YUC4_ORYSJ
ID YUC4_ORYSJ Reviewed; 439 AA.
AC Q9LG41;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Indole-3-pyruvate monooxygenase YUCCA4 {ECO:0000305};
DE Short=OsYUCCA4 {ECO:0000303|PubMed:29915329};
DE EC=1.14.13.168 {ECO:0000305|PubMed:29915329};
DE AltName: Full=Flavin-containing monooxygenase YUCCA4 {ECO:0000305};
GN Name=YUCCA4 {ECO:0000303|PubMed:29915329};
GN OrderedLocusNames=Os01g0224700 {ECO:0000312|EMBL:BAF04369.1},
GN LOC_Os01g12490;
GN ORFNames=P0417G05.39 {ECO:0000312|EMBL:BAB07916.2},
GN P0492F05.5 {ECO:0000312|EMBL:BAB32703.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=29915329; DOI=10.1038/s41477-018-0175-0;
RA Song S., Chen Y., Liu L., See Y.H.B., Mao C., Gan Y., Yu H.;
RT "OsFTIP7 determines auxin-mediated anther dehiscence in rice.";
RL Nat. Plants 4:495-504(2018).
CC -!- FUNCTION: Involved in auxin biosynthesis in anthers.
CC {ECO:0000269|PubMed:29915329}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + indole-3-pyruvate + NADPH + O2 = (indol-3-yl)acetate +
CC CO2 + H2O + NADP(+); Xref=Rhea:RHEA:34331, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17640, ChEBI:CHEBI:30854, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.14.13.168;
CC Evidence={ECO:0000305|PubMed:29915329};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC -!- DEVELOPMENTAL STAGE: Highly expressed in anthers at stage 9 of
CC development, before pollen mitotic divisions.
CC {ECO:0000269|PubMed:29915329}.
CC -!- INDUCTION: Repressed by the transcription factor OSH1 during anther
CC development (PubMed:29915329). Reduction of auxin levels at late stage
CC of anther development, after meiosis of microspore mother cells, is
CC necessary for normal anther dehiscence and seed setting
CC (PubMed:29915329). {ECO:0000269|PubMed:29915329}.
CC -!- MISCELLANEOUS: Plants overexpressing YUCCA4 are male sterile due to
CC defect in anther dehiscence and mature pollen grain release at anthesis
CC (PubMed:29915329). Plants silencing YUCCA4 do not exhibit any visible
CC phenotype (PubMed:29915329). {ECO:0000269|PubMed:29915329}.
CC -!- SIMILARITY: Belongs to the FMO family. {ECO:0000305}.
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DR EMBL; AP002835; BAB07916.2; -; Genomic_DNA.
DR EMBL; AP002902; BAB32703.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF04369.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS71105.1; -; Genomic_DNA.
DR EMBL; AK070386; BAG91919.1; -; mRNA.
DR RefSeq; XP_015632133.1; XM_015776647.1.
DR AlphaFoldDB; Q9LG41; -.
DR SMR; Q9LG41; -.
DR STRING; 4530.OS01T0224700-01; -.
DR PaxDb; Q9LG41; -.
DR PRIDE; Q9LG41; -.
DR EnsemblPlants; Os01t0224700-01; Os01t0224700-01; Os01g0224700.
DR GeneID; 4326734; -.
DR Gramene; Os01t0224700-01; Os01t0224700-01; Os01g0224700.
DR KEGG; osa:4326734; -.
DR eggNOG; KOG1399; Eukaryota.
DR HOGENOM; CLU_006909_2_0_1; -.
DR InParanoid; Q9LG41; -.
DR OMA; RSKWLVV; -.
DR OrthoDB; 405736at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR GO; GO:0103075; F:indole-3-pyruvate monooxygenase activity; IDA:UniProtKB.
DR GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR GO; GO:0004499; F:N,N-dimethylaniline monooxygenase activity; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0009901; P:anther dehiscence; IMP:UniProtKB.
DR GO; GO:0009851; P:auxin biosynthetic process; IMP:UniProtKB.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR020946; Flavin_mOase-like.
DR Pfam; PF00743; FMO-like; 1.
DR SUPFAM; SSF51905; SSF51905; 2.
PE 1: Evidence at protein level;
KW FAD; Flavoprotein; Monooxygenase; NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..439
FT /note="Indole-3-pyruvate monooxygenase YUCCA4"
FT /id="PRO_0000445341"
FT BINDING 49..54
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT BINDING 226..231
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000255"
SQ SEQUENCE 439 AA; 48202 MW; 9ABEEA384F86ED1F CRC64;
MDCFAETEGK RAHDPLYQRR AAAAATPATG VPVDDVDKVV DVPGAVIVGA GPAGVAVGAL
LGLRGVAYVV LERCGCIASL WRHRTYDRLC LHLPKRFCEL PLRPFPASFP EYPTRDQFLG
YLDAYAREFG VEPVFRRAVI SAEYDGESWW VYTREVVAAA AGGEQAVLGC TMTVYRSRWL
VVATGENAEP VVPEMDGAGR FKGQMMHSSE YRNGDGYAGK KVLVVGCGNS GMEVSLDLCN
HNARASMVVR DTVHVLPREI LGFSTFGLSM WLLRWLSVQT VDWLVLLLSF LVFGDTARLG
IPRPSLGPFE LKSVSGKTPV LDVGTLAKIK SGDIKVTPAI QCFQEHGVEF VDGSTEEFDV
VILATGYKSN VPYWLKEKEF FSEKDGFPRK GNAWKGQNGL YAVGFSRRGL SGVSMDANNI
VQDIVQRLHD MGYERSENN
