ID   YR563_MIMIV             Reviewed;         573 AA.
AC   Q7T6X3;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 2.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Putative ATP-dependent RNA helicase R563;
DE            EC=3.6.4.13;
GN   OrderedLocusNames=MIMI_R563;
OS   Acanthamoeba polyphaga mimivirus (APMV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Imitervirales; Mimiviridae; Mimivirus.
OX   NCBI_TaxID=212035;
OH   NCBI_TaxID=5757; Acanthamoeba polyphaga (Amoeba).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Rowbotham-Bradford;
RX   PubMed=15486256; DOI=10.1126/science.1101485;
RA   Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H.,
RA   La Scola B., Susan M., Claverie J.-M.;
RT   "The 1.2-megabase genome sequence of Mimivirus.";
RL   Science 306:1344-1350(2004).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=16971431; DOI=10.1128/jvi.00940-06;
RA   Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H.,
RA   Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.;
RT   "Mimivirus giant particles incorporate a large fraction of anonymous and
RT   unique gene products.";
RL   J. Virol. 80:11678-11685(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:16971431}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AY653733; AAQ09587.2; -; Genomic_DNA.
DR   RefSeq; YP_003987078.1; NC_014649.1.
DR   SMR; Q7T6X3; -.
DR   PRIDE; Q7T6X3; -.
DR   GeneID; 9925199; -.
DR   KEGG; vg:9925199; -.
DR   Proteomes; UP000001134; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW   Virion.
FT   CHAIN           1..573
FT                   /note="Putative ATP-dependent RNA helicase R563"
FT                   /id="PRO_0000244775"
FT   DOMAIN          57..233
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          374..551
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           179..182
FT                   /note="DEAH box"
FT   BINDING         70..77
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   573 AA;  66973 MW;  3FD7AF2FF32A06DD CRC64;
     MSSRLSYVNV DDPNFYEFID EKYSKYKIPP KQKTFKQFCF PSKYEFQIPQ QFLAEYINPK
     TPYKGLLIYH RIGAGKTCTA IKIAENFKNK SKIMIVVPAS LKGNFRSELR SLCADDHYLT
     SKERSELKIL HPSSDEYKSI IKVSDDRIDK YYTIYSYNKF VDLIKQNKIN LTNTLLIIDE
     VHNMISETGT YYESLYETIH SAPDNMRLVI MTATPIFDKP NEIALTMNLL VRNKQLPVGP
     DFVSTFMDIR YNSKGPVYHV KNMDLFKEFV KGYVSYYRGA PPYVFPKSEL FFVRTKMSDL
     QKTVYQKITG KEVKQTKVRD YVNENISNNF FIGTRMISNI VYPNEKVGLK GYNSLTDEDL
     TIAKIREYSP KFLKILRKIK RCNGTVFVYS NFKEYGGIRV FARLLEFHRF KNYEFNGSGP
     RRFAIWSGDQ DPIYKEEVKA VFNNKDNEFG SKIKVILGSS SIKEGVSFLR VQEVHIMEPY
     WNFSRMEQII GRAIRFCSHK DVELDRQLVK VYIYLAVHPD IKMSIDERMM KMALDKKMIN
     SAFEKALKEA AIDCELFKNA NVYPGEQDIQ CEQ