YSL2_ORYSJ
ID YSL2_ORYSJ Reviewed; 674 AA.
AC Q6H3Z6; A0A0P0VMG1; Q5TM89;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Metal-nicotianamine transporter YSL2;
DE AltName: Full=Protein YELLOW STRIPE LIKE 2;
DE Short=OsYSL2;
GN Name=YSL2; OrderedLocusNames=Os02g0649900, LOC_Os02g43370;
GN ORFNames=OSJNBb0012J10.2, P0048B08.26;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Nipponbare;
RX PubMed=15255870; DOI=10.1111/j.1365-313x.2004.02146.x;
RA Koike S., Inoue H., Mizuno D., Takahashi M., Nakanishi H., Mori S.,
RA Nishizawa N.K.;
RT "OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron
RT and expressed in the phloem.";
RL Plant J. 39:415-424(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Involved in the phloem transport of iron and manganese and
CC their translocation into the grain. Transports iron- and manganese-
CC nicotianamine chelates, but not iron-phytosiderophore.
CC {ECO:0000269|PubMed:15255870}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:15255870};
CC Multi-pass membrane protein {ECO:0000305|PubMed:15255870}.
CC -!- TISSUE SPECIFICITY: Expressed in phloem cells of vascular bundles in
CC leaves and leaf sheaths. Expressed at low levels in phloem companion
CC cells in the central cylinder of roots, but not in the epidermal or
CC cortical cells. {ECO:0000269|PubMed:15255870}.
CC -!- DEVELOPMENTAL STAGE: Before anthesis, expressed in the vascular bundles
CC of spikelets and at low levels in the central region of anthers. After
CC fertilization, expression in bundles increases especially in the upper
CC part of the husk. At 5 days after anthesis, highly expressed in the
CC developing ovary and 3 days later in the developing embryo and outer
CC layer of the endosperm. From 20 to 30 days after anthesis, expressed in
CC mature embryo and peripheral layer of the endosperm.
CC {ECO:0000269|PubMed:15255870}.
CC -!- INDUCTION: In leaves by iron deficiency. {ECO:0000269|PubMed:15255870}.
CC -!- SIMILARITY: Belongs to the YSL (TC 2.A.67.2) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD25583.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD26553.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB126253; BAD72770.1; -; mRNA.
DR EMBL; AB164646; BAD90812.1; -; mRNA.
DR EMBL; AP004868; BAD25583.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP007203; BAD26553.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008208; BAF09500.2; -; Genomic_DNA.
DR EMBL; AP014958; BAS80047.1; -; Genomic_DNA.
DR RefSeq; XP_015625412.1; XM_015769926.1.
DR AlphaFoldDB; Q6H3Z6; -.
DR STRING; 4530.OS02T0649900-00; -.
DR TCDB; 2.A.67.2.11; the oligopeptide transporter (opt) family.
DR PaxDb; Q6H3Z6; -.
DR PRIDE; Q6H3Z6; -.
DR EnsemblPlants; Os02t0649900-01; Os02t0649900-01; Os02g0649900.
DR GeneID; 4330161; -.
DR Gramene; Os02t0649900-01; Os02t0649900-01; Os02g0649900.
DR KEGG; osa:4330161; -.
DR eggNOG; ENOG502QQ2H; Eukaryota.
DR HOGENOM; CLU_015477_2_1_1; -.
DR InParanoid; Q6H3Z6; -.
DR OMA; ANFAVDM; -.
DR OrthoDB; 525938at2759; -.
DR BioCyc; MetaCyc:MON-14021; -.
DR PlantReactome; R-OSA-9025727; Iron uptake and transport in root vascular system.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6H3Z6; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0051980; F:iron-nicotianamine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0035673; F:oligopeptide transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0010039; P:response to iron ion; IBA:GO_Central.
DR GO; GO:0048316; P:seed development; IBA:GO_Central.
DR InterPro; IPR004813; OPT.
DR InterPro; IPR045035; YSL-like.
DR PANTHER; PTHR31645; PTHR31645; 1.
DR Pfam; PF03169; OPT; 1.
DR TIGRFAMs; TIGR00728; OPT_sfam; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..674
FT /note="Metal-nicotianamine transporter YSL2"
FT /id="PRO_0000363865"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 420..440
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..526
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 559..579
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 604..624
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 633..653
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 103
FT /note="R -> W (in Ref. 1; BAD90812/BAD72770)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 674 AA; 73343 MW; 81F8D005792F7865 CRC64;
MEAAAPEIER CDAGDVESDH DGAAAAAERV PPWREQVTAR GMVAALLIGF VYTVIIMKLA
LTTGIIPTLN VSAALLAFLA LRGWTRAPAL LLPGGGAASS SSRRRPFTRQ ENTVVQTCAV
ACYTMGFGGG FGSSLLALNR KTYELAGVST PGNSPGSYKE PGVGWMTGFL FAISFVGLLN
LLPLRKALII DYKLTYPSGT ATAVLINGFH TPQGENSAKK QVRGFLNCFG ISLLWSFFQW
FYTGGESCGF LQFPTFGLKA WKQTFYFDFS LTYVGAGMIC SHLVNLSALF GAILSWGIMW
PLISIQKGKW YPGNVPESSM TSLFGYKSFM CVALIMGDGL YHFIKVTGIT AKSLHERSNR
RHAKKATDED TFVIADMQRD EFFNKDYIPN WLAYAGYALL SIVAVIAIPI MFQQVKWYYV
VVAFVLAPVL GFSNAYGTGL TDMNMSYNYG KIALFIFAAW GGRDNGVIAG LVGCGIVKQL
VQVSADLMHD FKTGHLTLTS PRSMLVGQAI GTAMGCIIAP LTFLLFYKAF DIGNPDGYWK
APYALIFRNM AILGVEGFSA LPKHCLELSA GFFAFSVLIN LMRDFLPRKY RDYVPLPTAM
AVPFLVGANF AIDMCVGSLI VFAWHKINSK ESALLVPAVA SGFICGDGIW MFPSSLLSLA
KVKPPICMKF TPGS
