YQI2_CAEEL
ID YQI2_CAEEL Reviewed; 284 AA.
AC Q09504;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Uncharacterized tRNA-dihydrouridine synthase-like protein C45G9.2;
DE EC=1.3.1.-;
GN ORFNames=C45G9.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Catalyzes the synthesis of dihydrouridine, a modified base
CC found in the D-loop of most tRNAs. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000250|UniProtKB:Q5SMC7};
CC -!- SIMILARITY: Belongs to the Dus family. {ECO:0000305}.
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DR EMBL; FO080873; CCD67391.1; -; Genomic_DNA.
DR PIR; A88449; A88449.
DR RefSeq; NP_498078.2; NM_065677.6.
DR AlphaFoldDB; Q09504; -.
DR SMR; Q09504; -.
DR STRING; 6239.C45G9.2; -.
DR EPD; Q09504; -.
DR PaxDb; Q09504; -.
DR PeptideAtlas; Q09504; -.
DR EnsemblMetazoa; C45G9.2.1; C45G9.2.1; WBGene00016674.
DR GeneID; 183479; -.
DR KEGG; cel:CELE_C45G9.2; -.
DR UCSC; C45G9.2; c. elegans.
DR CTD; 183479; -.
DR WormBase; C45G9.2; CE29722; WBGene00016674; -.
DR eggNOG; KOG2335; Eukaryota.
DR GeneTree; ENSGT00550000074907; -.
DR HOGENOM; CLU_013299_4_2_1; -.
DR InParanoid; Q09504; -.
DR OMA; LIYPYVD; -.
DR OrthoDB; 1345916at2759; -.
DR PhylomeDB; Q09504; -.
DR PRO; PR:Q09504; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00016674; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0017150; F:tRNA dihydrouridine synthase activity; IBA:GO_Central.
DR CDD; cd02801; DUS_like_FMN; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR035587; DUS-like_FMN-bd.
DR InterPro; IPR001269; DUS_fam.
DR InterPro; IPR018517; tRNA_hU_synthase_CS.
DR Pfam; PF01207; Dus; 1.
DR PIRSF; PIRSF006621; Dus; 1.
DR PROSITE; PS01136; UPF0034; 1.
PE 3: Inferred from homology;
KW Flavoprotein; FMN; Oxidoreductase; Reference proteome; tRNA processing.
FT CHAIN 1..284
FT /note="Uncharacterized tRNA-dihydrouridine synthase-like
FT protein C45G9.2"
FT /id="PRO_0000162159"
FT ACT_SITE 83
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q5SMC7"
FT BINDING 54
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:Q5SMC7"
FT BINDING 125
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:Q5SMC7"
FT BINDING 153
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:Q5SMC7"
FT BINDING 183..185
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:Q5SMC7"
FT BINDING 207..208
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:Q5SMC7"
SQ SEQUENCE 284 AA; 31839 MW; 78111AB26FE627C1 CRC64;
MVRYSKLAFR QLVRVYDVDV CFTPMIYAKN FIESEKCRSS ELSVCEGDSP LIVQFATDDP
FVLSEAAEMV YKCSTGVDLN CGCPKHDVRS KGFGSALLSK PELLADMVRQ TRARIPDPDF
SVSLKIRINH DIEKTVDLCR KAEAAGVTHL TVHGRTPSQR AEPIDIQALR IVKDSVSVPI
IANGGITTRE EALFLAEQTG VDGIMAANGL LDNPALFAGH EHTPSDCVEN FMRLSREYGL
DWLLYHQHLQ YMLRPVFSAQ QRRVFNELNG RLAIDHFLNN LLDI
