CBS1_YEAST
ID CBS1_YEAST Reviewed; 229 AA.
AC P14066; D6VRS9;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Cytochrome b translational activator protein CBS1, mitochondrial;
DE AltName: Full=COB-specific translational activator protein CBS1;
DE Flags: Precursor;
GN Name=CBS1; OrderedLocusNames=YDL069C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2671648; DOI=10.1007/bf00330956;
RA Koerte A., Forsbach V., Gottenoef T., Roedel G.;
RT "In vitro and in vivo studies on the mitochondrial import of CBS1, a
RT translational activator of cytochrome b in yeast.";
RL Mol. Gen. Genet. 217:162-167(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
RX PubMed=2550765; DOI=10.1007/bf00330565;
RA Forsbach V., Pillar T., Gottenoef T., Roedel G.;
RT "Chromosomal localization and expression of CBS1, a translational activator
RT of cytochrome b in yeast.";
RL Mol. Gen. Genet. 218:57-63(1989).
RN [5]
RP PROTEIN SEQUENCE OF 26-29, AND PROTEOLYTIC PROCESSING.
RX PubMed=1657414; DOI=10.1007/bf00312770;
RA Koerte A., Michaelis U., Lottspeich F., Roedel G.;
RT "Over-expression, purification and determination of the proteolytic
RT processing site of the yeast mitochondrial CBS1 protein.";
RL Curr. Genet. 20:87-90(1991).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=1745228; DOI=10.1007/bf00290666;
RA Michaelis U., Korte A., Rodel G.;
RT "Association of cytochrome b translational activator proteins with the
RT mitochondrial membrane: implications for cytochrome b expression in
RT yeast.";
RL Mol. Gen. Genet. 230:177-185(1991).
RN [7]
RP MUTAGENESIS OF LYS-98; GLY-100; ILE-124 AND PHE-129.
RX PubMed=10669873;
RX DOI=10.1002/1097-0061(20000315)16:4<353::aid-yea539>3.0.co;2-#;
RA Krause-Buchholz U., Tzschoppe K., Paret C., Ostermann K., Roedel G.;
RT "Identification of functionally important regions of the Saccharomyces
RT cerevisiae mitochondrial translational activator Cbs1p.";
RL Yeast 16:353-363(2000).
RN [8]
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
CC -!- FUNCTION: mRNA-specific translational activator of cytochrome b. The
CC cytochrome b (COB) leader RNA may represent the target sequence for
CC CBS1 and CBS2, tethering the COB mRNA to the inner mitochondrial
CC membrane, where cotranslational insertion of cytochrome b into the
CC membrane can occur.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:14576278, ECO:0000269|PubMed:1745228}; Peripheral
CC membrane protein {ECO:0000269|PubMed:14576278,
CC ECO:0000269|PubMed:1745228}.
CC -!- MISCELLANEOUS: Present with 556 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- MISCELLANEOUS: CBS1 protein devoid of mitochondrial targeting sequences
CC can enter mitochondria in vivo, possibly due to a bypass of the
CC mitochondrial import system.
CC -!- MISCELLANEOUS: The in vitro imported CBS1 protein is functional and
CC soluble indicating that the N-terminal part of CBS1 confers some
CC binding to mitochondrial membranes.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA33676.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA34251.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA98633.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X15650; CAA33676.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z74117; CAA98633.1; ALT_INIT; Genomic_DNA.
DR EMBL; X16120; CAA34251.1; ALT_INIT; Genomic_DNA.
DR EMBL; BK006938; DAA11789.1; -; Genomic_DNA.
DR PIR; JQ0296; JQ0296.
DR RefSeq; NP_010214.2; NM_001180128.1.
DR AlphaFoldDB; P14066; -.
DR BioGRID; 31991; 124.
DR IntAct; P14066; 3.
DR MINT; P14066; -.
DR STRING; 4932.YDL069C; -.
DR MaxQB; P14066; -.
DR PaxDb; P14066; -.
DR PRIDE; P14066; -.
DR EnsemblFungi; YDL069C_mRNA; YDL069C; YDL069C.
DR GeneID; 851491; -.
DR KEGG; sce:YDL069C; -.
DR SGD; S000002227; CBS1.
DR VEuPathDB; FungiDB:YDL069C; -.
DR HOGENOM; CLU_105542_0_0_1; -.
DR InParanoid; P14066; -.
DR BioCyc; YEAST:G3O-29481-MON; -.
DR PRO; PR:P14066; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P14066; protein.
DR GO; GO:0031314; C:extrinsic component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005761; C:mitochondrial ribosome; IPI:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0045182; F:translation regulator activity; IMP:SGD.
DR GO; GO:0034551; P:mitochondrial respiratory chain complex III assembly; IMP:SGD.
DR GO; GO:0070131; P:positive regulation of mitochondrial translation; IMP:SGD.
PE 1: Evidence at protein level;
KW Activator; Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transit peptide;
KW Translation regulation.
FT TRANSIT 1..25
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:1657414"
FT CHAIN 26..229
FT /note="Cytochrome b translational activator protein CBS1,
FT mitochondrial"
FT /id="PRO_0000020850"
FT MUTAGEN 98
FT /note="K->R: Loss of function, causes respiratory
FT deficiency."
FT /evidence="ECO:0000269|PubMed:10669873"
FT MUTAGEN 100
FT /note="G->A: In CBS1-1, which is respiratory deficient."
FT /evidence="ECO:0000269|PubMed:10669873"
FT MUTAGEN 124
FT /note="I->T: Loss of function, causes respiratory
FT deficiency."
FT /evidence="ECO:0000269|PubMed:10669873"
FT MUTAGEN 129
FT /note="F->S,T: Loss of function, causes respiratory
FT deficiency."
FT /evidence="ECO:0000269|PubMed:10669873"
SQ SEQUENCE 229 AA; 26586 MW; 7EEFF36FE15F3692 CRC64;
MLRTKVFATT VARISGIRRY IPIRTINTVT KKNISKIEKL CEVLEVNPDG YKGKERIPTK
ELTKLLYTTS RNMLVRVPMT GDLSTGNTFE TRNETLQKLG EQLIHLEINK MLTITFTNFN
QFNIMNKNFN YIHNLDRARV VNMDSISWLI KNSLKINQLA HLRIPANLPK EMGLTSSSND
FQNLNDWKVI LSFIGYLKLL EIKNDNKKFI ESIIKTICIP LINYHLRKS
