ID   YSY6_YEAST              Reviewed;          65 AA.
AC   P38374; D6VQF8;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Protein YSY6;
GN   Name=YSY6; OrderedLocusNames=YBR162W-A; ORFNames=YBR162BW;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=TD-1;
RX   PubMed=1938999; DOI=10.1093/oxfordjournals.jbchem.a123460;
RA   Sakaguchi M., Ueguchi C., Ito K., Omura T.;
RT   "Yeast gene which suppresses the defect in protein export of a secY mutant
RT   of E. coli.";
RL   J. Biochem. 109:799-802(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=18724936; DOI=10.1016/j.cell.2008.06.025;
RA   Schuldiner M., Metz J., Schmid V., Denic V., Rakwalska M., Schmitt H.D.,
RA   Schwappach B., Weissman J.S.;
RT   "The GET complex mediates insertion of tail-anchored proteins into the ER
RT   membrane.";
RL   Cell 134:634-645(2008).
CC   -!- FUNCTION: May interact with target proteins during translocation into
CC       the lumen of the endoplasmic reticulum. May protect unfolded target
CC       proteins against degradation and facilitate correct glycosylation
CC       (Potential). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC       Single-pass type IV membrane protein {ECO:0000305}; Cytoplasmic side
CC       {ECO:0000305}.
CC   -!- MISCELLANEOUS: Present with 846 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the RAMP4 family. {ECO:0000305}.
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DR   EMBL; D00919; BAA00764.1; -; Genomic_DNA.
DR   EMBL; Z36031; CAA85121.1; -; Genomic_DNA.
DR   EMBL; Z36032; CAA85124.1; -; Genomic_DNA.
DR   EMBL; AY558124; AAS56450.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07278.1; -; Genomic_DNA.
DR   PIR; JQ0912; JQ0912.
DR   RefSeq; NP_009721.1; NM_001180059.1.
DR   AlphaFoldDB; P38374; -.
DR   SMR; P38374; -.
DR   BioGRID; 32862; 93.
DR   DIP; DIP-4936N; -.
DR   IntAct; P38374; 5.
DR   STRING; 4932.YBR162W-A; -.
DR   PaxDb; P38374; -.
DR   PRIDE; P38374; -.
DR   EnsemblFungi; YBR162W-A_mRNA; YBR162W-A; YBR162W-A.
DR   GeneID; 852460; -.
DR   KEGG; sce:YBR162W-A; -.
DR   SGD; S000002158; YSY6.
DR   VEuPathDB; FungiDB:YBR162W-A; -.
DR   eggNOG; ENOG502SDGZ; Eukaryota.
DR   HOGENOM; CLU_182424_0_1_1; -.
DR   InParanoid; P38374; -.
DR   OMA; AIQTPKQ; -.
DR   BioCyc; YEAST:G3O-29230-MON; -.
DR   Reactome; R-SCE-9609523; Insertion of tail-anchored proteins into the endoplasmic reticulum membrane.
DR   PRO; PR:P38374; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P38374; protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; IBA:GO_Central.
DR   GO; GO:0006486; P:protein glycosylation; IBA:GO_Central.
DR   GO; GO:0009306; P:protein secretion; IGI:SGD.
DR   InterPro; IPR010580; ER_stress-assoc.
DR   Pfam; PF06624; RAMP4; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Membrane; Protein transport; Reference proteome;
KW   Translocation; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..65
FT                   /note="Protein YSY6"
FT                   /id="PRO_0000066513"
FT   TOPO_DOM        1..44
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   65 AA;  7365 MW;  326410AB4772B33D CRC64;
     MAVQTPRQRL ANAKFNKNNE KYRKYGKKKE GKTEKTAPVI SKTWLGILLF LLVGGGVLQL
     ISYIL