ID   CBPR_HEVBR              Reviewed;         238 AA.
AC   Q39962;
DT   27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Citrate-binding protein;
DE   Flags: Precursor;
GN   Name=CBP;
OS   Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Micrandreae;
OC   Hevea.
OX   NCBI_TaxID=3981 {ECO:0000312|EMBL:CAA61951.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 32-50; 130-135; 137-142;
RP   161-166 AND 182-192, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. RRIM 600; TISSUE=Laticifer;
RX   PubMed=8530484; DOI=10.1074/jbc.270.51.30525;
RA   Rentsch D., Goerlach J., Vogt E., Amrhein N., Martinoia E.;
RT   "The tonoplast-associated citrate binding protein (CBP) of Hevea
RT   brasiliensis. Photoaffinity labeling, purification and cloning of the
RT   corresponding gene.";
RL   J. Biol. Chem. 270:30525-30531(1995).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 32-42; 49-60 AND 209-224.
RC   STRAIN=cv. PR 261; TISSUE=Latex;
RA   Subroto T., de Vries H., Schuringa J.J., Soedjanaatmadja U.M.S.,
RA   Hofsteenge J., Jekel P.A., Beintema J.J.;
RT   "Enzymic and structural studies on processed proteins from the vacuolar
RT   (lutoid-body) fraction of latex of Hevea brasiliensis.";
RL   Plant Physiol. Biochem. 39:1047-1055(2001).
CC   -!- FUNCTION: May be a subunit of a vacuolar malate and citrate
CC       transporter. {ECO:0000303|PubMed:8530484}.
CC   -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:8530484}. Note=Lutoid
CC       (vacuole), tonoplast.
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DR   EMBL; X89855; CAA61951.1; -; mRNA.
DR   PIR; T10760; T10760.
DR   AlphaFoldDB; Q39962; -.
DR   SMR; Q39962; -.
DR   Allergome; 1528; Hev b CitBP.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   InterPro; IPR014895; Alginate_lyase_2.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   Pfam; PF08787; Alginate_lyase2; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Signal; Vacuole.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000269|PubMed:8530484, ECO:0000269|Ref.2"
FT   CHAIN           32..224
FT                   /note="Citrate-binding protein"
FT                   /id="PRO_0000020848"
FT   PROPEP          225..238
FT                   /note="Removed in mature form"
FT                   /id="PRO_0000020849"
SQ   SEQUENCE   238 AA;  27183 MW;  4349066AC4FA18CE CRC64;
     MKMKRSPYCF CCSFALLLLV SFLKDRHFCS ADPTDGFTEV PLTEDNFVIQ KPYDKPLNDR
     YSYKNGIRRL WVYENDKPFK VGSPTRPRTE IRIKGHDYSS GVWQFEGQVH VPEGTSGVTV
     MQVFGAVNKA TALQLRVYNG DLKSYKSNSV ATDIYNKWLR VNVIHKVGKG EITVFINGQQ
     KLVVNDDGPA EHYFKCGVYA APDGSSNYME SRWKNIKLYK SDNKLEGCNN NHGTWLVQ