YQGF_BACSU
ID YQGF_BACSU Reviewed; 138 AA.
AC O34634;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Putative pre-16S rRNA nuclease {ECO:0000255|HAMAP-Rule:MF_00651};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00651};
GN Name=yrrK; Synonyms=yqgf {ECO:0000303|PubMed:16021622};
GN OrderedLocusNames=BSU27390;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=12682299; DOI=10.1073/pnas.0730515100;
RA Kobayashi K., Ehrlich S.D., Albertini A., Amati G., Andersen K.K.,
RA Arnaud M., Asai K., Ashikaga S., Aymerich S., Bessieres P., Boland F.,
RA Brignell S.C., Bron S., Bunai K., Chapuis J., Christiansen L.C.,
RA Danchin A., Debarbouille M., Dervyn E., Deuerling E., Devine K.,
RA Devine S.K., Dreesen O., Errington J., Fillinger S., Foster S.J.,
RA Fujita Y., Galizzi A., Gardan R., Eschevins C., Fukushima T., Haga K.,
RA Harwood C.R., Hecker M., Hosoya D., Hullo M.F., Kakeshita H., Karamata D.,
RA Kasahara Y., Kawamura F., Koga K., Koski P., Kuwana R., Imamura D.,
RA Ishimaru M., Ishikawa S., Ishio I., Le Coq D., Masson A., Mauel C.,
RA Meima R., Mellado R.P., Moir A., Moriya S., Nagakawa E., Nanamiya H.,
RA Nakai S., Nygaard P., Ogura M., Ohanan T., O'Reilly M., O'Rourke M.,
RA Pragai Z., Pooley H.M., Rapoport G., Rawlins J.P., Rivas L.A., Rivolta C.,
RA Sadaie A., Sadaie Y., Sarvas M., Sato T., Saxild H.H., Scanlan E.,
RA Schumann W., Seegers J.F., Sekiguchi J., Sekowska A., Seror S.J., Simon M.,
RA Stragier P., Studer R., Takamatsu H., Tanaka T., Takeuchi M.,
RA Thomaides H.B., Vagner V., van Dijl J.M., Watabe K., Wipat A., Yamamoto H.,
RA Yamamoto M., Yamamoto Y., Yamane K., Yata K., Yoshida K., Yoshikawa H.,
RA Zuber U., Ogasawara N.;
RT "Essential Bacillus subtilis genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:4678-4683(2003).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) OF 2-138, AND DISCUSSION OF POSSIBLE
RP FUNCTION.
RX PubMed=16021622; DOI=10.1002/prot.20541;
RA Badger J., Sauder J.M., Adams J.M., Antonysamy S., Bain K., Bergseid M.G.,
RA Buchanan S.G., Buchanan M.D., Batiyenko Y., Christopher J.A., Emtage S.,
RA Eroshkina A., Feil I., Furlong E.B., Gajiwala K.S., Gao X., He D.,
RA Hendle J., Huber A., Hoda K., Kearins P., Kissinger C., Laubert B.,
RA Lewis H.A., Lin J., Loomis K., Lorimer D., Louie G., Maletic M.,
RA Marsh C.D., Miller I., Molinari J., Muller-Dieckmann H.J., Newman J.M.,
RA Noland B.W., Pagarigan B., Park F., Peat T.S., Post K.W., Radojicic S.,
RA Ramos A., Romero R., Rutter M.E., Sanderson W.E., Schwinn K.D., Tresser J.,
RA Winhoven J., Wright T.A., Wu L., Xu J., Harris T.J.R.;
RT "Structural analysis of a set of proteins resulting from a bacterial
RT genomics project.";
RL Proteins 60:787-796(2005).
CC -!- FUNCTION: Could be a nuclease involved in processing of the 5'-end of
CC pre-16S rRNA. {ECO:0000255|HAMAP-Rule:MF_00651}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00651}.
CC -!- DISRUPTION PHENOTYPE: Not essential, it can be disrupted.
CC {ECO:0000269|PubMed:12682299}.
CC -!- SIMILARITY: Belongs to the YqgF nuclease family. {ECO:0000255|HAMAP-
CC Rule:MF_00651}.
CC -!- CAUTION: Was originally suggested to be a nuclease that resolves
CC Holliday junction intermediates during genetic recombination.
CC {ECO:0000303|PubMed:16021622}.
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DR EMBL; AL009126; CAB14681.1; -; Genomic_DNA.
DR PIR; D69979; D69979.
DR RefSeq; NP_390617.1; NC_000964.3.
DR RefSeq; WP_003229795.1; NZ_JNCM01000036.1.
DR PDB; 1VHX; X-ray; 1.96 A; A/B=2-138.
DR PDBsum; 1VHX; -.
DR AlphaFoldDB; O34634; -.
DR SMR; O34634; -.
DR STRING; 224308.BSU27390; -.
DR PaxDb; O34634; -.
DR PRIDE; O34634; -.
DR EnsemblBacteria; CAB14681; CAB14681; BSU_27390.
DR GeneID; 937357; -.
DR KEGG; bsu:BSU27390; -.
DR PATRIC; fig|224308.179.peg.2975; -.
DR eggNOG; COG0816; Bacteria.
DR InParanoid; O34634; -.
DR OMA; PMGWTAQ; -.
DR PhylomeDB; O34634; -.
DR BioCyc; BSUB:BSU27390-MON; -.
DR EvolutionaryTrace; O34634; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0000967; P:rRNA 5'-end processing; IBA:GO_Central.
DR CDD; cd16964; YqgF; 1.
DR Gene3D; 3.30.420.140; -; 1.
DR HAMAP; MF_00651; Nuclease_YqgF; 1.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR005227; YqgF.
DR InterPro; IPR006641; YqgF/RNaseH-like_dom.
DR InterPro; IPR037027; YqgF/RNaseH-like_dom_sf.
DR PANTHER; PTHR33317; PTHR33317; 1.
DR Pfam; PF03652; RuvX; 1.
DR SMART; SM00732; YqgFc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00250; RNAse_H_YqgF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Nuclease; Reference proteome;
KW Ribosome biogenesis.
FT CHAIN 1..138
FT /note="Putative pre-16S rRNA nuclease"
FT /id="PRO_0000172023"
FT STRAND 2..8
FT /evidence="ECO:0007829|PDB:1VHX"
FT STRAND 10..18
FT /evidence="ECO:0007829|PDB:1VHX"
FT STRAND 22..32
FT /evidence="ECO:0007829|PDB:1VHX"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:1VHX"
FT HELIX 42..49
FT /evidence="ECO:0007829|PDB:1VHX"
FT STRAND 52..60
FT /evidence="ECO:0007829|PDB:1VHX"
FT HELIX 71..87
FT /evidence="ECO:0007829|PDB:1VHX"
FT STRAND 91..94
FT /evidence="ECO:0007829|PDB:1VHX"
FT HELIX 100..109
FT /evidence="ECO:0007829|PDB:1VHX"
FT HELIX 114..137
FT /evidence="ECO:0007829|PDB:1VHX"
SQ SEQUENCE 138 AA; 15210 MW; 39F3EA8A6B3B1C06 CRC64;
MRILGLDLGT KTLGVALSDE MGWTAQGIET IKINEAEGDY GLSRLSELIK DYTIDKIVLG
FPKNMNGTVG PRGEASQTFA KVLETTYNVP VVLWDERLTT MAAEKMLIAA DVSRQKRKKV
IDKMAAVMIL QGYLDSLN
