CBT_ORYSJ
ID CBT_ORYSJ Reviewed; 927 AA.
AC Q7XHR2; Q2WEL1;
DT 22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Calmodulin-binding transcription activator CBT;
DE AltName: Full=CaM-binding transcription factor {ECO:0000303|PubMed:16192280};
DE Short=OsCBT {ECO:0000303|PubMed:16192280};
GN Name=CBT {ECO:0000303|PubMed:16192280};
GN OrderedLocusNames=Os07g0490200 {ECO:0000312|EMBL:BAF21586.1},
GN LOC_Os07g30774 {ECO:0000305};
GN ORFNames=P0477A12.32 {ECO:0000312|EMBL:BAC80067.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ACTIVITY REGULATION, SUBCELLULAR
RP LOCATION, CALMODULIN-BINDING, AND MUTAGENESIS OF ILE-764; VAL-829 AND
RP TRP-836.
RX PubMed=16192280; DOI=10.1074/jbc.m504616200;
RA Choi M.S., Kim M.C., Yoo J.H., Moon B.C., Koo S.C., Park B.O., Lee J.H.,
RA Koo Y.D., Han H.J., Lee S.Y., Chung W.S., Lim C.O., Cho M.J.;
RT "Isolation of a calmodulin-binding transcription factor from rice (Oryza
RT sativa L.).";
RL J. Biol. Chem. 280:40820-40831(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Transcription activator that binds calmodulin in a calcium-
CC dependent manner in vitro. Binds to the DNA consensus sequence 5'-
CC T[AC]CG[CT]GT[GT][GT][GT][GT]T[GT]CG-3'. {ECO:0000269|PubMed:16192280}.
CC -!- ACTIVITY REGULATION: Transcriptional activation activity is strongly
CC reduced by calmodulin. {ECO:0000269|PubMed:16192280}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00767,
CC ECO:0000269|PubMed:16192280}.
CC -!- SIMILARITY: Belongs to the CAMTA family. {ECO:0000305}.
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DR EMBL; AF499741; AAQ07306.1; -; mRNA.
DR EMBL; AP005190; BAC80067.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21586.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT01546.1; -; Genomic_DNA.
DR RefSeq; XP_015646432.1; XM_015790946.1.
DR AlphaFoldDB; Q7XHR2; -.
DR SMR; Q7XHR2; -.
DR STRING; 4530.OS07T0490200-01; -.
DR PaxDb; Q7XHR2; -.
DR PRIDE; Q7XHR2; -.
DR EnsemblPlants; Os07t0490200-01; Os07t0490200-01; Os07g0490200.
DR GeneID; 4343265; -.
DR Gramene; Os07t0490200-01; Os07t0490200-01; Os07g0490200.
DR eggNOG; KOG0520; Eukaryota.
DR HOGENOM; CLU_005708_1_0_1; -.
DR InParanoid; Q7XHR2; -.
DR OMA; RRNFINM; -.
DR OrthoDB; 156204at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR ExpressionAtlas; Q7XHR2; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005516; F:calmodulin binding; IDA:UniProtKB.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR005559; CG-1_dom.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF03859; CG-1; 1.
DR Pfam; PF00612; IQ; 3.
DR SMART; SM00248; ANK; 2.
DR SMART; SM01076; CG-1; 1.
DR SMART; SM00015; IQ; 3.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS51437; CG_1; 1.
DR PROSITE; PS50096; IQ; 4.
PE 1: Evidence at protein level;
KW Activator; ANK repeat; Calcium; Calmodulin-binding; DNA-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..927
FT /note="Calmodulin-binding transcription activator CBT"
FT /id="PRO_0000442291"
FT REPEAT 609..638
FT /note="ANK"
FT /evidence="ECO:0000255"
FT DOMAIN 757..786
FT /note="IQ 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 799..828
FT /note="IQ 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 882..911
FT /note="IQ 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DNA_BIND 26..152
FT /note="CG-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00767"
FT REGION 70..96
FT /note="Necessary and sufficient for nuclear localization"
FT /evidence="ECO:0000269|PubMed:16192280"
FT REGION 826..845
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000269|PubMed:16192280"
FT REGION 830..851
FT /note="Necessary and sufficient for nuclear localization"
FT /evidence="ECO:0000269|PubMed:16192280"
FT MOTIF 72..79
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00767"
FT MUTAGEN 764
FT /note="I->E: Abolishes calmodulin binding."
FT /evidence="ECO:0000269|PubMed:16192280"
FT MUTAGEN 829
FT /note="V->R: Abolishes calmodulin binding; when associated
FT with R-836."
FT /evidence="ECO:0000269|PubMed:16192280"
FT MUTAGEN 836
FT /note="W->R: Abolishes calmodulin binding; when associated
FT with R-829."
FT /evidence="ECO:0000269|PubMed:16192280"
FT CONFLICT 27
FT /note="E -> D (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
FT CONFLICT 119
FT /note="D -> N (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
FT CONFLICT 153..154
FT /note="AP -> VL (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
FT CONFLICT 174..175
FT /note="SP -> FL (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
FT CONFLICT 340
FT /note="S -> I (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
FT CONFLICT 345
FT /note="K -> N (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
FT CONFLICT 388
FT /note="E -> D (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
FT CONFLICT 403
FT /note="K -> N (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
FT CONFLICT 762
FT /note="M -> L (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
FT CONFLICT 842
FT /note="G -> A (in Ref. 1; AAQ07306)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 927 AA; 103614 MW; EA42C05066B71F58 CRC64;
MAGAGGWDPL VGSEIHGFLT YPDLNYEKLV AEAAARWFRP NEIYAILANH ARFKIHAQPV
DKPVSGTVVL YDRKVVRNFR KDGHNWKKKK DGRTVQEAHE KLKIGNEERV HVYYARGEDD
PNFFRRCYWL LDKDLERIVL VHYRQTAEEN AMAPPNPEPE VADVPTVNLI HYTSPLTSAD
STSGHTELSL PEEINSHGGI SASSETGNHD SSLEEFWANL LESSIKNDPK VVTSACGGSF
VSSQQINNGP KNSGNIVNTS MASNAIPALN VVSETYATNH GLNQVNANHF GALKHQGDQT
QSLLASDVDS QSDQFISSSV KSPMDGNTSI PNEVPARQNS LGLWKYLDDD SPGLGDNPSS
VPQSFCPVTN ERLLEINEIS PEWAYSTETT KVVVIGNFYE QYKHLAGSAM FGVFGEQCVA
GDIVQTGVYR FMVGPHTPGK VDFYLTLDGK TPISEICSFT YHVMHGSSLE ARLPPSEDDY
KRTNLKMQMR LARLLFATNK KKIAPKLLVE GTKVANLMSA LPEKEWMDLW NILSDPEGTY
VPVTESLLEL VLRNRLQEWL VEMVMEGHKS TGRDDLGQGA IHLCSFLGYT WAIRLFSLSG
FSLDFRDSSG WTALHWAAYH GRERMVATLL SAGANPSLVT DPTPESPAGL TAADLAARQG
YDGLAAYLAE KGLTAHFEAM SLSKDTEQSP SKTRLTKLQS EKFEHLSEQE LCLKESLAAY
RNAADAASNI QAALRERTLK LQTKAIQLAN PEIEASEIVA AMKIQHAFRN YNRKKAMRAA
ARIQSHFRTW KMRRNFINMR RQVIRIQAAY RGHQVRRQYR KVIWSVGIVE KAILRWRKKR
KGLRGIASGM PVVMTVDAEA EPASTAEEDF FQAGRQQAED RFNRSVVRVQ ALFRSYKAQQ
EYRRMKIAHE EAKIEFSEGQ LGAACRS
