ID   YQGF_MYCTU              Reviewed;         170 AA.
AC   P9WGV7; L0T9Z0; P67487; P94999;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Putative pre-16S rRNA nuclease {ECO:0000255|HAMAP-Rule:MF_00651};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00651};
GN   OrderedLocusNames=Rv2554c; ORFNames=MTCY159.02;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Could be a nuclease involved in processing of the 5'-end of
CC       pre-16S rRNA. {ECO:0000255|HAMAP-Rule:MF_00651}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00651}.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC   -!- SIMILARITY: Belongs to the YqgF nuclease family. {ECO:0000255|HAMAP-
CC       Rule:MF_00651}.
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DR   EMBL; AL123456; CCP45350.1; -; Genomic_DNA.
DR   PIR; F70660; F70660.
DR   RefSeq; NP_217070.1; NC_000962.3.
DR   RefSeq; WP_003413197.1; NZ_NVQJ01000032.1.
DR   PDB; 7ESS; X-ray; 1.93 A; A/B=1-170.
DR   PDBsum; 7ESS; -.
DR   AlphaFoldDB; P9WGV7; -.
DR   SMR; P9WGV7; -.
DR   STRING; 83332.Rv2554c; -.
DR   PaxDb; P9WGV7; -.
DR   DNASU; 887490; -.
DR   GeneID; 45426557; -.
DR   GeneID; 887490; -.
DR   KEGG; mtu:Rv2554c; -.
DR   TubercuList; Rv2554c; -.
DR   eggNOG; COG0816; Bacteria.
DR   OMA; PMGWTAQ; -.
DR   PhylomeDB; P9WGV7; -.
DR   BRENDA; 3.1.21.10; 3445.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0000967; P:rRNA 5'-end processing; IBA:GO_Central.
DR   CDD; cd16964; YqgF; 1.
DR   Gene3D; 3.30.420.140; -; 1.
DR   HAMAP; MF_00651; Nuclease_YqgF; 1.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR005227; YqgF.
DR   InterPro; IPR006641; YqgF/RNaseH-like_dom.
DR   InterPro; IPR037027; YqgF/RNaseH-like_dom_sf.
DR   PANTHER; PTHR33317; PTHR33317; 1.
DR   Pfam; PF03652; RuvX; 1.
DR   SMART; SM00732; YqgFc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   TIGRFAMs; TIGR00250; RNAse_H_YqgF; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Hydrolase; Nuclease; Reference proteome;
KW   Ribosome biogenesis.
FT   CHAIN           1..170
FT                   /note="Putative pre-16S rRNA nuclease"
FT                   /id="PRO_0000172100"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..24
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   TURN            15..17
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   STRAND          24..29
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   STRAND          31..39
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   STRAND          46..53
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   HELIX           59..71
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   STRAND          74..80
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   HELIX           92..106
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   STRAND          111..115
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   HELIX           116..118
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   HELIX           121..131
FT                   /evidence="ECO:0007829|PDB:7ESS"
FT   HELIX           135..161
FT                   /evidence="ECO:0007829|PDB:7ESS"
SQ   SEQUENCE   170 AA;  18065 MW;  4418F8D396B48E20 CRC64;
     MVPAQHRPPD RPGDPAHDPG RGRRLGIDVG AARIGVACSD PDAILATPVE TVRRDRSGKH
     LRRLAALAAE LEAVEVIVGL PRTLADRIGR SAQDAIELAE ALARRVSPTP VRLADERLTT
     VSAQRSLRQA GVRASEQRAV IDQAAAVAIL QSWLDERLAA MAGTQEGSDA