ID   CBS2_YEAST              Reviewed;         389 AA.
AC   P14905; D6VSI0;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 160.
DE   RecName: Full=Cytochrome B translational activator protein CBS2;
GN   Name=CBS2; Synonyms=CBP7; OrderedLocusNames=YDR197W; ORFNames=YD9346.08;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3070350; DOI=10.1007/bf00337720;
RA   Michaelis U., Schlapp T., Roedel G.;
RT   "Yeast nuclear gene CBS2, required for translational activation of
RT   cytochrome b, encodes a basic protein of 45 kDa.";
RL   Mol. Gen. Genet. 214:263-270(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   SUBCELLULAR LOCATION, AND LACK OF PROCESSING.
RX   PubMed=2270079; DOI=10.1007/bf00264445;
RA   Michaelis U., Roedel G.;
RT   "Identification of CBS2 as a mitochondrial protein in Saccharomyces
RT   cerevisiae.";
RL   Mol. Gen. Genet. 223:394-400(1990).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Translational activator of cytochrome b. The cytochrome b
CC       (coB) leader RNA may represent the target sequence for CBS1 and/ or
CC       CBS2.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:2270079}.
CC   -!- MISCELLANEOUS: Present with 2140 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; X13523; CAA31876.1; -; Genomic_DNA.
DR   EMBL; Z48784; CAA88710.1; -; Genomic_DNA.
DR   EMBL; AY558577; AAS56903.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA12040.1; -; Genomic_DNA.
DR   PIR; S22839; S22839.
DR   RefSeq; NP_010483.1; NM_001180505.1.
DR   AlphaFoldDB; P14905; -.
DR   SMR; P14905; -.
DR   BioGRID; 32249; 146.
DR   IntAct; P14905; 4.
DR   STRING; 4932.YDR197W; -.
DR   CarbonylDB; P14905; -.
DR   MaxQB; P14905; -.
DR   PaxDb; P14905; -.
DR   PRIDE; P14905; -.
DR   EnsemblFungi; YDR197W_mRNA; YDR197W; YDR197W.
DR   GeneID; 851778; -.
DR   KEGG; sce:YDR197W; -.
DR   SGD; S000002605; CBS2.
DR   VEuPathDB; FungiDB:YDR197W; -.
DR   eggNOG; ENOG502RI8G; Eukaryota.
DR   HOGENOM; CLU_031468_10_2_1; -.
DR   InParanoid; P14905; -.
DR   OMA; NCKWNDI; -.
DR   BioCyc; YEAST:G3O-29783-MON; -.
DR   PRO; PR:P14905; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; P14905; protein.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0031314; C:extrinsic component of mitochondrial inner membrane; IDA:SGD.
DR   GO; GO:0005761; C:mitochondrial ribosome; IPI:SGD.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0008677; F:2-dehydropantoate 2-reductase activity; IBA:GO_Central.
DR   GO; GO:0050661; F:NADP binding; IBA:GO_Central.
DR   GO; GO:0045182; F:translation regulator activity; IMP:SGD.
DR   GO; GO:0070131; P:positive regulation of mitochondrial translation; IMP:SGD.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR013752; KPA_reductase.
DR   Pfam; PF08546; ApbA_C; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
PE   1: Evidence at protein level;
KW   Activator; Mitochondrion; Reference proteome; Translation regulation.
FT   CHAIN           1..389
FT                   /note="Cytochrome B translational activator protein CBS2"
FT                   /id="PRO_0000089382"
SQ   SEQUENCE   389 AA;  44608 MW;  45866D1EF6737FB3 CRC64;
     MSSSIPRVYS LGNSAMTYLL ALRIAQLPSQ PKVPSVVLLL NDQKKLNRFL NNDSKIIVKS
     SNNNKETYHR QFMASCVPPI LSNGEIAPIE NLIVSDPSSK FITAQLSKYN KSLRPETNIL
     FLNPSLNLLE HLHRYRWRFD EARPNLFMGF TTPVDVGTIH QEFQLSLKVK GRIQFHIAKI
     DGFPRMSSTG KSASLSLRGD RQKNEKENNA FYKLFREISR LRSGIGSDLV SFDLHVHGFQ
     DLFFTELEKL ILESCTEPLL AVYDCVYKKE LLKIPGAQDI IKKLISEQLS IIDRSYPSLN
     TNPNYSVIFD KERIFSLVMR DLEVNGHKRA KLAQSLNQLN QTNINELNGF FVSLGKYKKC
     NCKWNDILLT LIKGKQFITK QKALDYHYL