YPT53_YEAST
ID YPT53_YEAST Reviewed; 220 AA.
AC P36019; D6W187; Q45TZ5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 185.
DE RecName: Full=GTP-binding protein YPT53;
GN Name=YPT53; OrderedLocusNames=YNL093W; ORFNames=N2223;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8163546; DOI=10.1083/jcb.125.2.283;
RA Singer-Krueger B., Stenmark H., Duesterhoeft A., Philippsen P., Yoo J.-S.,
RA Gallwitz D., Zerial M.;
RT "Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the
RT endocytic and vacuolar protein sorting pathways of yeast.";
RL J. Cell Biol. 125:283-298(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 200060 / W303, S96, SK1, YJM 1129, YJM 269, YJM 270, YJM 280,
RC YJM 320, YJM 326, YJM 627, and YJM 789;
RX PubMed=11907579; DOI=10.1038/416326a;
RA Steinmetz L.M., Sinha H., Richards D.R., Spiegelman J.I., Oefner P.J.,
RA McCusker J.H., Davis R.W.;
RT "Dissecting the architecture of a quantitative trait locus in yeast.";
RL Nature 416:326-330(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SK1;
RX PubMed=16273108; DOI=10.1038/ng1674;
RA Deutschbauer A.M., Davis R.W.;
RT "Quantitative trait loci mapped to single-nucleotide resolution in yeast.";
RL Nat. Genet. 37:1333-1340(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8771715;
RX DOI=10.1002/(sici)1097-0061(199605)12:6<599::aid-yea938>3.0.co;2-9;
RA Garcia-Cantalejo J.M., Boskovic J., Jimenez A.;
RT "Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae
RT chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four
RT new open reading frames.";
RL Yeast 12:599-608(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for transport in the endocytic pathway and for
CC correct sorting of the vacuolar hydrolases suggesting a possible
CC intersection of the endocytic with the vacuolar sorting pathway.
CC -!- INTERACTION:
CC P36019; P53845: YIF1; NbExp=2; IntAct=EBI-29415, EBI-28230;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 830 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; X76175; CAA53771.1; -; Genomic_DNA.
DR EMBL; AF458969; AAM00516.1; -; Genomic_DNA.
DR EMBL; AF458970; AAM00522.1; -; Genomic_DNA.
DR EMBL; AF458971; AAM00528.1; -; Genomic_DNA.
DR EMBL; AF458972; AAM00534.1; -; Genomic_DNA.
DR EMBL; AF458975; AAM00552.1; -; Genomic_DNA.
DR EMBL; AF458976; AAM00558.1; -; Genomic_DNA.
DR EMBL; AF458977; AAM00564.1; -; Genomic_DNA.
DR EMBL; AF458978; AAM00570.1; -; Genomic_DNA.
DR EMBL; AF458979; AAM00576.1; -; Genomic_DNA.
DR EMBL; AF458980; AAM00582.1; -; Genomic_DNA.
DR EMBL; AF458981; AAM00588.1; -; Genomic_DNA.
DR EMBL; DQ115393; AAZ22515.1; -; Genomic_DNA.
DR EMBL; X85811; CAA59824.1; -; Genomic_DNA.
DR EMBL; Z71369; CAA95969.1; -; Genomic_DNA.
DR EMBL; AY558420; AAS56746.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10453.1; -; Genomic_DNA.
DR PIR; S45168; S45168.
DR RefSeq; NP_014306.3; NM_001182931.3.
DR AlphaFoldDB; P36019; -.
DR SMR; P36019; -.
DR BioGRID; 35731; 66.
DR DIP; DIP-2025N; -.
DR IntAct; P36019; 24.
DR MINT; P36019; -.
DR STRING; 4932.YNL093W; -.
DR MaxQB; P36019; -.
DR PaxDb; P36019; -.
DR PRIDE; P36019; -.
DR EnsemblFungi; YNL093W_mRNA; YNL093W; YNL093W.
DR GeneID; 855631; -.
DR KEGG; sce:YNL093W; -.
DR SGD; S000005037; YPT53.
DR VEuPathDB; FungiDB:YNL093W; -.
DR eggNOG; KOG0092; Eukaryota.
DR GeneTree; ENSGT00940000161833; -.
DR HOGENOM; CLU_041217_10_2_1; -.
DR InParanoid; P36019; -.
DR OMA; SESHRTC; -.
DR BioCyc; YEAST:G3O-33121-MON; -.
DR Reactome; R-SCE-8873719; RAB geranylgeranylation.
DR PRO; PR:P36019; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P36019; protein.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005770; C:late endosome; IMP:SGD.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IDA:SGD.
DR GO; GO:0006897; P:endocytosis; IMP:SGD.
DR GO; GO:0006895; P:Golgi to endosome transport; IGI:SGD.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IMP:SGD.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW Cell membrane; GTP-binding; Lipoprotein; Membrane; Methylation;
KW Nucleotide-binding; Prenylation; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..220
FT /note="GTP-binding protein YPT53"
FT /id="PRO_0000121327"
FT BINDING 19..26
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 67..71
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 125..128
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 220
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 218
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 220
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 220 AA; 24648 MW; ABA1D926D1EE9F68 CRC64;
MDKHTAAIPT LTIKVVLLGE SAVGKSSIVL RFVSDDFKES KEPTIGAAFL TKRITRDGKV
IKFEIWDTAG QERFAPLAPM YYRNAQAALV VFDVTNEGSF YKAQNWVEEL HEKVGHDIVI
ALVGNKMDLL NNDDENENRA MKAPAVQNLC ERENLLYFEA SAKTGENIYQ IFQTLGEKVP
CPEQNTRQSS THDRTITDNQ RIDLESTTVE STRETGGCNC
