YQIK_ECOLI
ID YQIK_ECOLI Reviewed; 553 AA.
AC P77306; Q6J1J6;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Flotillin family inner membrane protein YqiK;
GN Name=yqiK; OrderedLocusNames=b3051, JW3023;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 529-553.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Perna N.T.;
RT "Escherichia coli K-12 MG1655 genomic sequence correction.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=DL95;
RX PubMed=20713508; DOI=10.1101/gad.1945010;
RA Lopez D., Kolter R.;
RT "Functional microdomains in bacterial membranes.";
RL Genes Dev. 24:1893-1902(2010).
RN [7]
RP DISRUPTION PHENOTYPE.
RC STRAIN=K12 / BW25113;
RX PubMed=31062059; DOI=10.1007/s00203-019-01670-8;
RA Padilla-Vaca F., Vargas-Maya N.I., Elizarraras-Vargas N.U.,
RA Rangel-Serrano A., Cardoso-Reyes L.R., Razo-Soria T.,
RA Membrillo-Hernandez J., Franco B.;
RT "Flotillin homologue is involved in the swimming behavior of Escherichia
RT coli.";
RL Arch. Microbiol. 201:999-1008(2019).
CC -!- FUNCTION: Found in membrane microdomains that may be equivalent to
CC eukaryotic membrane rafts (PubMed:20713508). FMMs are highly dynamic
CC and increase in number as cells age. Flotillins are thought to be
CC important factors in membrane fluidity (By similarity).
CC {ECO:0000250|UniProtKB:O32076, ECO:0000269|PubMed:20713508}.
CC -!- SUBUNIT: Homooligomerizes. {ECO:0000250|UniProtKB:O32076}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996,
CC ECO:0000269|PubMed:20713508}; Single-pass membrane protein
CC {ECO:0000255}. Membrane raft {ECO:0000269|PubMed:20713508}; Single-pass
CC membrane protein {ECO:0000255}. Note=Present in detergent-resistant
CC membrane (DRM) fractions, approximately 6 foci per cell.
CC {ECO:0000269|PubMed:20713508}.
CC -!- DISRUPTION PHENOTYPE: No visible effect on resistance to oxidizing
CC agents, proton motive force-damaging agents, membrane or cell wall
CC stress. Improved resistance to ampicillin, enhanced swimming ability.
CC {ECO:0000269|PubMed:31062059}.
CC -!- SIMILARITY: Belongs to the band 7/mec-2 family. Flotillin subfamily.
CC {ECO:0000305}.
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DR EMBL; U00096; AAC76087.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16578.1; -; Genomic_DNA.
DR EMBL; AY605712; AAT28328.1; -; Genomic_DNA.
DR PIR; A65093; A65093.
DR RefSeq; NP_417523.1; NC_000913.3.
DR RefSeq; WP_001311137.1; NZ_SSZK01000028.1.
DR AlphaFoldDB; P77306; -.
DR SMR; P77306; -.
DR BioGRID; 4259252; 14.
DR IntAct; P77306; 4.
DR STRING; 511145.b3051; -.
DR PaxDb; P77306; -.
DR PRIDE; P77306; -.
DR EnsemblBacteria; AAC76087; AAC76087; b3051.
DR EnsemblBacteria; BAA16578; BAA16578; BAA16578.
DR GeneID; 947537; -.
DR KEGG; ecj:JW3023; -.
DR KEGG; eco:b3051; -.
DR PATRIC; fig|1411691.4.peg.3680; -.
DR EchoBASE; EB3983; -.
DR eggNOG; COG2268; Bacteria.
DR HOGENOM; CLU_013048_1_0_6; -.
DR InParanoid; P77306; -.
DR OMA; ERQYDSE; -.
DR PhylomeDB; P77306; -.
DR BioCyc; EcoCyc:G7589-MON; -.
DR PRO; PR:P77306; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR031905; Flotillin_C.
DR InterPro; IPR027705; Flotillin_fam.
DR PANTHER; PTHR13806; PTHR13806; 1.
DR Pfam; PF01145; Band_7; 1.
DR Pfam; PF15975; Flot; 1.
DR SMART; SM00244; PHB; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..553
FT /note="Flotillin family inner membrane protein YqiK"
FT /id="PRO_0000169419"
FT TOPO_DOM 1..9
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..553
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
SQ SEQUENCE 553 AA; 60700 MW; A8B63EE29854AD74 CRC64;
MDDIVNSVPS WMFTAIIAVC ILFIIGIIFA RLYRRASAEQ AFVRTGLGGQ KVVMSGGAIV
MPIFHEIIPI NMNTLKLEVS RSTIDSLITK DRMRVDVVVA FFVRVKPSVE GIATAAQTLG
QRTLSPEDLR MLVEDKFVDA LRATAAQMTM HELQDTRENF VQGVQNTVAE DLSKNGLELE
SVSLTNFNQT SKEHFNPNNA FDAEGLTKLT QETERRRRER NEVEQDVEVA VREKNRDALS
RKLEIEQQEA FMTLEQEQQV KTRTAEQNAR IAAFEAERRR EAEQTRILAE RQIQETEIDR
EQAVRSRKVE AEREVRIKEI EQQQVTEIAN QTKSIAIAAK SEQQSQAEAR ANLALAEAVS
AQQNVETTRQ TAEADRAKQV ALIAAAQDAE TKAVELTVRA KAEKEAAEMQ AAAIVELAEA
TRKKGLAEAE AQRALNDAIN VLSDEQTSLK FKLALLQALP AVIEKSVEPM KSIDGIKIIQ
VDGLNRGGAA GDANTGNVGG GNLAEQALSA ALSYRTQAPL IDSLLNEIGV SGGSLAALTS
PLTSTTPVEE KAE
