ID   YTPB_BACSU              Reviewed;         367 AA.
AC   O34707;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Tetraprenyl-beta-curcumene synthase;
DE            EC=4.2.3.130 {ECO:0000269|PubMed:21627333};
GN   Name=ytpB; OrderedLocusNames=BSU30500;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT   200 kb rrnB-dnaB region.";
RL   Microbiology 143:3431-3441(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=21627333; DOI=10.1021/ja203779h;
RA   Sato T., Yoshida S., Hoshino H., Tanno M., Nakajima M., Hoshino T.;
RT   "Sesquarterpenes (C35 terpenes) biosynthesized via the cyclization of a
RT   linear C35 isoprenoid by a tetraprenyl-beta-curcumene synthase and a
RT   tetraprenyl-beta-curcumene cyclase: identification of a new terpene
RT   cyclase.";
RL   J. Am. Chem. Soc. 133:9734-9737(2011).
CC   -!- FUNCTION: Catalyzes the transformation of a linear C35 prenyl
CC       diphosphate chain to form tetraprenyl-beta-curcumene.
CC       {ECO:0000269|PubMed:21627333}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-heptaprenyl diphosphate = (R)-tetraprenyl-beta-
CC         curcumene + diphosphate; Xref=Rhea:RHEA:33123, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58206, ChEBI:CHEBI:64801; EC=4.2.3.130;
CC         Evidence={ECO:0000269|PubMed:21627333};
CC   -!- SIMILARITY: Belongs to the large terpene synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AF008220; AAC00246.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15028.1; -; Genomic_DNA.
DR   PIR; E69998; E69998.
DR   RefSeq; NP_390928.1; NC_000964.3.
DR   RefSeq; WP_004399076.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; O34707; -.
DR   SMR; O34707; -.
DR   STRING; 224308.BSU30500; -.
DR   PaxDb; O34707; -.
DR   EnsemblBacteria; CAB15028; CAB15028; BSU_30500.
DR   GeneID; 936222; -.
DR   KEGG; bsu:BSU30500; -.
DR   PATRIC; fig|224308.179.peg.3308; -.
DR   eggNOG; ENOG502Z812; Bacteria.
DR   OMA; WICGLHI; -.
DR   BioCyc; BSUB:BSU30500-MON; -.
DR   BRENDA; 4.2.3.130; 656.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR019712; YtpB-like.
DR   Pfam; PF10776; DUF2600; 1.
PE   1: Evidence at protein level;
KW   Lyase; Reference proteome.
FT   CHAIN           1..367
FT                   /note="Tetraprenyl-beta-curcumene synthase"
FT                   /id="PRO_0000049901"
SQ   SEQUENCE   367 AA;  42843 MW;  7E9CC567E5DB6E4B CRC64;
     MTVPEHPFGL MAKVYRDIFP LVHQELDIWK QKSESIHNSE LKAQATASIR DKTFHCEGGG
     ILALLSGSQK QKCVEFIIAY QTISDYLDNL CDRSTSLDPQ DFRMLHASMQ DALTVGAELQ
     NYYQFREEQD DSGYLHELVK TCQRVLGSIE HYDMIKPYLL ELCGYYCDLQ VHKHVIEHER
     VPRLEKWFTQ YESELPEMEW YEFSACAGST LGIFCLVAYS FQPDFTESTA KKIRDSYFPY
     IQGLHILLDY LIDQEEDLLE GDLNFCSYYQ SHEEMMDRLE HFIHKADEHL QGIPHENFHR
     LINRGLLGVY LSDDKVAGQK EMGRLAKKLI KASGKTSLFF YINGRAYRKF QKMSWMKNSK
     KKAQIIC