YPDB_ECOLI
ID YPDB_ECOLI Reviewed; 244 AA.
AC P0AE39; P77742;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Transcriptional regulatory protein YpdB {ECO:0000305};
GN Name=ypdB; OrderedLocusNames=b2381, JW2378;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, DNA-BINDING, AND MUTAGENESIS OF ASP-53.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=23222720; DOI=10.1128/jb.02051-12;
RA Fried L., Behr S., Jung K.;
RT "Identification of a target gene and activating stimulus for the YpdA/YpdB
RT histidine kinase/response regulator system in Escherichia coli.";
RL J. Bacteriol. 195:807-815(2013).
RN [5]
RP FUNCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=24659770; DOI=10.1128/jb.01554-14;
RA Behr S., Fried L., Jung K.;
RT "Identification of a novel nutrient-sensing histidine kinase/response
RT regulator network in Escherichia coli.";
RL J. Bacteriol. 196:2023-2029(2014).
CC -!- FUNCTION: Member of the two-component regulatory system YpdA/YpdB,
CC which is part of a nutrient-sensing regulatory network composed of
CC YpdA/YpdB, the high-affinity pyruvate signaling system BtsS/BtsR and
CC their respective target proteins, YhjX and BtsT. YpdB regulates
CC expression of yhjX by binding to its promoter region. Activation of the
CC YpdA/YpdB signaling cascade also promotes BtsS/BtsR-mediated btsT
CC expression. {ECO:0000269|PubMed:23222720, ECO:0000269|PubMed:24659770}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: Phosphorylated by YpdA. {ECO:0000250}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U00096; AAC75440.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16251.1; -; Genomic_DNA.
DR PIR; B65012; B65012.
DR RefSeq; NP_416882.1; NC_000913.3.
DR RefSeq; WP_001295458.1; NZ_STEB01000008.1.
DR AlphaFoldDB; P0AE39; -.
DR SMR; P0AE39; -.
DR BioGRID; 4260560; 22.
DR DIP; DIP-48228N; -.
DR IntAct; P0AE39; 2.
DR STRING; 511145.b2381; -.
DR jPOST; P0AE39; -.
DR PaxDb; P0AE39; -.
DR PRIDE; P0AE39; -.
DR EnsemblBacteria; AAC75440; AAC75440; b2381.
DR EnsemblBacteria; BAA16251; BAA16251; BAA16251.
DR GeneID; 67416782; -.
DR GeneID; 947395; -.
DR KEGG; ecj:JW2378; -.
DR KEGG; eco:b2381; -.
DR PATRIC; fig|1411691.4.peg.4347; -.
DR EchoBASE; EB3901; -.
DR eggNOG; COG3279; Bacteria.
DR HOGENOM; CLU_000445_14_1_6; -.
DR InParanoid; P0AE39; -.
DR OMA; DIHMPDF; -.
DR PhylomeDB; P0AE39; -.
DR BioCyc; EcoCyc:G7244-MON; -.
DR PRO; PR:P0AE39; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:EcoCyc.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR007492; LytTR_DNA-bd_dom.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF04397; LytTR; 1.
DR Pfam; PF00072; Response_reg; 1.
DR SMART; SM00850; LytTR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS50930; HTH_LYTTR; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..244
FT /note="Transcriptional regulatory protein YpdB"
FT /id="PRO_0000081381"
FT DOMAIN 2..116
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 139..244
FT /note="HTH LytTR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00112"
FT MOD_RES 53
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MUTAGEN 53
FT /note="D->E: Strong induction of yhjX."
FT /evidence="ECO:0000269|PubMed:23222720"
FT MUTAGEN 53
FT /note="D->N: Prevents yhjX induction."
FT /evidence="ECO:0000269|PubMed:23222720"
SQ SEQUENCE 244 AA; 28721 MW; 679EA97A44F1260B CRC64;
MKVIIVEDEF LAQQELSWLI KEHSQMEIVG TFDDGLDVLK FLQHNRVDAI FLDINIPSLD
GVLLAQNISQ FAHKPFIVFI TAWKEHAVEA FELEAFDYIL KPYQESRITG MLQKLEAAWQ
QQQTSSTPAA TVTRENDTIN LVKDERIIVT PINDIYYAEA HEKMTFVYTR RESYVMPMNI
TEFCSKLPPS HFFRCHRSFC VNLNKIREIE PWFNNTYILR LKDLDFEVPV SRSKVKEFRQ
LMHL
