YSL2_ARATH
ID YSL2_ARATH Reviewed; 664 AA.
AC Q6R3K9; Q0WUE8; Q8L750; Q9FIN4;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Metal-nicotianamine transporter YSL2;
DE AltName: Full=Protein YELLOW STRIPE LIKE 2;
DE Short=AtYSL2;
GN Name=YSL2; OrderedLocusNames=At5g24380; ORFNames=K16H17.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Roberts L.A., Walker E.L.;
RT "The yellow stripe-like (YSL) family of metal-nicotianamine transporters.";
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=15753101; DOI=10.1093/pcp/pci081;
RA Schaaf G., Schikora A., Haeberle J., Vert G., Ludewig U., Briat J.-F.,
RA Curie C., von Wiren N.;
RT "A putative function for the arabidopsis Fe-Phytosiderophore transporter
RT homolog AtYSL2 in Fe and Zn homeostasis.";
RL Plant Cell Physiol. 46:762-774(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 174-664.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 217-664.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=15308754; DOI=10.1105/tpc.104.023150;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Phosphoproteomics of the Arabidopsis plasma membrane and a new
RT phosphorylation site database.";
RL Plant Cell 16:2394-2405(2004).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=15255869; DOI=10.1111/j.1365-313x.2004.02128.x;
RA DiDonato R.J. Jr., Roberts L.A., Sanderson T., Eisley R.B., Walker E.L.;
RT "Arabidopsis Yellow Stripe-Like2 (YSL2): a metal-regulated gene encoding a
RT plasma membrane transporter of nicotianamine-metal complexes.";
RL Plant J. 39:403-414(2004).
CC -!- FUNCTION: May be involved in the lateral transport of nicotianamine-
CC chelated metals in the vasculature. {ECO:0000269|PubMed:15255869}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15255869};
CC Multi-pass membrane protein {ECO:0000269|PubMed:15255869}. Note=Found
CC almost exclusively on the lateral plasma membranes.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves and weakly in shoots.
CC Restricted to the veins, to the central cylinder of the young roots and
CC to the pericycle and the endodermis cells facing the meta-xylem tubes
CC in older roots. Expressed in the vasculature of sepals, petals,
CC anthers, stigma and siliques, but not in developing seeds or in
CC meristematic zones. {ECO:0000269|PubMed:15255869,
CC ECO:0000269|PubMed:15753101}.
CC -!- INDUCTION: Inhibition upon iron and zinc deficiency. Not regulated by
CC copper. Subjected to diurnal regulation. {ECO:0000269|PubMed:15753101}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:15255869}.
CC -!- SIMILARITY: Belongs to the YSL (TC 2.A.67.2) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM98073.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB11231.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY515561; AAS00692.1; -; mRNA.
DR EMBL; AY648977; AAT69741.1; -; mRNA.
DR EMBL; AB016884; BAB11231.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED93305.1; -; Genomic_DNA.
DR EMBL; AK227212; BAE99250.1; -; mRNA.
DR EMBL; AY139751; AAM98073.1; ALT_INIT; mRNA.
DR EMBL; BT005806; AAO64741.1; -; mRNA.
DR RefSeq; NP_197826.2; NM_122346.3.
DR AlphaFoldDB; Q6R3K9; -.
DR STRING; 3702.AT5G24380.1; -.
DR TCDB; 2.A.67.2.3; the oligopeptide transporter (opt) family.
DR iPTMnet; Q6R3K9; -.
DR PaxDb; Q6R3K9; -.
DR PRIDE; Q6R3K9; -.
DR ProteomicsDB; 242920; -.
DR EnsemblPlants; AT5G24380.1; AT5G24380.1; AT5G24380.
DR GeneID; 832509; -.
DR Gramene; AT5G24380.1; AT5G24380.1; AT5G24380.
DR KEGG; ath:AT5G24380; -.
DR Araport; AT5G24380; -.
DR TAIR; locus:2152896; AT5G24380.
DR eggNOG; ENOG502QQ2H; Eukaryota.
DR HOGENOM; CLU_015477_2_0_1; -.
DR InParanoid; Q6R3K9; -.
DR OMA; MNFTAAH; -.
DR OrthoDB; 525938at2759; -.
DR PhylomeDB; Q6R3K9; -.
DR PRO; PR:Q6R3K9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6R3K9; baseline and differential.
DR Genevisible; Q6R3K9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0051980; F:iron-nicotianamine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0035673; F:oligopeptide transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0010039; P:response to iron ion; IEP:TAIR.
DR GO; GO:0010043; P:response to zinc ion; IEP:TAIR.
DR GO; GO:0048316; P:seed development; IBA:GO_Central.
DR InterPro; IPR004813; OPT.
DR InterPro; IPR045035; YSL-like.
DR PANTHER; PTHR31645; PTHR31645; 1.
DR Pfam; PF03169; OPT; 1.
DR TIGRFAMs; TIGR00728; OPT_sfam; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..664
FT /note="Metal-nicotianamine transporter YSL2"
FT /id="PRO_0000311413"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..516
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 549..569
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 594..614
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 629..649
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 664 AA; 72983 MW; 2EC4CA84F9A75A13 CRC64;
MENERVEREQ SQFQEDEFID SRKPPPWRKQ ITVRAIVASL LIGIVYSVIC LKLNLTTGLV
PNLNISSALL AFVFLKSWTK VLQKAGIATT PFTRQENTIA QTCAVACYSI SLAGGFASYL
LGLNRRTYEE TGVNTEGNNP RGIKEPGVGW MTSFLFVTSF IGLVVLVPLR KVMIIDYKLT
YPSGTATAVL INGFHTSKGD KTAKKQIRGF IKSFGLSFFW AFFGWFYSGG EKCGFSQFPT
FGLQALDKTF YFDFSMTYVG AGMICSHLVN LSLLFGAILS WGIMWPLIAR LKGEWFPATL
KDNSMQGLNG YKVFICIALI LGDGLYNFVK ILFFTGRSFH SRLSKTNSIS TLVEVPEDST
KESDNLKREN EVFVRESIPL WMACVGYLFF SLVSIIAIPL MFPQLKWYFV LVAYLLAPSL
SFCNAYGAGL TDMNMAYNYG KAALFVMAAL AGKNDGVVAG MVACGLIKSI VSVSADLMHD
FKTGHLTQTS PRSMLVAQAI GTAIGCVVAP LTFFLFYKAF DVGNQNGEYK APYAMIYRNM
AIIGVQGPSA LPKHCLELCY GFFAFAVAAN LARDLLPDKP GKWIPLPMAM AVPFLVGGSF
AIDMCIGSLV VYVWKKVNRK KADVMVPAVA SGLICGDGLW ILPSSLLALA KVRPPICMNF
TAAH