ID   YPDA_GEOSE              Reviewed;          95 AA.
AC   P21878;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Uncharacterized protein in pdhA 5'region;
DE   AltName: Full=ORF1;
DE   Flags: Fragment;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924;
RX   PubMed=2200674; DOI=10.1111/j.1432-1033.1990.tb19128.x;
RA   Hawkins C.F., Borges A., Perham R.N.;
RT   "Cloning and sequence analysis of the genes encoding the alpha and beta
RT   subunits of the E1 component of the pyruvate dehydrogenase multienzyme
RT   complex of Bacillus stearothermophilus.";
RL   Eur. J. Biochem. 191:337-346(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2253629; DOI=10.1111/j.1432-1033.1990.tb19432.x;
RA   Borges A., Hawkins C.F., Packman L.C., Perham R.N.;
RT   "Cloning and sequence analysis of the genes encoding the dihydrolipoamide
RT   acetyltransferase and dihydrolipoamide dehydrogenase components of the
RT   pyruvate dehydrogenase multienzyme complex of Bacillus
RT   stearothermophilus.";
RL   Eur. J. Biochem. 194:95-102(1990).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. Cof family.
CC       {ECO:0000305}.
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DR   EMBL; X53560; CAA37626.1; -; Genomic_DNA.
DR   PIR; S10796; S10796.
DR   AlphaFoldDB; P21878; -.
DR   SMR; P21878; -.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   PROSITE; PS01229; COF_2; 1.
PE   3: Inferred from homology;
KW   Magnesium; Metal-binding.
FT   CHAIN           <1..95
FT                   /note="Uncharacterized protein in pdhA 5'region"
FT                   /id="PRO_0000054444"
FT   BINDING         21
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
FT   BINDING         44
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         47
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   95 AA;  10441 MW;  811E9487321A74E3 CRC64;
     EFRFVRWHDV STDVLPAGGS KAEGIRLMIE KLGIDKGDVY AFGDGLNDIE MLSFVGTGVA
     MGNAHEEVKR VADFVTKPVS EEGIWHGLKQ LQLIP