YQCF_BACSU
ID YQCF_BACSU Reviewed; 192 AA.
AC P45941;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Immunity protein YqcF;
GN Name=yqcF; OrderedLocusNames=BSU25870;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=7704261; DOI=10.1099/13500872-141-2-323;
RA Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.;
RT "Complete nucleotide sequence of a skin element excised by DNA
RT rearrangement during sporulation in Bacillus subtilis.";
RL Microbiology 141:323-327(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP IDENTIFICATION.
RX PubMed=7489895; DOI=10.1016/0378-1119(95)00636-k;
RA Medigue C., Moszer I., Viari A., Danchin A.;
RT "Analysis of a Bacillus subtilis genome fragment using a co-operative
RT computer system prototype.";
RL Gene 165:GC37-GC51(1995).
RN [5]
RP FUNCTION AS AN IMMUNITY PROTEIN, AND EXPRESSION IN E.COLI.
RC STRAIN=168;
RX PubMed=22200572; DOI=10.1016/j.febslet.2011.12.020;
RA Holberger L.E., Garza-Sanchez F., Lamoureux J., Low D.A., Hayes C.S.;
RT "A novel family of toxin/antitoxin proteins in Bacillus species.";
RL FEBS Lett. 586:132-136(2012).
RN [6]
RP FUNCTION AS AN IMMUNITY PROTEIN, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=34280190; DOI=10.1371/journal.pgen.1009682;
RA Kobayashi K.;
RT "Diverse LXG toxin and antitoxin systems specifically mediate intraspecies
RT competition in Bacillus subtilis biofilms.";
RL PLoS Genet. 17:e1009682-e1009682(2021).
CC -!- FUNCTION: Immunity component of one of 6 LXG toxin-immunity modules in
CC this strain. They promote kin selection, mediate competition in
CC biofilms, and drive spatial segregation of different strains,
CC indicating that LXG toxins may help avoid warfare between strains in
CC biofilms. Mediates intercellular competition during biofilm formation;
CC disruption of the operon disadvantages the bacteria, but overexpression
CC of the cognate immunity protein restores growth in competition with
CC wild-type. In situ neutralizes the toxic effect of cognate toxin YqcG
CC (PubMed:34280190). Neutralizes the toxic activity of cognate toxin YqcG
CC upon expression in E.coli. Does not have immunity protein activity on
CC other LXG toxins (PubMed:22200572). {ECO:0000269|PubMed:22200572,
CC ECO:0000269|PubMed:34280190}.
CC -!- SUBUNIT: Probably interacts with cognate toxin YqcG but not with other
CC non-cognate toxins. The interaction inhibits the toxic activity of YqcG
CC (Probable). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expressed on rich and minimal solid media likely in early
CC stationary phase; dependent on DegSU. Not expressed in liquid LB, but
CC only under conditions that promote biofilm formation.
CC {ECO:0000269|PubMed:34280190}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the yqcF-yqcG operon has no visible
CC growth phenotype, however it is out-competed by wild-type cells.
CC {ECO:0000269|PubMed:34280190}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D32216; BAA06963.1; -; Genomic_DNA.
DR EMBL; D84432; BAA12427.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14528.1; -; Genomic_DNA.
DR PIR; E69949; E69949.
DR RefSeq; NP_390464.1; NC_000964.3.
DR RefSeq; WP_009967790.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P45941; -.
DR SMR; P45941; -.
DR STRING; 224308.BSU25870; -.
DR PaxDb; P45941; -.
DR PRIDE; P45941; -.
DR EnsemblBacteria; CAB14528; CAB14528; BSU_25870.
DR GeneID; 937785; -.
DR KEGG; bsu:BSU25870; -.
DR PATRIC; fig|224308.179.peg.2811; -.
DR eggNOG; ENOG5032REF; Bacteria.
DR InParanoid; P45941; -.
DR OMA; LANCIFH; -.
DR BioCyc; BSUB:BSU25870-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR InterPro; IPR020941; SUFU-like_domain.
DR Pfam; PF05076; SUFU; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome.
FT CHAIN 1..192
FT /note="Immunity protein YqcF"
FT /id="PRO_0000049776"
SQ SEQUENCE 192 AA; 21476 MW; 56210B4C7405BBB8 CRC64;
MGVTQENKVI ARTVLGAFGG KPKVTKYWDD NKNSSIDILS VSDQPQEGIT SYSTLGLSDH
SINYEVNGTP LRIEIVAAME SASDIYANVL STCAFNIINS NFTCAPGVIF KNVISMYDQE
TDMKHIMFVP PFLWEEDLEL LEFSNKNVTW LMALPISEGE LQVAEKHGSD YLQDLLESKQ
IDIFDIKRES VV
