YUC8_ORYSJ
ID YUC8_ORYSJ Reviewed; 421 AA.
AC Q10RE2; Q8H7Y7;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Indole-3-pyruvate monooxygenase YUCCA8 {ECO:0000305};
DE Short=OsYUCCA8 {ECO:0000303|PubMed:29740464};
DE EC=1.14.13.168 {ECO:0000269|PubMed:28829777};
DE AltName: Full=Flavin-containing monooxygenase YUCCA8 {ECO:0000305};
DE AltName: Full=OsFMOt {ECO:0000305};
DE AltName: Full=Protein CONSTITUTIVELY WILTED 1 {ECO:0000303|PubMed:17619151};
DE Short=OsCOW1 {ECO:0000303|PubMed:17619151};
DE AltName: Full=Protein NARROW LEAF 7 {ECO:0000303|PubMed:18293011};
DE AltName: Full=Protein RICE ETHYLENE-INSENSITIVE 7 {ECO:0000303|PubMed:28829777};
GN Name=YUCCA8 {ECO:0000303|PubMed:29740464};
GN Synonyms=COW1 {ECO:0000303|PubMed:17619151},
GN NAL7 {ECO:0000303|PubMed:18293011}, REIN7 {ECO:0000303|PubMed:28829777},
GN YUCA8 {ECO:0000303|PubMed:28829777};
GN OrderedLocusNames=Os03g0162000 {ECO:0000312|EMBL:BAF10962.1},
GN LOC_Os03g06654 {ECO:0000312|EMBL:ABF94120.1};
GN ORFNames=OJ1607A12.11 {ECO:0000312|EMBL:AAN06861.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP MUTAGENESIS OF GLY-201.
RX PubMed=18293011; DOI=10.1007/s00438-008-0328-3;
RA Fujino K., Matsuda Y., Ozawa K., Nishimura T., Koshiba T., Fraaije M.W.,
RA Sekiguchi H.;
RT "NARROW LEAF 7 controls leaf shape mediated by auxin in rice.";
RL Mol. Genet. Genomics 279:499-507(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17619151; DOI=10.1007/s11103-007-9203-6;
RA Woo Y.M., Park H.J., Su'udi M., Yang J.I., Park J.J., Back K., Park Y.M.,
RA An G.;
RT "Constitutively wilted 1, a member of the rice YUCCA gene family, is
RT required for maintaining water homeostasis and an appropriate root to shoot
RT ratio.";
RL Plant Mol. Biol. 65:125-136(2007).
RN [8]
RP FUNCTION, CATALYTIC ACTIVITY, COFACTOR, TISSUE SPECIFICITY, INDUCTION BY
RP ETHYLENE, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Hwayoung;
RX PubMed=28829777; DOI=10.1371/journal.pgen.1006955;
RA Qin H., Zhang Z., Wang J., Chen X., Wei P., Huang R.;
RT "The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is
RT required for ethylene-inhibited root elongation in rice early seedling
RT development.";
RL PLoS Genet. 13:E1006955-E1006955(2017).
RN [9]
RP FUNCTION.
RX PubMed=29740464; DOI=10.3389/fpls.2018.00523;
RA Zhang T., Li R., Xing J., Yan L., Wang R., Zhao Y.;
RT "The YUCCA-auxin-WOX11 module controls crown root development in rice.";
RL Front. Plant Sci. 9:523-523(2018).
CC -!- FUNCTION: Involved in auxin biosynthesis (PubMed:18293011,
CC PubMed:28829777, PubMed:29740464). Converts the indole-3-pyruvic acid
CC (IPA) produced by the TAA family to indole-3-acetic acid (IAA)
CC (PubMed:28829777). Seems not able to use tryptamine (TAM) as substrate
CC (PubMed:28829777). Probably responsible for auxin biosynthesis in
CC leaves and involved in the regulation of lateral leaf growth
CC (PubMed:18293011). Required for maintaining water homeostasis and an
CC appropriate root to shoot ratio (PubMed:17619151). Required for the
CC inhibition of root growth by ethylene in etiolated seedlings
CC (PubMed:28829777). Functions downstream of the ethylene-response
CC transcription factor EIL1 (PubMed:28829777).
CC {ECO:0000269|PubMed:17619151, ECO:0000269|PubMed:18293011,
CC ECO:0000269|PubMed:28829777, ECO:0000269|PubMed:29740464}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + indole-3-pyruvate + NADPH + O2 = (indol-3-yl)acetate +
CC CO2 + H2O + NADP(+); Xref=Rhea:RHEA:34331, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17640, ChEBI:CHEBI:30854, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.14.13.168;
CC Evidence={ECO:0000269|PubMed:28829777};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000269|PubMed:28829777};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000269|PubMed:17619151}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, young panicles and young
CC kernels (PubMed:17619151). Highly expressed in embryos. Expressed in
CC roots, shoots and panicles (PubMed:18293011). Expressed in root tips,
CC leaves, stems, young stem nodes and developing grains
CC (PubMed:28829777). {ECO:0000269|PubMed:17619151,
CC ECO:0000269|PubMed:18293011, ECO:0000269|PubMed:28829777}.
CC -!- INDUCTION: Induced by ethylene. {ECO:0000269|PubMed:28829777}.
CC -!- DISRUPTION PHENOTYPE: Narrow and rolled leaf phenotype
CC (PubMed:17619151, PubMed:28829777). Reduced root growth
CC (PubMed:17619151). Tolerance to salt stress (PubMed:28829777). Reduced
CC response to inhibition of root elongation by ethylene
CC (PubMed:28829777). {ECO:0000269|PubMed:17619151,
CC ECO:0000269|PubMed:28829777}.
CC -!- MISCELLANEOUS: Calli overexpressing YUCCA8 exhibit high levels of
CC auxin, low regeneration frequency, overgrowing roots and abnormal root
CC morphology. {ECO:0000269|PubMed:18293011, ECO:0000269|PubMed:28829777,
CC ECO:0000269|PubMed:29740464}.
CC -!- SIMILARITY: Belongs to the FMO family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN06861.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB354302; BAG14341.1; -; Genomic_DNA.
DR EMBL; AC105729; AAN06861.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000009; ABF94120.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF10962.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82425.1; -; Genomic_DNA.
DR EMBL; AK072466; BAG92984.1; -; mRNA.
DR RefSeq; XP_015631027.1; XM_015775541.1.
DR RefSeq; XP_015631028.1; XM_015775542.1.
DR RefSeq; XP_015631029.1; XM_015775543.1.
DR AlphaFoldDB; Q10RE2; -.
DR SMR; Q10RE2; -.
DR STRING; 4530.OS03T0162000-01; -.
DR PaxDb; Q10RE2; -.
DR PRIDE; Q10RE2; -.
DR EnsemblPlants; Os03t0162000-01; Os03t0162000-01; Os03g0162000.
DR GeneID; 4331709; -.
DR Gramene; Os03t0162000-01; Os03t0162000-01; Os03g0162000.
DR eggNOG; KOG1399; Eukaryota.
DR HOGENOM; CLU_006909_2_0_1; -.
DR InParanoid; Q10RE2; -.
DR OMA; CELPHAR; -.
DR OrthoDB; 405736at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR GO; GO:0103075; F:indole-3-pyruvate monooxygenase activity; IDA:UniProtKB.
DR GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR GO; GO:0004499; F:N,N-dimethylaniline monooxygenase activity; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0009851; P:auxin biosynthetic process; IMP:UniProtKB.
DR GO; GO:2000024; P:regulation of leaf development; IMP:UniProtKB.
DR GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR020946; Flavin_mOase-like.
DR Pfam; PF00743; FMO-like; 1.
DR SUPFAM; SSF51905; SSF51905; 2.
PE 1: Evidence at protein level;
KW Auxin biosynthesis; Endoplasmic reticulum; FAD; Flavoprotein;
KW Monooxygenase; NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..421
FT /note="Indole-3-pyruvate monooxygenase YUCCA8"
FT /id="PRO_0000445280"
FT BINDING 30..35
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT BINDING 201..206
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000255"
FT MUTAGEN 201
FT /note="G->V: In nal7; narrow and culred leaf phenotype."
FT /evidence="ECO:0000269|PubMed:17619151"
SQ SEQUENCE 421 AA; 45720 MW; 02974A5ACF7DAE3D CRC64;
MQGQQKQNAG GGGGDNASPC IVLDGPIIVG AGPSGLAVAA TLRQHGAPFT VVERSGGVAD
LWTNRTYDRL RLHLPKVFCE LPHVAFPPDF PTYPTKHDFL RYLHSYAARF AIAPLLRRTV
TRAWYDHPAS LWRVTTTTTS SSATSVITEY ASPWLVVASG ENAEVVVPKV KGRERFAGEA
LHSSEYRSGE RFRGMRVLVV GCGNSGMEMC LDLCEHGAMP FMSVRSGVHV LPREMFGAST
FGIAMKLLRW LPIKMVDRFL LLVARMVLGD TEKYGLKRPK LGPLEIKNIT GKSPVLDVGA
WSLIKSGNIK IVPEVESFSG NGARFVDGNE MAFDAVIFAT GYRSNVPSWL QEDGELFTEE
GKLRSSGSSS EWRWRGPNGL YCVGFSGRGL LGAGADALRA AADIAGRWQE TQQAAANISS
V
