ID   YTQA_BACSU              Reviewed;         322 AA.
AC   O35008; Q795P9;
DT   13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Uncharacterized protein YtqA {ECO:0000305};
GN   Name=ytqA; OrderedLocusNames=BSU30480;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT   200 kb rrnB-dnaB region.";
RL   Microbiology 143:3431-3441(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000250|UniProtKB:Q9X0Z6};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000250|UniProtKB:Q9X0Z6};
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. {ECO:0000305}.
CC   -!- CAUTION: The spacing between the conserved Cys residues used for the
CC       4Fe-4S cluster is unusual (C-X11-CXXC instead of C-X3-CXXC); it is
CC       therefore unclear whether this protein will bind the cofactor.
CC       {ECO:0000305}.
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DR   EMBL; AF008220; AAC00380.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15026.1; -; Genomic_DNA.
DR   PIR; D69999; D69999.
DR   RefSeq; NP_390926.1; NC_000964.3.
DR   RefSeq; WP_009968016.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; O35008; -.
DR   SMR; O35008; -.
DR   STRING; 224308.BSU30480; -.
DR   jPOST; O35008; -.
DR   PaxDb; O35008; -.
DR   PRIDE; O35008; -.
DR   EnsemblBacteria; CAB15026; CAB15026; BSU_30480.
DR   GeneID; 937240; -.
DR   KEGG; bsu:BSU30480; -.
DR   PATRIC; fig|224308.179.peg.3306; -.
DR   eggNOG; COG1242; Bacteria.
DR   InParanoid; O35008; -.
DR   OMA; NAGFTCP; -.
DR   PhylomeDB; O35008; -.
DR   BioCyc; BSUB:BSU30480-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.80.30.20; -; 1.
DR   InterPro; IPR039661; ELP3.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR032432; Radical_SAM_C.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR005911; YhcC-like.
DR   PANTHER; PTHR11135; PTHR11135; 1.
DR   PANTHER; PTHR11135:SF1; PTHR11135:SF1; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF16199; Radical_SAM_C; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01091; uncharacterized_CHP01210-like; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR01212; TIGR01212; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..322
FT                   /note="Uncharacterized protein YtqA"
FT                   /id="PRO_0000386530"
FT   DOMAIN          26..267
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         42
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000250|UniProtKB:Q9X0Z6"
FT   BINDING         54
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000250|UniProtKB:Q9X0Z6"
FT   BINDING         57
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000250|UniProtKB:Q9X0Z6"
SQ   SEQUENCE   322 AA;  36764 MW;  99C54EE2020039E4 CRC64;
     MMQNNPFPYS NTEKRYHTLN YHLREHFGHK VFKVALDGGF DCPNRDGTVA HGGCTFCSAA
     GSGDFAGNRT DDLITQFHDI KNRMHEKWKD GKYIAYFQAF TNTHAPVEVL REKFESVLAL
     DDVVGISIAT RPDCLPDDVV DYLAELNERT YLWVELGLQT VHERTALLIN RAHDFNCYVE
     GVNKLRKHGI RVCSHIINGL PLEDRDMMME TAKAVADLDV QGIKIHLLHL LKGTPMVKQY
     EKGKLEFLSQ DDYVQLVCDQ LEIIPPEMIV HRITGDGPIE LMIGPMWSVN KWEVLGAINK
     ELENRGSYQG KFFQRLEEES AL