ID   YTBE_BACSU              Reviewed;         280 AA.
AC   O34678; Q795V7;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Uncharacterized oxidoreductase YtbE;
DE            EC=1.-.-.-;
GN   Name=ytbE; OrderedLocusNames=BSU29050;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT   200 kb rrnB-dnaB region.";
RL   Microbiology 143:3431-3441(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR   EMBL; AF008220; AAC00406.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14865.1; -; Genomic_DNA.
DR   PIR; D69988; D69988.
DR   RefSeq; NP_390783.1; NC_000964.3.
DR   RefSeq; WP_003229455.1; NZ_JNCM01000036.1.
DR   PDB; 3B3D; X-ray; 2.30 A; A/B/C=1-280.
DR   PDBsum; 3B3D; -.
DR   AlphaFoldDB; O34678; -.
DR   SMR; O34678; -.
DR   STRING; 224308.BSU29050; -.
DR   PaxDb; O34678; -.
DR   PRIDE; O34678; -.
DR   EnsemblBacteria; CAB14865; CAB14865; BSU_29050.
DR   GeneID; 937384; -.
DR   KEGG; bsu:BSU29050; -.
DR   PATRIC; fig|224308.179.peg.3154; -.
DR   eggNOG; COG0656; Bacteria.
DR   InParanoid; O34678; -.
DR   OMA; AEMYANE; -.
DR   PhylomeDB; O34678; -.
DR   BioCyc; BSUB:BSU29050-MON; -.
DR   EvolutionaryTrace; O34678; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   CDD; cd19157; AKR_AKR5G1-3; 1.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR044500; AKR5G.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   PANTHER; PTHR43827; PTHR43827; 1.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..280
FT                   /note="Uncharacterized oxidoreductase YtbE"
FT                   /id="PRO_0000360647"
FT   ACT_SITE        54
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         116
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         194..246
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            83
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250"
FT   STRAND          8..10
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   STRAND          16..20
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           31..43
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   STRAND          47..49
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           52..54
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           57..71
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           75..77
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   STRAND          79..84
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           86..88
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           91..105
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   STRAND          110..117
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   TURN            120..122
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           123..135
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   STRAND          138..146
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           149..155
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   TURN            156..158
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   STRAND          164..169
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           177..186
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   STRAND          189..194
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           197..199
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   TURN            200..203
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           205..213
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           218..228
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           240..247
FT                   /evidence="ECO:0007829|PDB:3B3D"
FT   HELIX           256..263
FT                   /evidence="ECO:0007829|PDB:3B3D"
SQ   SEQUENCE   280 AA;  31768 MW;  D21C6DDF199CB5D0 CRC64;
     MTTHLQAKAT LHNGVEMPWF GLGVFQVEEG SELVNAVKTA IVHGYRSIDT AAIYGNEAGV
     GEGIREGIEE AGISREDLFI TSKVWNADLG YEETLAAFET SLSKLGLDYL DLYLIHWPVE
     GKYKEAWRAL ETLYKEGRIK AIGVSNFQIH HLEDLMTAAE IKPMINQVEF HPRLTQKELI
     RYCQNQGIQM EAWSPLMQGQ LLDHPVLADI AQTYNKSVAQ IILRWDLQHG IITIPKSTKE
     HRIKENASVF DFELTQDDMN RIDALNENLR VGPDPDNFDF