ID   YR355_MIMIV             Reviewed;         302 AA.
AC   Q5UQV0;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Putative thiol protease R355;
DE            EC=3.4.22.-;
GN   OrderedLocusNames=MIMI_R355;
OS   Acanthamoeba polyphaga mimivirus (APMV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Imitervirales; Mimiviridae; Mimivirus.
OX   NCBI_TaxID=212035;
OH   NCBI_TaxID=5757; Acanthamoeba polyphaga (Amoeba).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Rowbotham-Bradford;
RX   PubMed=15486256; DOI=10.1126/science.1101485;
RA   Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H.,
RA   La Scola B., Susan M., Claverie J.-M.;
RT   "The 1.2-megabase genome sequence of Mimivirus.";
RL   Science 306:1344-1350(2004).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=16971431; DOI=10.1128/jvi.00940-06;
RA   Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H.,
RA   Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.;
RT   "Mimivirus giant particles incorporate a large fraction of anonymous and
RT   unique gene products.";
RL   J. Virol. 80:11678-11685(2006).
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:16971431}.
CC   -!- SIMILARITY: Belongs to the peptidase C48 family. {ECO:0000305}.
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DR   EMBL; AY653733; AAV50624.1; -; Genomic_DNA.
DR   RefSeq; YP_003986858.1; NC_014649.1.
DR   SMR; Q5UQV0; -.
DR   GeneID; 9924974; -.
DR   KEGG; vg:9924974; -.
DR   Proteomes; UP000001134; Genome.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0019082; P:viral protein processing; IEA:InterPro.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR003653; Peptidase_C48_C.
DR   InterPro; IPR016510; VPRT.
DR   Pfam; PF02902; Peptidase_C48; 1.
DR   PIRSF; PIRSF007159; Peptidase_ASVF; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS50600; ULP_PROTEASE; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Protease; Reference proteome; Thiol protease; Virion.
FT   CHAIN           1..302
FT                   /note="Putative thiol protease R355"
FT                   /id="PRO_0000244782"
FT   ACT_SITE        182
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        199
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        244
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   302 AA;  34896 MW;  08658E0BD78B44D1 CRC64;
     MNICGPQRYD KENNTCFNVD QLVEMAKAYN RYLSKTKLNP SRNYHFGDAD LINIKSDKKY
     LLKQFKDRFG KICGSDEICL THQAFMGELV GEMKDDILFG TFRSEGPSKS TEWLSTIDIN
     QIMVPYENIY PNFKFIGAVP ADCDQVSVCP LYNINYDKLM DEGINYIATI FNHDRYGQPG
     SHWVAMFVDI NNGKLYYCDS NGKEPTKYIE NSIEKFAQFY KRKTGNDIIY KYNKNSYQKD
     GSECGVYSCN FIIRMLSGEP FDNIVSNSLS FQEINSCRNV YFRNQPSKFK PHKLCDPTNS
     GK