YSL8_ARATH
ID YSL8_ARATH Reviewed; 724 AA.
AC Q6R3K4; Q94BX5; Q9SX70;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Probable metal-nicotianamine transporter YSL8;
DE AltName: Full=Protein YELLOW STRIPE LIKE 8;
DE Short=AtYSL8;
GN Name=YSL8; OrderedLocusNames=At1g48370; ORFNames=F11A17.8, F11A17_27;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Roberts L.A., Walker E.L.;
RT "The yellow stripe-like (YSL) family of metal-nicotianamine transporters.";
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17869214; DOI=10.1016/j.bbrc.2007.08.177;
RA Hem S., Rofidal V., Sommerer N., Rossignol M.;
RT "Novel subsets of the Arabidopsis plasmalemma phosphoproteome identify
RT phosphorylation sites in secondary active transporters.";
RL Biochem. Biophys. Res. Commun. 363:375-380(2007).
CC -!- FUNCTION: May be involved in the transport of nicotianamine-chelated
CC metals. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the YSL (TC 2.A.67.2) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD49762.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY515566; AAS00697.1; -; mRNA.
DR EMBL; AC007932; AAD49762.2; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32281.1; -; Genomic_DNA.
DR EMBL; AY039600; AAK62655.1; -; mRNA.
DR EMBL; BT010472; AAQ65095.1; -; mRNA.
DR PIR; G96523; G96523.
DR RefSeq; NP_564525.1; NM_103733.4.
DR AlphaFoldDB; Q6R3K4; -.
DR BioGRID; 26482; 1.
DR IntAct; Q6R3K4; 1.
DR STRING; 3702.AT1G48370.1; -.
DR iPTMnet; Q6R3K4; -.
DR PaxDb; Q6R3K4; -.
DR PRIDE; Q6R3K4; -.
DR ProteomicsDB; 242972; -.
DR EnsemblPlants; AT1G48370.1; AT1G48370.1; AT1G48370.
DR GeneID; 841257; -.
DR Gramene; AT1G48370.1; AT1G48370.1; AT1G48370.
DR KEGG; ath:AT1G48370; -.
DR Araport; AT1G48370; -.
DR TAIR; locus:2007715; AT1G48370.
DR eggNOG; ENOG502QUDW; Eukaryota.
DR HOGENOM; CLU_015477_2_0_1; -.
DR InParanoid; Q6R3K4; -.
DR OMA; IQTCVIA; -.
DR OrthoDB; 525938at2759; -.
DR PhylomeDB; Q6R3K4; -.
DR PRO; PR:Q6R3K4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6R3K4; baseline and differential.
DR Genevisible; Q6R3K4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0035673; F:oligopeptide transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR004813; OPT.
DR InterPro; IPR045035; YSL-like.
DR PANTHER; PTHR31645; PTHR31645; 1.
DR Pfam; PF03169; OPT; 1.
DR TIGRFAMs; TIGR00728; OPT_sfam; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..724
FT /note="Probable metal-nicotianamine transporter YSL8"
FT /id="PRO_0000311419"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..497
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 501..520
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 541..561
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 603..623
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 641..661
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 679..699
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 386..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..28
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 25
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17869214"
FT CONFLICT 90
FT /note="I -> V (in Ref. 1; AAS00697)"
FT /evidence="ECO:0000305"
FT CONFLICT 117
FT /note="K -> E (in Ref. 1; AAS00697)"
FT /evidence="ECO:0000305"
FT CONFLICT 494
FT /note="A -> V (in Ref. 1; AAS00697)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 724 AA; 80109 MW; 087FE20B9E79D940 CRC64;
MRKGGLTPDR DRQIEEHELQ ETGISPDIER LKRNINATPY QREEEEEDRE EQEESVEGIF
ESREVPSWKK QLTIRAFVVS FALSILFSFI VMKLNLTTGI IPSLNVSAGL LGFFFVKTWT
KMLHKSGLLK QPFTRQENTV IQTCVVASSG IAFSGGFGTY LFAMSHRIAD QSGDVARGVK
DPSLGWMIAF LFVVSFLGLF SVVPLRKIMI IDFKLPYPSG TATAHLINSF HTPQGAKLAK
KQVRVLGKFF SFSFFWGFFQ WFFTAGENCG FNSFPTFGLR AYQYKFYFDF SATYVGVGMI
CPYIINISLL LGGILSWGLM WPLIETRKGD WFPSNVDSSS MNGLQAYKVF IAVATILGDG
LYNFCKVLIR TFSGLISQIR GKAGSRSSLA HKEDPPASPA SPLTPRISYD DQRRTRFFLK
DQIPSWFAVG GYVVISAVST AILPHMFSQL RWYYIIVIYI FAPILAFCNA YGAGLTDWSL
ASTYGKLAIF TIGAWAGSDH GGLLAGLAAC GVMMNIVSTA SDLTQDFKTG YLTLSSPRAM
FVSQVIGTAM GCLVSPCVFW LFYKAFDDLG LPNSEYPAPF ATVYRSMAKL GVEGVSSLPR
DCLMLCYVFF GVAILINLIK DCLGNRWGRF VPLPMAMAIP FFLGPYFAID MCVGSFILFV
WERLDAPKAE AFATAVASGL ICGDGIWTLP SSVLAIAGVK PPICMKFLSA ATNHRVDKFL
QGSS
