YPT35_YEAS7
ID YPT35_YEAS7 Reviewed; 214 AA.
AC A6ZT13;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Endosomal/vacuolar adapter protein YPT35 {ECO:0000305};
DE AltName: Full=PX domain-containing protein YPT35 {ECO:0000305};
GN Name=YPT35; ORFNames=SCY_2497;
OS Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=307796;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YJM789;
RX PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA Steinmetz L.M.;
RT "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT strain YJM789.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC -!- FUNCTION: Recruits the lipid transfer protein VPS13 to endosomal and
CC vacuolar membranes. {ECO:0000250|UniProtKB:P38815}.
CC -!- SUBUNIT: Interacts with RBD2, YIF1, YIP1 and YIP4.
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250|UniProtKB:P38815};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:P38815}. Vacuole
CC membrane {ECO:0000250|UniProtKB:P38815}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P38815}.
CC -!- DOMAIN: The PX domain binds phosphatidylinositol 3-phosphate
CC (PtdIns(3)P) which is necessary for peripheral membrane localization.
CC {ECO:0000250|UniProtKB:P38815}.
CC -!- SIMILARITY: Belongs to the YPT35 family. {ECO:0000305}.
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DR EMBL; AAFW02000082; EDN62344.1; -; Genomic_DNA.
DR AlphaFoldDB; A6ZT13; -.
DR SMR; A6ZT13; -.
DR PRIDE; A6ZT13; -.
DR EnsemblFungi; EDN62344; EDN62344; SCY_2497.
DR HOGENOM; CLU_1475537_0_0_1; -.
DR Proteomes; UP000007060; Unassembled WGS sequence.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IEA:InterPro.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; ISS:UniProtKB.
DR GO; GO:0072657; P:protein localization to membrane; ISS:UniProtKB.
DR CDD; cd07280; PX_YPT35; 1.
DR Gene3D; 3.30.1520.10; -; 1.
DR InterPro; IPR001683; PX_dom.
DR InterPro; IPR036871; PX_dom_sf.
DR InterPro; IPR037917; Ypt35_PX.
DR Pfam; PF00787; PX; 1.
DR SMART; SM00312; PX; 1.
DR SUPFAM; SSF64268; SSF64268; 1.
DR PROSITE; PS50195; PX; 1.
PE 3: Inferred from homology;
KW Endosome; Membrane; Phosphoprotein; Vacuole.
FT CHAIN 1..214
FT /note="Endosomal/vacuolar adapter protein YPT35"
FT /id="PRO_0000333496"
FT DOMAIN 73..213
FT /note="PX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 5..15
FT /note="PxP"
FT /evidence="ECO:0000250|UniProtKB:P38815"
FT COMPBIAS 31..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 65
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P38815"
FT MOD_RES 66
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P38815"
SQ SEQUENCE 214 AA; 24673 MW; 1B00C38EC8A3BAFB CRC64;
MNDKISFLPP EPIQLLDEDS TEPELDIDSQ QENEGPISAS NSNDSTSHSN DCGATITRTR
PRRSSSINAN FSFQKAHVSD CTIVNGDHGT KFAVWRITVF LEPNLKAFAA KRESYKIQTY
KRYSDFVRLR ENLLTRIKTA KPEKLNCLQI PHLPPSVQWY SSWKYQEVNL NKDWLAKRQR
GLEYFLNHII LNSSLVEMTK DILIQFLEPS KRVA
