YSL12_ORYSJ
ID YSL12_ORYSJ Reviewed; 717 AA.
AC Q5JQD7; Q0JBM2; Q25CH4;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Probable metal-nicotianamine transporter YSL12;
DE AltName: Full=Protein YELLOW STRIPE LIKE 12;
DE Short=OsYSL12;
GN Name=YSL12; OrderedLocusNames=Os04g0524600, LOC_Os04g44320;
GN ORFNames=OSJNBb0065J09.17;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Nipponbare;
RX PubMed=15255870; DOI=10.1111/j.1365-313x.2004.02146.x;
RA Koike S., Inoue H., Mizuno D., Takahashi M., Nakanishi H., Mori S.,
RA Nishizawa N.K.;
RT "OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron
RT and expressed in the phloem.";
RL Plant J. 39:415-424(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=19049971; DOI=10.1074/jbc.m806042200;
RA Inoue H., Kobayashi T., Nozoye T., Takahashi M., Kakei Y., Suzuki K.,
RA Nakazono M., Nakanishi H., Mori S., Nishizawa N.K.;
RT "Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid Transporter
RT expressed in the roots and is essential for iron uptake in early growth of
RT the seedlings.";
RL J. Biol. Chem. 284:3470-3479(2009).
CC -!- FUNCTION: May be involved in the transport of nicotianamine-chelated
CC metals. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5JQD7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5JQD7-2; Sequence=VSP_036329;
CC -!- TISSUE SPECIFICITY: Expressed in root cortex and stele.
CC {ECO:0000269|PubMed:19049971}.
CC -!- SIMILARITY: Belongs to the YSL (TC 2.A.67.2) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE91892.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAI44637.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB190922; BAE91892.1; ALT_FRAME; mRNA.
DR EMBL; AL663010; CAI44637.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008210; BAF15265.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS90151.1; -; Genomic_DNA.
DR EMBL; AK069437; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015635779.1; XM_015780293.1. [Q5JQD7-1]
DR AlphaFoldDB; Q5JQD7; -.
DR STRING; 4530.OS04T0524600-01; -.
DR PaxDb; Q5JQD7; -.
DR PRIDE; Q5JQD7; -.
DR EnsemblPlants; Os04t0524600-01; Os04t0524600-01; Os04g0524600. [Q5JQD7-2]
DR GeneID; 4336442; -.
DR Gramene; Os04t0524600-01; Os04t0524600-01; Os04g0524600. [Q5JQD7-2]
DR KEGG; osa:4336442; -.
DR eggNOG; ENOG502QQ2H; Eukaryota.
DR InParanoid; Q5JQD7; -.
DR OMA; FGMSDIV; -.
DR OrthoDB; 525938at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR Genevisible; Q5JQD7; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0035673; F:oligopeptide transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR004813; OPT.
DR InterPro; IPR045035; YSL-like.
DR PANTHER; PTHR31645; PTHR31645; 1.
DR Pfam; PF03169; OPT; 1.
DR TIGRFAMs; TIGR00728; OPT_sfam; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..717
FT /note="Probable metal-nicotianamine transporter YSL12"
FT /id="PRO_0000363875"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 536..556
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 593..613
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 636..656
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 671..691
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..32
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 8..148
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_036329"
FT CONFLICT 251
FT /note="F -> L (in Ref. 6; AK069437)"
FT /evidence="ECO:0000305"
FT CONFLICT 361
FT /note="D -> N (in Ref. 1; BAE91892)"
FT /evidence="ECO:0000305"
FT CONFLICT 373
FT /note="T -> K (in Ref. 1; BAE91892)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 717 AA; 77692 MW; EFBEEE02E13EC627 CRC64;
MASHANASGG GGDEEMVEAS TLRHRHGAGK DANGVGTERQ LAAAAAEGEE EGPSSVERAF
VDRAVPSWRE QLTVRAFVVS FFLSIMFSII VMKLNLTTGI IPSLNVSAGL LGFFFVRLWT
AAIERVGLLR QPFTRQENTV IQTCVVAAYG IAFSGGFGTY LFGMSETIAK QATEANNAQN
VKNPHIGWMI GFLFLVSFIG LLALVPLRKI MIVDYKLTYP SGTATAYLIN GFHTPEGAKL
AKKQVKELGK FFLFSFVWGF FQWFYTAGDG CGFQSFPTLG LQAYKNRFYF DFSPTYVGVG
MICPHIVNVS VLLGGILSWG IMWPLIRNKK GSWYAASLSE TSLHGLQGYR VFISIALILG
DGLYNFVKVL IRTTAGFVVM MKKNSTLPVS NNGSPMVATE AISFDDERRT ELFLKDQIPK
TVAFGGYVAV AAVSIGTLPQ IFPQLKWYYI LVAYVFAPVL AFCNAYGAGL TDWSLASTYG
KLAIFIFGAW AGASNGGVLV GLAACGVMMS IVSTASDLMQ DFKTGYLTLA SPRSMFVSQV
IGTAMGCVIA PCVFWLFYKA FADIGVSGTE YPAPYAIVYR NMAILGVDGF SSLPKHCLTL
CYIFFAAAIA INLARDLAPS KVARFIPLPM AMAIPFYIGS YFAIDMFIGT VILFVWEMVN
KAKAEAFAPA VASGLICGDG IWTLPQSILA LAKVKPPICM KFLSRSVNAQ VDGFLGN
