ID   YQGF_HAEIN              Reviewed;         139 AA.
AC   P43981;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Putative pre-16S rRNA nuclease {ECO:0000255|HAMAP-Rule:MF_00651};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00651};
GN   OrderedLocusNames=HI_0305;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=11805338; DOI=10.1073/pnas.012602299;
RA   Akerley B.J., Rubin E.J., Novick V.L., Amaya K., Judson N., Mekalanos J.J.;
RT   "A genome-scale analysis for identification of genes required for growth or
RT   survival of Haemophilus influenzae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:966-971(2002).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=15044829; DOI=10.1159/000076741;
RA   Zalacain M., Biswas S., Ingraham K.A., Ambrad J., Bryant A., Chalker A.F.,
RA   Iordanescu S., Fan J., Fan F., Lunsford R.D., O'Dwyer K., Palmer L.M.,
RA   So C., Sylvester D., Volker C., Warren P., McDevitt D., Brown J.R.,
RA   Holmes D.J., Burnham M.K.;
RT   "A global approach to identify novel broad-spectrum antibacterial targets
RT   among proteins of unknown function.";
RL   J. Mol. Microbiol. Biotechnol. 6:109-126(2003).
CC   -!- FUNCTION: Could be a nuclease involved in processing of the 5'-end of
CC       pre-16S rRNA. {ECO:0000255|HAMAP-Rule:MF_00651}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00651}.
CC   -!- DISRUPTION PHENOTYPE: Controversial; it has been found to be non-
CC       essential (PubMed:11805338). Has also been found to be essential
CC       (PubMed:15044829). {ECO:0000269|PubMed:11805338,
CC       ECO:0000269|PubMed:15044829}.
CC   -!- SIMILARITY: Belongs to the YqgF nuclease family. {ECO:0000255|HAMAP-
CC       Rule:MF_00651}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L42023; AAC21970.1; -; Genomic_DNA.
DR   PIR; H64005; H64005.
DR   RefSeq; NP_438472.1; NC_000907.1.
DR   RefSeq; WP_005648994.1; NC_000907.1.
DR   AlphaFoldDB; P43981; -.
DR   SMR; P43981; -.
DR   STRING; 71421.HI_0305; -.
DR   EnsemblBacteria; AAC21970; AAC21970; HI_0305.
DR   KEGG; hin:HI_0305; -.
DR   PATRIC; fig|71421.8.peg.322; -.
DR   eggNOG; COG0816; Bacteria.
DR   HOGENOM; CLU_098240_3_0_6; -.
DR   OMA; PNWDDIE; -.
DR   PhylomeDB; P43981; -.
DR   BioCyc; HINF71421:G1GJ1-323-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0000967; P:rRNA 5'-end processing; IBA:GO_Central.
DR   CDD; cd16964; YqgF; 1.
DR   Gene3D; 3.30.420.140; -; 1.
DR   HAMAP; MF_00651; Nuclease_YqgF; 1.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR005227; YqgF.
DR   InterPro; IPR006641; YqgF/RNaseH-like_dom.
DR   InterPro; IPR037027; YqgF/RNaseH-like_dom_sf.
DR   PANTHER; PTHR33317; PTHR33317; 1.
DR   Pfam; PF03652; RuvX; 1.
DR   SMART; SM00732; YqgFc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   TIGRFAMs; TIGR00250; RNAse_H_YqgF; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; Nuclease; Reference proteome; Ribosome biogenesis.
FT   CHAIN           1..139
FT                   /note="Putative pre-16S rRNA nuclease"
FT                   /id="PRO_0000172071"
SQ   SEQUENCE   139 AA;  15340 MW;  A875112A732AC6B4 CRC64;
     MGITALAFDF GTKSIGCAIG QSITGTAQAL PAFKAQDGIP NWEAIEKCLK EWKPDVVIVG
     LPLNMDGTEQ DLTLRARKFA NRLQGRFGVN VHLQDERLTT TQARSEIFER GGFKALKKGK
     IDGVSACLIL ESWFEYAEY