMed:21074048"
FT BINDING 328
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P00730"
FT VAR_SEQ 456..463
FT /note="SKEERRLG -> FRLQRTHL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17244818"
FT /id="VSP_024671"
FT VAR_SEQ 464..540
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17244818"
FT /id="VSP_024672"
FT MUTAGEN 230
FT /note="H->S: Abolishes deglutamylase activity; when
FT associated with Q-233."
FT /evidence="ECO:0000269|PubMed:21074048,
FT ECO:0000269|PubMed:26829768"
FT MUTAGEN 233
FT /note="E->Q: Abolishes deglutamylase activity; when
FT associated with S-230."
FT /evidence="ECO:0000269|PubMed:21074048,
FT ECO:0000269|PubMed:26829768"
SQ SEQUENCE 540 AA; 62210 MW; D9549A180D906E24 CRC64;
MAERSQTAPE AGNDTGNEDA IGGNVNKYIV LPNGYSGQPK KGHLTFDACF ESGNLGRVEQ
VSDFEYDLFI RPDTCNPRFR VWFNFTVENV KELQRVIFNI VNFSKTKSLY RDGMAPMVKS
TSRPKWQRLP PKNVYYYRCP DHRKNYVMSF AFCFDREDDI YQFAYCYPYT YTRFQHYLDS
LQKKNMDYFF REQLGQSVQQ RQLDLLTITS PENLREGSEK KVIFITGRVH PGETPSSFVC
QGIIDFLVSQ HPIARVLREH LVFKIAPMLN PDGVYLGNYR CSLMGFDLNR HWLDPSPWAH
PTLHGVKQLI IKMYNDPKTS LEFYIDIHAH STMMNGFMYG NIFEDEERFQ RQSIFPKLLC
QNAEDFSYTS TSFNRDAVKA GTGRRFLGGL LDHSSYCYTL EVSFYSYIIG GTTAAVPYTE
EAYMKLGRNV ARTFLDYYRL NSLVEKIAVP MPRLRSKEER RLGWEHPSCL RAEQPLEVLG
IPMCSGKALN EHLGNDIQWH LDCGSSTLPL GLISCSPSSS ASWNDMAMSN SILLPDHSFH
