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YTHD2_BOVIN
ID   YTHD2_BOVIN             Reviewed;         580 AA.
AC   Q0VCZ3;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=YTH domain-containing family protein 2 {ECO:0000305};
GN   Name=YTHDF2 {ECO:0000250|UniProtKB:Q91YT7};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal skin;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Specifically recognizes and binds N6-methyladenosine (m6A)-
CC       containing RNAs, and regulates their stability. M6A is a modification
CC       present at internal sites of mRNAs and some non-coding RNAs and plays a
CC       role in mRNA stability and processing. Acts as a regulator of mRNA
CC       stability by promoting degradation of m6A-containing mRNAs via
CC       interaction with the CCR4-NOT and ribonuclease P/MRP complexes,
CC       depending on the context. The YTHDF paralogs (YTHDF1, YTHDF2 and
CC       YTHDF3) share m6A-containing mRNAs targets and act redundantly to
CC       mediate mRNA degradation and cellular differentiation. M6A-containing
CC       mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are
CC       preferentially degraded by endoribonucleolytic cleavage: cooperative
CC       binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment
CC       of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo
CC       deadenylation via direct interaction between YTHDF2 and CNOT1, leading
CC       to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-
CC       containing mRNAs (By similarity). Required maternally to regulate
CC       oocyte maturation: probably acts by binding to m6A-containing mRNAs,
CC       thereby regulating maternal transcript dosage during oocyte maturation,
CC       which is essential for the competence of oocytes to sustain early
CC       zygotic development. Also required during spermatogenesis: regulates
CC       spermagonial adhesion by promoting degradation of m6A-containing
CC       transcripts coding for matrix metallopeptidases (By similarity). Also
CC       involved in hematopoietic stem cells specification by binding to m6A-
CC       containing mRNAs, leading to promote their degradation (By similarity).
CC       Also acts as a regulator of neural development by promoting m6A-
CC       dependent degradation of neural development-related mRNA targets (By
CC       similarity). Inhibits neural specification of induced pluripotent stem
CC       cells by binding to methylated neural-specific mRNAs and promoting
CC       their degradation, thereby restraining neural differentiation.
CC       Regulates circadian regulation of hepatic lipid metabolism: acts by
CC       promoting m6A-dependent degradation of PPARA transcripts. Regulates the
CC       innate immune response to infection by inhibiting the type I interferon
CC       response: acts by binding to m6A-containing IFNB transcripts and
CC       promoting their degradation. May also act as a promoter of cap-
CC       independent mRNA translation following heat shock stress: upon stress,
CC       relocalizes to the nucleus and specifically binds mRNAs with some m6A
CC       methylation mark at their 5'-UTR, protecting demethylation of mRNAs by
CC       FTO, thereby promoting cap-independent mRNA translation. Regulates
CC       mitotic entry by promoting the phase-specific m6A-dependent degradation
CC       of WEE1 transcripts. Promotes formation of phase-separated membraneless
CC       compartments, such as P-bodies or stress granules, by undergoing
CC       liquid-liquid phase separation upon binding to mRNAs containing
CC       multiple m6A-modified residues: polymethylated mRNAs act as a
CC       multivalent scaffold for the binding of YTHDF proteins, juxtaposing
CC       their disordered regions and thereby leading to phase separation. The
CC       resulting mRNA-YTHDF complexes then partition into different endogenous
CC       phase-separated membraneless compartments, such as P-bodies, stress
CC       granules or neuronal RNA granules. May also recognize and bind RNAs
CC       modified by C5-methylcytosine (m5C) and act as a regulator of rRNA
CC       processing (By similarity). {ECO:0000250|UniProtKB:Q91YT7,
CC       ECO:0000250|UniProtKB:Q9Y5A9}.
CC   -!- SUBUNIT: Interacts with CNOT1; interaction is direct and promotes
CC       recruitment of the CCR4-NOT complex. Interacts with YTHDF3. Interacts
CC       with RIDA/HRSP12; interaction leads to recruitment of the ribonuclease
CC       P/MRP complex. {ECO:0000250|UniProtKB:Q9Y5A9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q9Y5A9}. Cytoplasm, P-body
CC       {ECO:0000250|UniProtKB:Q9Y5A9}. Cytoplasm, Stress granule
CC       {ECO:0000250|UniProtKB:Q9Y5A9}. Nucleus {ECO:0000250|UniProtKB:Q9Y5A9}.
CC       Note=Localizes to the cytosol and relocates to the nucleus following
CC       heat shock stress. Can partition into different structures: into P-
CC       bodies in unstressed cells, and into stress granules during stress.
CC       {ECO:0000250|UniProtKB:Q9Y5A9}.
CC   -!- DOMAIN: The disordered regions have the ability to interact with each
CC       other and to 'phase separate' into liquid droplets within the cytosol
CC       following binding to mRNAs containing multiple m6A-modified residues.
CC       This leads to the partition of m6A-containing mRNAs into membraneless
CC       compartments, where mRNAs may be stored, degraded or used to transport
CC       mRNAs to dendritic arbors in neurons. {ECO:0000250|UniProtKB:Q9Y5A9}.
CC   -!- PTM: Ubiquitinated by the SCF(SKP2) complex, leading to its
CC       degradation. {ECO:0000250|UniProtKB:Q9Y5A9}.
CC   -!- SIMILARITY: Belongs to the YTHDF family. YTHDF2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC119920; AAI19921.1; -; mRNA.
DR   RefSeq; NP_001069721.1; NM_001076253.1.
DR   AlphaFoldDB; Q0VCZ3; -.
DR   SMR; Q0VCZ3; -.
DR   STRING; 9913.ENSBTAP00000020940; -.
DR   PaxDb; Q0VCZ3; -.
DR   PRIDE; Q0VCZ3; -.
DR   Ensembl; ENSBTAT00000020940; ENSBTAP00000020940; ENSBTAG00000015771.
DR   GeneID; 541050; -.
DR   KEGG; bta:541050; -.
DR   CTD; 51441; -.
DR   VEuPathDB; HostDB:ENSBTAG00000015771; -.
DR   VGNC; VGNC:37042; YTHDF2.
DR   eggNOG; KOG1901; Eukaryota.
DR   GeneTree; ENSGT00940000156761; -.
DR   HOGENOM; CLU_022715_0_0_1; -.
DR   InParanoid; Q0VCZ3; -.
DR   OMA; RTNGFGD; -.
DR   OrthoDB; 1523251at2759; -.
DR   TreeFam; TF323736; -.
DR   Proteomes; UP000009136; Chromosome 2.
DR   Bgee; ENSBTAG00000015771; Expressed in spermatid and 104 other tissues.
DR   GO; GO:0034451; C:centriolar satellite; IEA:Ensembl.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000932; C:P-body; ISS:UniProtKB.
DR   GO; GO:0062153; F:C5-methylcytidine-containing RNA binding; ISS:UniProtKB.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:1990247; F:N6-methyladenosine-containing RNA binding; ISS:UniProtKB.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0048598; P:embryonic morphogenesis; ISS:UniProtKB.
DR   GO; GO:0098508; P:endothelial to hematopoietic transition; ISS:UniProtKB.
DR   GO; GO:0007276; P:gamete generation; ISS:UniProtKB.
DR   GO; GO:0071425; P:hematopoietic stem cell proliferation; ISS:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0006402; P:mRNA catabolic process; ISS:UniProtKB.
DR   GO; GO:0061157; P:mRNA destabilization; ISS:UniProtKB.
DR   GO; GO:0045746; P:negative regulation of Notch signaling pathway; ISS:UniProtKB.
DR   GO; GO:2000737; P:negative regulation of stem cell differentiation; ISS:UniProtKB.
DR   GO; GO:0060339; P:negative regulation of type I interferon-mediated signaling pathway; ISS:UniProtKB.
DR   GO; GO:0001556; P:oocyte maturation; ISS:UniProtKB.
DR   GO; GO:0070925; P:organelle assembly; ISS:UniProtKB.
DR   GO; GO:1903679; P:positive regulation of cap-independent translational initiation; ISS:UniProtKB.
DR   GO; GO:0030155; P:regulation of cell adhesion; ISS:UniProtKB.
DR   GO; GO:1902036; P:regulation of hematopoietic stem cell differentiation; ISS:UniProtKB.
DR   GO; GO:1903538; P:regulation of meiotic cell cycle process involved in oocyte maturation; ISS:UniProtKB.
DR   GO; GO:0043488; P:regulation of mRNA stability; ISS:UniProtKB.
DR   GO; GO:0050767; P:regulation of neurogenesis; ISS:UniProtKB.
DR   GO; GO:2000232; P:regulation of rRNA processing; ISS:UniProtKB.
DR   GO; GO:0007284; P:spermatogonial cell division; ISS:UniProtKB.
DR   GO; GO:0034063; P:stress granule assembly; ISS:UniProtKB.
DR   InterPro; IPR007275; YTH_domain.
DR   InterPro; IPR045168; YTH_prot.
DR   PANTHER; PTHR12357; PTHR12357; 1.
DR   Pfam; PF04146; YTH; 1.
DR   PROSITE; PS50882; YTH; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Cell cycle; Cell division; Cytoplasm; Differentiation;
KW   Immunity; Innate immunity; Mitosis; Nucleus; Oogenesis; Phosphoprotein;
KW   Reference proteome; RNA-binding; Spermatogenesis; Ubl conjugation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   CHAIN           2..580
FT                   /note="YTH domain-containing family protein 2"
FT                   /id="PRO_0000284978"
FT   DOMAIN          411..545
FT                   /note="YTH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00225"
FT   REGION          1..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2..385
FT                   /note="Localization to mRNA processing bodies (P-bodies)"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   REGION          247..388
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          386..580
FT                   /note="Interaction with m6A-containing mRNAs"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   COMPBIAS        1..25
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        291..326
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        369..383
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         417..419
FT                   /ligand="RNA"
FT                   /ligand_id="ChEBI:CHEBI:33697"
FT                   /ligand_part="N(6)-methyladenosine 5'-phosphate residue"
FT                   /ligand_part_id="ChEBI:CHEBI:74449"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   BINDING         423
FT                   /ligand="RNA"
FT                   /ligand_id="ChEBI:CHEBI:33697"
FT                   /ligand_part="N(6)-methyladenosine 5'-phosphate residue"
FT                   /ligand_part_id="ChEBI:CHEBI:74449"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   BINDING         433..434
FT                   /ligand="RNA"
FT                   /ligand_id="ChEBI:CHEBI:33697"
FT                   /ligand_part="N(6)-methyladenosine 5'-phosphate residue"
FT                   /ligand_part_id="ChEBI:CHEBI:74449"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   BINDING         463
FT                   /ligand="RNA"
FT                   /ligand_id="ChEBI:CHEBI:33697"
FT                   /ligand_part="N(6)-methyladenosine 5'-phosphate residue"
FT                   /ligand_part_id="ChEBI:CHEBI:74449"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   BINDING         487
FT                   /ligand="RNA"
FT                   /ligand_id="ChEBI:CHEBI:33697"
FT                   /ligand_part="N(6)-methyladenosine 5'-phosphate residue"
FT                   /ligand_part_id="ChEBI:CHEBI:74449"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   BINDING         492
FT                   /ligand="RNA"
FT                   /ligand_id="ChEBI:CHEBI:33697"
FT                   /ligand_part="N(6)-methyladenosine 5'-phosphate residue"
FT                   /ligand_part_id="ChEBI:CHEBI:74449"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   MOD_RES         2
FT                   /note="N-acetylserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   MOD_RES         2
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   MOD_RES         4
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   MOD_RES         5
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   MOD_RES         22
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q7Z739"
FT   MOD_RES         39
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   MOD_RES         196
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   MOD_RES         360
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
FT   MOD_RES         395
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y5A9"
SQ   SEQUENCE   580 AA;  62449 MW;  AEF0F9550CFC512F CRC64;
     MSASSLLEQR PKGQGNKVQN GSVHQKDGLN DDDFEPYLSP QARPNNAYTA MSDSYLPSYY
     SPSIGFSYSL GEAAWSTGGD TAMPYLTSYG QLSNGEPHFL PDAMFGQPGA LGSTPFLGQH
     GFNFFPSGID FSAWGNNSSQ GQSTQSSGYS SNYAYAPSSL GGAMIDGQSA FASETLNKAP
     GMNTIDQGMA ALKLGSTEVA SNVPKVVGSA VGSGSITSNI VASNSLPPAT IAPPKPASWA
     DIASKPAKQQ PKLKTKNGIA GSSLPPPPIK HNMDIGTWDN KGPVAKAPSQ ALVQNIGQQP
     TQGSPQPVGQ QANNSPPVAQ ASVGQQTQPL PPPPPQPAQL SVQQQAAQPT RWVAPRNRGS
     GFGHNGVDGN GVGQTQAGSG STPSEPHPVL EKLRSINNYN PKDFDWNLKH GRVFIIKSYS
     EDDIHRSIKY NIWCSTEHGN KRLDAAYRSM NGKGPVYLLF SVNGSGHFCG VAEMKSAVDY
     NTCAGVWSQD KWKGRFDVRW IFVKDVPNSQ LRHIRLENNE NKPVTNSRDT QEVPLEKAKQ
     VLKIIASYKH TTSIFDDFSH YEKRQEEEES VKKERQGRGK
 
 
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