CBWD1_MOUSE
ID CBWD1_MOUSE Reviewed; 393 AA.
AC Q8VEH6; Q7TSA8; Q8K399;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=COBW domain-containing protein 1;
DE AltName: Full=Cobalamin synthase W domain-containing protein 1;
GN Name=Cbwd1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Extraembryonic tissue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and Czech II; TISSUE=Limb, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VEH6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VEH6-2; Sequence=VSP_019735;
CC -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. CobW
CC subfamily. {ECO:0000305}.
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DR EMBL; AK132291; BAE21084.1; -; mRNA.
DR EMBL; BC018472; AAH18472.1; -; mRNA.
DR EMBL; BC027643; AAH27643.1; -; mRNA.
DR EMBL; BC053745; AAH53745.1; -; mRNA.
DR CCDS; CCDS37942.1; -. [Q8VEH6-1]
DR RefSeq; NP_666209.1; NM_146097.3. [Q8VEH6-1]
DR AlphaFoldDB; Q8VEH6; -.
DR SMR; Q8VEH6; -.
DR BioGRID; 230464; 3.
DR STRING; 10090.ENSMUSP00000025815; -.
DR PhosphoSitePlus; Q8VEH6; -.
DR REPRODUCTION-2DPAGE; Q8VEH6; -.
DR EPD; Q8VEH6; -.
DR MaxQB; Q8VEH6; -.
DR PaxDb; Q8VEH6; -.
DR PeptideAtlas; Q8VEH6; -.
DR PRIDE; Q8VEH6; -.
DR ProteomicsDB; 281413; -. [Q8VEH6-1]
DR ProteomicsDB; 281414; -. [Q8VEH6-2]
DR DNASU; 226043; -.
DR Ensembl; ENSMUST00000025815; ENSMUSP00000025815; ENSMUSG00000024878. [Q8VEH6-1]
DR GeneID; 226043; -.
DR KEGG; mmu:226043; -.
DR UCSC; uc008haw.2; mouse. [Q8VEH6-1]
DR CTD; 55871; -.
DR MGI; MGI:2385089; Cbwd1.
DR VEuPathDB; HostDB:ENSMUSG00000024878; -.
DR eggNOG; KOG2743; Eukaryota.
DR GeneTree; ENSGT00640000091523; -.
DR HOGENOM; CLU_017452_0_1_1; -.
DR InParanoid; Q8VEH6; -.
DR OMA; MWGVLRY; -.
DR OrthoDB; 1144889at2759; -.
DR PhylomeDB; Q8VEH6; -.
DR TreeFam; TF332679; -.
DR BioGRID-ORCS; 226043; 8 hits in 75 CRISPR screens.
DR ChiTaRS; Cbwd1; mouse.
DR PRO; PR:Q8VEH6; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q8VEH6; protein.
DR Bgee; ENSMUSG00000024878; Expressed in spermatocyte and 225 other tissues.
DR ExpressionAtlas; Q8VEH6; baseline and differential.
DR Genevisible; Q8VEH6; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1220.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011629; Cbl_biosynth_CobW-like_C.
DR InterPro; IPR036627; CobW-likC_sf.
DR InterPro; IPR003495; CobW/HypB/UreG_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF02492; cobW; 1.
DR Pfam; PF07683; CobW_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Nucleotide-binding; Reference proteome.
FT CHAIN 1..393
FT /note="COBW domain-containing protein 1"
FT /id="PRO_0000245524"
FT DOMAIN 271..374
FT /note="CobW C-terminal"
FT BINDING 47..54
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 108..112
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 201..204
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..121
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019735"
SQ SEQUENCE 393 AA; 43772 MW; 0BBBC3F0B6EDFCDF CRC64;
MLPAVKTVEA EEEYAEDCPE LVPIETKNQE EENLDFITKI PVTIVTGYLG AGKTTLLNYI
LTEQHNRKIA VILNEFGEGS AVEKSLAVSQ GGELYEEWLE LRNGCLCCSV KDSGLRAIEN
LMQKKGKFDY ILLETTGLAD PGAVASMFWV DAELGSDIYL DGIITVVDSK YGLKHLTEEK
PDGLVNEATR QVALADMILI NKTDLVSEEE LNNLRTTIRS INGLGKVLET QRSRVHLSNI
LDLHAYDILS GISLQKKLQH VSTAPHLDQS IVTVTFEVPG SAKEECLNVF IQNLLWEKNV
KNKDGHCMEV IRLKGLVSIK DKPQQMIVQG IHELYDLEES LVNWKDDAER ACQLVFIGRN
LDKDVLQQLF LTAVAEAEEQ RTAPGRDGVC PSH
