ID   YQIG_ECOLI              Reviewed;         821 AA.
AC   P76655; P77034; P77035; Q6BF51;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 3.
DT   25-MAY-2022, entry version 139.
DE   RecName: Full=Putative outer membrane usher protein YqiG;
DE   Flags: Precursor;
GN   Name=yqiG; OrderedLocusNames=b3046, JW5507;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   SEQUENCE REVISION TO 121 AND 131.
RX   PubMed=16397293; DOI=10.1093/nar/gkj405;
RA   Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA   Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA   Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA   Thomson N.R., Wishart D., Wanner B.L.;
RT   "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT   -- 2005.";
RL   Nucleic Acids Res. 34:1-9(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / BW25113;
RX   PubMed=24025676; DOI=10.1016/j.bbrc.2013.09.016;
RA   Mohd Yusoff M.Z., Hashiguchi Y., Maeda T., Wood T.K.;
RT   "Four products from Escherichia coli pseudogenes increase hydrogen
RT   production.";
RL   Biochem. Biophys. Res. Commun. 439:576-579(2013).
CC   -!- FUNCTION: May be involved in H(2) production during fermentative growth
CC       (PubMed:24025676). Involved in the export and assembly of a fimbrial
CC       subunit across the outer membrane (Probable).
CC       {ECO:0000269|PubMed:24025676, ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Loss of H(2) production on minimal glucose and
CC       complex glucose but not on complex formate medium. Alters production of
CC       organic acids when grown on minimal glucose medium. This phenotype was
CC       complemented by reintroduction of the ygiG gene.
CC       {ECO:0000269|PubMed:24025676}.
CC   -!- SIMILARITY: Belongs to the fimbrial export usher family. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. It is missing about 10
CC       N-terminal amino acids compared to orthologs. {ECO:0000305}.
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DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP009048; BAA16575.2; -; Genomic_DNA.
DR   PIR; D65092; D65092.
DR   RefSeq; WP_000016375.1; NZ_LN832404.1.
DR   AlphaFoldDB; P76655; -.
DR   SMR; P76655; -.
DR   BioGRID; 4262383; 229.
DR   DIP; DIP-12877N; -.
DR   STRING; 316407.85675849; -.
DR   PRIDE; P76655; -.
DR   EnsemblBacteria; BAA16575; BAA16575; BAA16575.
DR   KEGG; ecj:JW5507; -.
DR   PATRIC; fig|83333.103.peg.3947; -.
DR   EchoBASE; EB3979; -.
DR   eggNOG; COG3188; Bacteria.
DR   HOGENOM; CLU_009120_1_1_6; -.
DR   InParanoid; P76655; -.
DR   OMA; RANYAKR; -.
DR   PhylomeDB; P76655; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015473; F:fimbrial usher porin activity; IBA:GO_Central.
DR   GO; GO:0009297; P:pilus assembly; IBA:GO_Central.
DR   Gene3D; 2.60.40.2070; -; 1.
DR   Gene3D; 2.60.40.2610; -; 1.
DR   Gene3D; 3.10.20.410; -; 1.
DR   InterPro; IPR000015; Fimb_usher.
DR   InterPro; IPR018030; Fimbrial_membr_usher_CS.
DR   InterPro; IPR042186; FimD_plug_dom.
DR   InterPro; IPR025949; PapC-like_C.
DR   InterPro; IPR043142; PapC-like_C_sf.
DR   InterPro; IPR025885; PapC_N.
DR   InterPro; IPR037224; PapC_N_sf.
DR   PANTHER; PTHR30451; PTHR30451; 1.
DR   Pfam; PF13953; PapC_C; 1.
DR   Pfam; PF13954; PapC_N; 1.
DR   Pfam; PF00577; Usher; 1.
DR   SUPFAM; SSF141729; SSF141729; 1.
DR   PROSITE; PS01151; FIMBRIAL_USHER; 1.
PE   5: Uncertain;
KW   Cell outer membrane; Disulfide bond; Fimbrium biogenesis; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane beta strand;
KW   Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..821
FT                   /note="Putative outer membrane usher protein YqiG"
FT                   /id="PRO_0000009334"
FT   DISULFID        798..817
FT                   /evidence="ECO:0000255"
FT   CONFLICT        728..730
FT                   /note="AIG -> CYW (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   821 AA;  91792 MW;  1FED7878909583BE CRC64;
     MSGNIGANPV IIIGCASAYA VEFNKDLIEA EDRENVNLSQ FETDGQLPVG KYSLSTLINN
     KRTPIHLDLQ WVLIDNQTAV CVTPEQLTLL GFTDEFIEKT QQNLIDGCYP IEKEKQITTY
     LDKGKMQLSI SAPQAWLKYK DANWTPPELW NHGIAGAFLD YNLYASHYAP HQGDNSQNIS
     SYGQAGVNLG AWRLRTDYQY DQSFNNGKSQ ATNLDFPRIY LFRPIPAMNA KLTIGQYDTE
     SSIFDSFHFS GISLKSDENM LPPDLRGYAP QITGVAQTNA KVTVSQNNRI IYQENVPPGP
     FAITNLFNTL QGQLDVKVEE EDGRVTQWQV ASNSIPYLTR KGQIRYTTAM GKPTSVGGDS
     LQQPFFWTGE FSWGWLNNVS LYGGSVLTNR DYQSLAAGVG FNLNSLGSLS FDVTRSDAQL
     HNQDKETGYS YRANYSKRFE STGSQLTFAG YRFSDKNFVT MNEYINDTNH YTNYQNEKES
     YIVTFNQYLE SLRLNTYVSL ARNTYWDASS NVNYSLSLSR DFDIGPLKNV STSLTFSRIN
     WEEDNQDQLY LNISIPWGTS RTLSYGMQRN QDNEISHTAS WYDSSDRNNS WSVSASGDND
     EFKDMKASLR ASYQHNTENG RLYLSGTSQR DSYYSLNASW NGSFTATRHG AAFHDYSGSA
     DSRFMIDADG TEDIPLNNKR AVTNRYGIGV IPSVSSYITT SLSVDTRNLP ENVDIENSVI
     TTTLTEGAIG YAKLDTRKGY QIIGVIRLAD GSHPPLGISV KDETSHKELG LVADGGFVYL
     NGIQDDNKLA LRWGDKSCFI QPPNSSNLTT GTAILPCISQ N