ID   YRVJ_BACSU              Reviewed;         518 AA.
AC   O32041;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Putative N-acetylmuramoyl-L-alanine amidase YrvJ;
DE            EC=3.5.1.28;
DE   Flags: Precursor;
GN   Name=yrvJ; OrderedLocusNames=BSU27580;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: Probably involved in cell-wall metabolism. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L-
CC         amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28;
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
CC       {ECO:0000305}.
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DR   EMBL; AL009126; CAB14717.1; -; Genomic_DNA.
DR   PIR; B69981; B69981.
DR   RefSeq; NP_390636.1; NC_000964.3.
DR   RefSeq; WP_004399040.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; O32041; -.
DR   SMR; O32041; -.
DR   STRING; 224308.BSU27580; -.
DR   PaxDb; O32041; -.
DR   PRIDE; O32041; -.
DR   DNASU; 937544; -.
DR   EnsemblBacteria; CAB14717; CAB14717; BSU_27580.
DR   GeneID; 937544; -.
DR   KEGG; bsu:BSU27580; -.
DR   PATRIC; fig|224308.179.peg.2997; -.
DR   eggNOG; COG0860; Bacteria.
DR   eggNOG; COG3103; Bacteria.
DR   eggNOG; COG4991; Bacteria.
DR   InParanoid; O32041; -.
DR   OMA; SIHYDSY; -.
DR   PhylomeDB; O32041; -.
DR   BioCyc; BSUB:BSU27580-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR   CDD; cd02696; MurNAc-LAA; 1.
DR   InterPro; IPR002508; MurNAc-LAA_cat.
DR   InterPro; IPR017293; N-acetylmuramoyl-L-ala_amidase.
DR   InterPro; IPR003646; SH3-like_bac-type.
DR   Pfam; PF01520; Amidase_3; 1.
DR   Pfam; PF08239; SH3_3; 4.
DR   PIRSF; PIRSF037846; Autolysin_YrvJ_prd; 1.
DR   SMART; SM00646; Ami_3; 1.
DR   SMART; SM00287; SH3b; 4.
DR   PROSITE; PS51781; SH3B; 4.
PE   3: Inferred from homology;
KW   Cell wall; Cell wall biogenesis/degradation; Hydrolase; Metal-binding;
KW   Reference proteome; Secreted; Signal; Zinc.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..518
FT                   /note="Putative N-acetylmuramoyl-L-alanine amidase YrvJ"
FT                   /id="PRO_0000360805"
FT   DOMAIN          29..91
FT                   /note="SH3b 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01117"
FT   DOMAIN          102..164
FT                   /note="SH3b 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01117"
FT   DOMAIN          181..243
FT                   /note="SH3b 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01117"
FT   DOMAIN          258..320
FT                   /note="SH3b 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01117"
FT   DOMAIN          346..514
FT                   /note="MurNAc-LAA"
FT                   /evidence="ECO:0000255"
FT   REGION          94..121
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          160..186
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          322..352
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        94..114
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   518 AA;  55501 MW;  8FE642A3D7724135 CRC64;
     MNKKYFVLIV CIIFTSALFP TFSSVTAAQG EAVIATDEMN VRSGPGLSYG ITAEVKKGER
     YPILKEDGDW VQIQLGSGEK GWVVSWLITK EDQASTSSSG SSDTVTSTDP DLRMRSGPGT
     SYEVIGKFPQ GSQASVIDKD SGWIKISYHS ATGWVSSEYV TSGGSSSASD ESDQTEDSGA
     STTGTVGVSS LNVRASASHD AAIITKLDRG TKLTVLNEKN GWAHIEVNGL KGWVASHYLL
     TSSVPADDSA NAGSSSSAKK AYIMYGGTNL RSDASTSASI VERAAKGDSY TITGSKGSWY
     EIKLDNGQTA YVANWVVQTS KSAEEAGEPP VSDSPSGNGS LNNKTIIVDP GHGGKDSGTI
     GYSGKFEKNL TIKTAKLLAS KLRSAGADVY VTRQDDTFVS LQSRVSTSHY RNADAFISIH
     YDSYADTSTR GSTAYYYSPA KDQELASDVH SEVVKRSSIP DRGVLFGDYY VLRENRQPAM
     LYELGYVSHP QEEAIVHSNS YQEKVTDGIE SGLEKYFQ