ID   YPFH_ECOLI              Reviewed;         232 AA.
AC   P76561; Q2MAI4;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Esterase YpfH;
DE            EC=3.1.-.-;
GN   Name=ypfH; OrderedLocusNames=b2473, JW5396;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   FUNCTION.
RX   PubMed=15808744; DOI=10.1016/j.fmrre.2004.12.006;
RA   Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A.,
RA   Arrowsmith C.H., Edwards A.M., Yakunin A.F.;
RT   "Enzyme genomics: application of general enzymatic screens to discover new
RT   enzymes.";
RL   FEMS Microbiol. Rev. 29:263-279(2005).
CC   -!- FUNCTION: Displays esterase activity toward palmitoyl-CoA and pNP-
CC       butyrate. {ECO:0000269|PubMed:15808744}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. AB hydrolase 2
CC       family. {ECO:0000305}.
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DR   EMBL; U00096; AAC75526.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76722.1; -; Genomic_DNA.
DR   PIR; H65022; H65022.
DR   RefSeq; NP_416968.2; NC_000913.3.
DR   RefSeq; WP_000679799.1; NZ_LN832404.1.
DR   AlphaFoldDB; P76561; -.
DR   SMR; P76561; -.
DR   BioGRID; 4259532; 4.
DR   BioGRID; 851887; 1.
DR   IntAct; P76561; 1.
DR   STRING; 511145.b2473; -.
DR   ESTHER; ecoli-ypfh; LYsophospholipase_carboxylesterase.
DR   MEROPS; S09.A43; -.
DR   jPOST; P76561; -.
DR   PaxDb; P76561; -.
DR   PRIDE; P76561; -.
DR   EnsemblBacteria; AAC75526; AAC75526; b2473.
DR   EnsemblBacteria; BAE76722; BAE76722; BAE76722.
DR   GeneID; 947571; -.
DR   KEGG; ecj:JW5396; -.
DR   KEGG; eco:b2473; -.
DR   PATRIC; fig|1411691.4.peg.4266; -.
DR   EchoBASE; EB3947; -.
DR   eggNOG; COG0400; Bacteria.
DR   HOGENOM; CLU_049413_5_2_6; -.
DR   InParanoid; P76561; -.
DR   OMA; RYTVPKH; -.
DR   PhylomeDB; P76561; -.
DR   BioCyc; EcoCyc:G7296-MON; -.
DR   BRENDA; 3.1.2.2; 2026.
DR   PRO; PR:P76561; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IDA:EcoCyc.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IDA:EcoliWiki.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR003140; PLipase/COase/thioEstase.
DR   Pfam; PF02230; Abhydrolase_2; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..232
FT                   /note="Esterase YpfH"
FT                   /id="PRO_0000102276"
FT   ACT_SITE        111
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        159
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        191
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   232 AA;  24855 MW;  E046F9D642076B41 CRC64;
     MKHDHFVVQS PDKPAQQLLL LFHGVGDNPV AMGEIGNWFA PLFPDALVVS VGGAEPSGNP
     AGRQWFSVQG ITEDNRQARV DAIMPTFIET VRYWQKQSGV GANATALIGF SQGAIMVLES
     IKAEPGLASR VIAFNGRYAS LPETASTATT IHLIHGGEDP VIDLAHAVAA QEALISAGGD
     VTLDIVEDLG HAIDNRSMQF ALDHLRYTIP KHYFDEALSG GKPGDDDVIE MM