ID   YRF18_YEAST             Reviewed;        1796 AA.
AC   P0CX22; D6VTG2; O94087; P24088; P24089; Q53WX0; Q99313;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Y' element ATP-dependent helicase protein 1 copy 8;
DE            EC=3.6.4.12;
GN   Name=YRF1-8; OrderedLocusNames=YOR396W; ORFNames=O7535;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 90839 / S288c / YP1;
RX   PubMed=1628806; DOI=10.1093/genetics/131.3.559;
RA   Louis E.J., Haber J.E.;
RT   "The structure and evolution of subtelomeric Y' repeats in Saccharomyces
RT   cerevisiae.";
RL   Genetics 131:559-574(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 90839 / S288c / YP1;
RX   PubMed=7785338; DOI=10.1002/yea.320110410;
RA   Pryde F.E., Huckle T.C., Louis E.J.;
RT   "Sequence analysis of the right end of chromosome XV in Saccharomyces
RT   cerevisiae: an insight into the structural and functional significance of
RT   sub-telomeric repeat sequences.";
RL   Yeast 11:371-382(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA   Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA   Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA   Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA   Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA   Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA   Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA   Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA   Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA   Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA   Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA   Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA   Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=9837911; DOI=10.1074/jbc.273.50.33360;
RA   Yamada M., Hayatsu N., Matsuura A., Ishikawa F.;
RT   "Y'-Help1, a DNA helicase encoded by the yeast subtelomeric Y' element, is
RT   induced in survivors defective for telomerase.";
RL   J. Biol. Chem. 273:33360-33366(1998).
CC   -!- FUNCTION: Catalyzes DNA unwinding and is involved in telomerase-
CC       independent telomere maintenance. {ECO:0000269|PubMed:9837911}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- INDUCTION: Induced in absence of telomerase TLC1.
CC       {ECO:0000269|PubMed:9837911}.
CC   -!- SIMILARITY: Belongs to the helicase family. Yeast subtelomeric Y'
CC       repeat subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC48994.1; Type=Frameshift; Note=Produces 2 separate ORFs.; Evidence={ECO:0000305};
CC       Sequence=AAC48995.1; Type=Frameshift; Note=Produces 2 separate ORFs.; Evidence={ECO:0000305};
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DR   EMBL; U23472; AAC48994.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; U23472; AAC48995.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; Z75302; CAA99727.1; -; Genomic_DNA.
DR   EMBL; BK006948; DAA11154.1; -; Genomic_DNA.
DR   PIR; S65004; S65004.
DR   RefSeq; NP_010834.1; NM_001180853.1.
DR   RefSeq; NP_013572.3; NM_001182355.3.
DR   RefSeq; NP_061495.4; NM_001184447.3.
DR   AlphaFoldDB; P0CX22; -.
DR   BioGRID; 31723; 19.
DR   BioGRID; 32590; 30.
DR   BioGRID; 34775; 4.
DR   iPTMnet; P0CX22; -.
DR   PRIDE; P0CX22; -.
DR   EnsemblFungi; YDR545W_mRNA; YDR545W; YDR545W.
DR   EnsemblFungi; YLR467W_mRNA; YLR467W; YLR467W.
DR   EnsemblFungi; YOR396W_mRNA; YOR396W; YOR396W.
DR   GeneID; 851189; -.
DR   GeneID; 852158; -.
DR   GeneID; 854577; -.
DR   KEGG; sce:YDR545W; -.
DR   KEGG; sce:YLR467W; -.
DR   KEGG; sce:YOR396W; -.
DR   SGD; S000007526; YRF1-8.
DR   VEuPathDB; FungiDB:YDR545W; -.
DR   VEuPathDB; FungiDB:YLR467W; -.
DR   VEuPathDB; FungiDB:YOR396W; -.
DR   GeneTree; ENSGT00940000153173; -.
DR   HOGENOM; CLU_003044_2_0_1; -.
DR   BioCyc; YEAST:G3O-33895-MON; -.
DR   PRO; PR:P0CX22; -.
DR   Proteomes; UP000002311; Chromosome XV.
DR   RNAct; P0CX22; protein.
DR   ExpressionAtlas; P0CX22; baseline.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004386; F:helicase activity; ISS:SGD.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0000722; P:telomere maintenance via recombination; IEA:UniProt.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR021646; Sir1_ORC-binding.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF11603; Sir1; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW   Repeat.
FT   CHAIN           1..1796
FT                   /note="Y' element ATP-dependent helicase protein 1 copy 8"
FT                   /id="PRO_0000409757"
FT   DOMAIN          797..974
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          1031..1180
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          743..767
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1254..1278
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1294..1421
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        745..767
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1294..1397
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1398..1421
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         810..817
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   CONFLICT        1293
FT                   /note="I -> T (in Ref. 1; AAC48995)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1796 AA;  203781 MW;  BC241E78ABBB6FF1 CRC64;
     MKVSDRRKFE KANFDEFESA LNNKNDLVHC PSITLFESIP TEVRSFYEDE KSGLIKVVKF
     RTGAMDRKRS FEKIVISVMV GKNVQKFLTF VEDEPDFQGG PIPSKYLIPK KINLMVYTLF
     QVHTLKFNRK DYDTLSLFYL NRGYYNELSF RVLERCHEIA SARPNDSSTM RTFTDFVSGA
     PIVRSLQKST IRKYGYNLAP YMFLLLHVDE LSIFSAYQAS LPGEKKVDTE RLKRDLCPRK
     PIEIKYFSQI CNDMMNKKDR LGDILHIILR ACALNFGAGP RGGAGDEEDR SITNEEPIIP
     SVDEHGLKVC KLRSPNTPRR LRKTLDAVKA LLVSSCACTA RDLDIFDDTN GVAMWKWIKI
     LYHEVAQETT LKDSYRITLV PSSDGISVCG KLFNREYVRG FYFACKAQFD NLWGELNNCF
     YMPTVVDIAS LILRNREVLF REPKRGIDEY LENDSFLQMI PVKYREIVLP KLRRDTNKMT
     AALKNKVTVA IDELTVPLMW MVHFAVGYPY RYPELQLLAF AGPQRNVYVD DTTRRIQLYT
     DYNKNGSSEP RLKTLDGLTS DYVFYFVTVL RQMQICALGN SYDAFNHDPW MDVVGFEDPD
     QVTNRDISRI VLYSYMFLNT AKGCLVEYAT FRQYMRELPK NAPQKLNFRE MRQGLIALGR
     HCVGSRFETD LYESATSELM ANHSVQTGRN IYGVDSFSLT SVSGTTATLL QERASERWIQ
     WLGLESDYHC SFSSTRNAED VVAGEAASSD HDQKISRVTR KRPREPKSTN DILVAGQKLF
     GSSFEFRDLH QLRLCHEIYM ADTPSVAVQA PPGYGKTELF HLPLIALASK GDVKYVSFLF
     VPYTVLLANC MIRLSRCGCL NVAPVRNFIE EGCDGVTDLY VGIYDDLAST NFTDRIAAWE
     NIVECTFRTN NVKLGYLIVD EFHNFETEVY RQSQFGGITN LDFDAFEKAI FLSGTAPEAV
     ADAALQRIGL TGLAKKSMDI NELKRSEDLS RGLSSYPTRM FNLIKEKSEV PLGHVHKIWK
     KVESQPEEAL KLLLALFEIE PESKAIVVAS TTNEVEELAC SWRKYFRVVW IHGKLGAAEK
     VSRTKEFVTD GSMRVLIGTK LVTEGIDIKQ LMMVIMLDNR LNIIELIQGV GRLRDGGLCY
     LLSRKNSWAA RNRKGELPPI KEGCITEQVR EFYGLESKKG KKGQHVGCCG SRTDLSADTV
     ELIERMDRLA EKQATASMSI IALPSSFQES NSSDRCRKYC SSDEDSDTCI HGSANASTNA
     TTNSSTNATT TASTNVRTSA TTTASINVRT SAITTESTNS STNATTTAST NVRTSATTTA
     SINVRTSATT TESTNSNTSA TTTESTDSNT SATTTESTDS NTSATTTAST NSSTNATTTA
     STNSSTNATT TESTNASAKE DANKDGNAED NRFHPVTDIN KESYKRKGSQ MVLLERKKLK
     AQFPNTSENM NVLQFLGFRS DEIKHLFLYG IDVYFCPEGV FTQYGLCKGC QKMFELCVCW
     AGQKVSYRRM AWEALAVERM LRNDEEYKEY LEDIEPYHGD PVGYLKYFSV KRGEIYSQIQ
     RNYAWYLAIT RRRETISVLD STRGKQGSQV FRMSGRQIKE LYYKVWSNLR ESKTEVLQYF
     LNWDEKKCRE EWEAKDDTVF VEALEKVGVF QRLRSMTSAG LQGPQYVKLQ FSRHHRQLRS
     RYELSLGMHL RDQLALGVTP SKVPHWTAFL SMLIGLFYNK TFRQKLEYLL EQISEVWLLP
     HWLDLANVEV LAADNTRVPL YMLMVAVHKE LDSDDVPDGR FDIILLCRDS SREVGE